We narrowed to 700 results for: EED

1. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

   Type

   Blog Post

   Published

   May 26, 2020, 1:15 p.m.

   ...successful CRISPR editing experiments, there was a real need in the scientific community for efficient real-time...

2. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

   Type

   Blog Post

   Published

   Jan. 30, 2020, 2:15 p.m.

   ...regardless of outcome, and are able to receive feedback on their study plans at a point in the research...

3. Why and how to start studying chromatin (with Addgene's help)

   Type

   Blog Post

   Published

   June 12, 2018, 11:40 a.m.

   ...methylation status at a double-strand break that needs repairing. They may be looking at chromatin at the...

4. Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering

   Type

   Blog Post

   Published

   May 3, 2018, 1:48 p.m.

   ...characteristic of the Cas13 superfamily. Yan et al. proceeded to study orthologs from Eubacterium siraeum (EsCas13d...

5. Building and Validating Recombinant Antibodies: A Case Study

   Type

   Blog Post

   Published

   April 5, 2022, 1:20 p.m.

   ... This greatly limited the number of clones that needed to be screened through functional assays. Following...

6. Antibodies 101: Monoclonal Antibodies

   Type

   Blog Post

   Published

   June 29, 2021, 1:15 p.m.

   ...you make the best decision for your experimental needs.     References and resources References Bradbury...

7. A Deep Dive into BioBuilder

   Type

   Blog Post

   Published

   Oct. 11, 2017, 1:44 p.m.

   ... workshops, and customized programs to meet the needs of students and teachers. It’s possible to join ...

8. A Practical Approach to Choosing the B(right)est Fluorescent Protein

   Type

   Blog Post

   Published

   June 1, 2017, 2:30 p.m.

   ... best choice, the fluorescent protein properties need to be scrutinized under relevant conditions. The...

9. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ..., reversible activation of AgRP neurons drives feeding behavior in mice. J Clin Invest. 2011 Apr;121(4...

10. OCaMP: A New Calcium Indicator for Neural Imaging

    Type

    Blog Post

    Published

    Aug. 19, 2025, 1:15 p.m.

    ... in the brain. For this to work, each indicator needs to emit a different color of light and be compatible...

11. Fluorescent Proteins 101: Luciferases

    Type

    Blog Post

    Published

    July 22, 2025, 1:15 p.m.

    ..., which emits light. No excitation illumination needed — just the luminescent substrate. This approach...

12. Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

    Type

    Blog Post

    Published

    Nov. 5, 2019, 1:28 p.m.

    ...CRISPR nucleases for many more specific research needs. In this update, we will focus on the most recent...

13. Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection

    Type

    Blog Post

    Published

    June 25, 2019, 12:54 p.m.

    ...cell. Microinjection requires the use of a fine needle to deliver nucleic acids to individual cells. Electroporation...

14. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

    Type

    Blog Post

    Published

    June 6, 2019, 12:41 p.m.

    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

15. Early Career Researcher Toolbox: Social Media for Scientists

    Type

    Blog Post

    Published

    Dec. 11, 2018, 12:46 p.m.

    ...are using social media professionally, and 2) I needed to start using Twitter. Sharing Chemistry Cat...

16. Plasmids 101: Blue-white Screening

    Type

    Blog Post

    Published

    June 4, 2015, 1:03 p.m.

    ...plates: X-gal is light and temperature sensitive and needs to be added to media after autoclaving. If spread...

17. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type

    Blog Post

    Published

    June 2, 2015, 6:06 p.m.

    ...also a factor; the pol lll promoter and terminator need be encoded only once, and each <200 bp tRNA-gRNA...

18. CRISPR 101: Non-Homologous End Joining

    Type

    Blog Post

    Published

    April 16, 2015, 3:45 p.m.

    ... common to biological sources of DSBs, and which need to be processed. Processing occurs prior to ligation...

19. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type

    Blog Post

    Published

    Feb. 18, 2014, 4:22 p.m.

    ...formally often enough) and give honest, critical feedback for improvement. Go to a science or career seminar...

20. RNA Extraction Without A Kit

    Type

    Blog Post

    Published

    March 24, 2020, 1:15 p.m.

    ...foremost, be mindful of your extractions- if you need really clean RNA, it’s important to make sure that...