We narrowed to 805 results for: Cre

...for NRN PAMs and to a lesser extent NYN. This increases the flexibility of gRNA placement significantly...NYN PAMs; it just isn’t always ideal. But the increased flexibility helps even when it’s not an ideal ...

..., use a broadly reactive secondary antibody to increase the chance of primary/secondary antibody binding...cross-adsorption process filters out off-target antibodies increasing specificity and reducing cross-reactivity. For...

...-pot system will allow investigators to rapidly create multiple synthetic chromatin-modifying proteins...cells (Zhang et al., 2015). Recently the Ward lab created a tool-kit to expand upon these original tools ...

...PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from...mediated editing, as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the...

...resulted in a cell migration defect. They have created a circRNA website so scientists can view public...developments. Using the Tet-on system, his lab has created a tetracycline cas9 inducible knock-out system....

... with diamond polishing paper of sequentially increasing fineness helps to maintain a consistent stream... rate of movement of the animal significantly increases. Before you get your experiment started, you will...

...discussion will get side-tracked, and you might create even more conflicts. 4) Define in advance how you...graduate students since 2009. Dora is also the creator of the “Finish Your Thesis” online course.  To ...

...of both while limiting disadvantages led to the creation of induced pluripotent stem cells or iPSCs. Induced...research. With all these benefits, iPSCs have increasingly become the ideal experimental system for research...

... their restriction sites allowing you to easily create customized overhangs to assemble up to 6 different...number of fragments you’re hoping to assemble increases. Multiple inserts: There are some cloning methods...

...your team you will have a much better chance of creating a high-performing team - which is every manager...! Remember that delegating is not just a way to create more time for yourself, but it will help develop...

...better suited for genome-wide CRISPRi library screening experiments where maintaining library diversity...of antibiotics are often essential genes which creates a challenge when studying them since their complete...

...the sequence inserted by HDR may inadvertently re-create a match for the active guide. With these constraints.... To use this tool, simply follow these steps: Create a new experiment in the program Design gRNAs by...

... you have either made a pipetting error or an incredible discovery. The range of RQ values used to call...to be amplified in the same reaction. Taqman’s increased sensitivity allows for a more confident determination...

...This amplicon can be used in molecular cloning to create a plasmid of interest, for detecting the presence...detected via a fluorescent signal. The signal increases proportionally to the amount of product produced...

...last decade, the use of degron tags has become increasingly popular for the modulation of endogenously and...have a kit!). Choosing a degron tag Are all tags created equal? No! Choosing the ‘right’ tag does depend...

...Pre-made viruses have become increasingly accessible and are useful for saving time and avoiding potentially... collected over the course of a couple days to increase yield. The collection process is a cycle of collecting...

...a lot of attention on social media. I gained a concrete example to use to promote future attempts and ...learning from the careers of others were key to creating my career path. Luckily, in grad school much of...

...gRNA.  While this methodology has proven to be incredibly valuable to scientists, there are some potential...Harrington, Lucas B., et al. "A thermostable Cas9 with increased lifetime in human plasma." Nature Communications8.1...

... itself in terms of what they do with their discretionary money. They also usually have a good event-planning...students, postdocs, and other working scientists, creating a presentation for 8th graders was a little intimidating...

...researcher experiences and that it often provides incredibly valuable insight into one’s work. As a community...information can be cited, tracked, and appropriately credited, broadening the knowledge base of AAV vector performance...