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Showing: 601 - 620 of 781 results

  1. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ... but they are not recommended for tumorigenic compounds, as circulation is slower, and more institutional...depending on researcher preferences. It sounds complicated but has a pretty low learning curve! Restraint...

  2. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...plants, mammals, and insects. In this post, we will compare two of the most widely used systems, mammalian ... from dedicated adenoviral production labs or companies. Production of both AAV and lentivirus requires...

  3. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...or producer cells for further processing. What component(s) you need to collect will depend on the serotype...Absolute determination of single-stranded and self-complementary adeno-associated viral vector genome titers ...

  4. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...practice while seated or with your poster on your computer screen than it is to pretend you’re standing at...guilty of this, and have many times answered a completely different question than the intended one. Take...

  6. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ...protein fusion. CRISPR genome editing can help accomplish this goal. The power of this system lies in the...PMID: 27052166. Kunzelmann, Stefan, et al. “A Comprehensive Toolbox for Genome Editing in Cultured Drosophila...

  7. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...few new ones that are not used so much but have comparable expression levels to things people do use a lot...example, knock out a gene with simultaneous complementation, with a knockout and expression construct on...

  8. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...correct genetic conditions over 25 years ago when completing her PhD and medical training in Ophthalmology...time. Importantly, it is an immune privileged compartment, meaning that it can tolerate the presence of...

  9. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ... or very similar buffers if not, since buffer components can affect the final readout. When preparing ...using advanced graphing software designed to plot complex trendlines. If you don’t have access to this kind...

  10. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...literature and emerging technologies to remain competitive and relevant. Enter: the journal club.  A journal...gauged interest from relevant parties, and the company at large, by polling and informal communications...

  11. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...end joining (alt-EJ). Inhibitors to essential components of both pathways have been shown to be efficacious... al., Nat Com).     Fig. 3: HDR events compete with NHEJ-mediated edits of small insertions and...

  12. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ..., but it still points to a far larger and more complex problem endemic to biomedical research: pervasive...same reference genomes and libraries. Further complicating the issue is the long and ugly history of reporting...

  13. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...replication that can be packaged into replication-incompetent phage particles using a helper system. These ...antimicrobials combines them with both replication-competent phages and antibiotics. Phage therapy fell out...

  14. Tips for Screening with Yeast Two Hybrid Systems

    Type
    Blog Post
    Published
    Oct. 22, 2015, 2:30 p.m.
    ...transcription. The HIS3 reporter system (e.g. the Gateway-compatible bait Y2H plasmid pEZY202  from Yu-Zhu Zhang’s...particular cell type, or a subset based on a cellular compartment or growth stage). A prey library can either be...

  15. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    Published
    April 1, 2025, 1:15 p.m.
    ... Note: hypoxanthine (shown) is the nucleobase component of the nucleoside inosine. Created with BioRender.com...relied on deaminase enzymes, either to directly accomplish the base edit or to create the substrate for ...

  16. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ...in mice is possible (Guy et al., 2007). They accomplished this using Cre-Lox mouse models in which the...Cre not only halted the neurological damage but completely reversed it, regardless of age or symptom severity...

  18. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ...varied codon preferences among organisms aren’t completely understood, but some possible reasons include...codon optimization algorithms and DNA synthesis companies such as IDT and GenScript host online codon optimization...

  19. Antibodies 101: Reading a Flow Plot

    Type
    Blog Post
    Published
    Feb. 6, 2024, 3:13 p.m.
    ...roughly measures granularity, a proxy for nucleic complexity of the cell.  The sample will have been labeled...labeled using a panel of markers, which is comprised of antibodies conjugated to fluorescent markers and/...
Showing: 601 - 620 of 781 results