We narrowed to 694 results for: EED
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TypeBlog PostPublishedDec. 11, 2018, 12:46 p.m....are using social media professionally, and 2) I needed to start using Twitter. Sharing Chemistry Cat...
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Why and how to start studying chromatin (with Addgene's help)
TypeBlog PostPublishedJune 12, 2018, 11:40 a.m....methylation status at a double-strand break that needs repairing. They may be looking at chromatin at the... -
Cas13d: Small RNA-targeting CRISPR enzymes for transcriptome engineering
TypeBlog PostPublishedMay 3, 2018, 1:48 p.m....characteristic of the Cas13 superfamily. Yan et al. proceeded to study orthologs from Eubacterium siraeum (EsCas13d... -
A Deep Dive into BioBuilder
TypeBlog PostPublishedOct. 11, 2017, 1:44 p.m.... workshops, and customized programs to meet the needs of students and teachers. It’s possible to join ... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog PostPublishedJune 1, 2017, 2:30 p.m.... best choice, the fluorescent protein properties need to be scrutinized under relevant conditions. The... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m...., reversible activation of AgRP neurons drives feeding behavior in mice. J Clin Invest. 2011 Apr;121(4... -
Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2
TypeBlog PostPublishedMay 26, 2020, 1:15 p.m....successful CRISPR editing experiments, there was a real need in the scientific community for efficient real-time... -
The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It
TypeBlog PostPublishedJan. 30, 2020, 2:15 p.m....regardless of outcome, and are able to receive feedback on their study plans at a point in the research... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m.... This greatly limited the number of clones that needed to be screened through functional assays. Following... -
Antibodies 101: Monoclonal Antibodies
TypeBlog PostPublishedJune 29, 2021, 1:15 p.m....you make the best decision for your experimental needs. References and resources References Bradbury... -
Fluorescent Proteins 101: Luciferases
TypeBlog PostPublishedJuly 22, 2025, 1:15 p.m...., which emits light. No excitation illumination needed — just the luminescent substrate. This approach... -
Plasmids 101: Blue-white Screening
TypeBlog PostPublishedJune 4, 2015, 1:03 p.m....plates: X-gal is light and temperature sensitive and needs to be added to media after autoclaving. If spread... -
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs
TypeBlog PostPublishedJune 2, 2015, 6:06 p.m....also a factor; the pol lll promoter and terminator need be encoded only once, and each <200 bp tRNA-gRNA... -
CRISPR 101: Non-Homologous End Joining
TypeBlog PostPublishedApril 16, 2015, 3:45 p.m.... common to biological sources of DSBs, and which need to be processed. Processing occurs prior to ligation... -
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists
TypeBlog PostPublishedFeb. 18, 2014, 4:22 p.m....formally often enough) and give honest, critical feedback for improvement. Go to a science or career seminar... -
Seven Tips for Using LinkedIn as a Scientist
TypeBlog PostPublishedOct. 1, 2019, 1:21 p.m....on LinkedIn?” For anyone who is planning or might need to plan for a career outside academia (that would... -
Plasmid Tools for Microbiome Studies
TypeBlog PostPublishedJune 27, 2019, 12:25 p.m....diverse species. These results further highlight the need for in situ genetic tools for microbiome studies... -
RaPID Detection of RNA-protein Interactions
TypeBlog PostPublishedAug. 16, 2018, 12:40 p.m....detect RNA-protein interactions. To address this need, the Khavari lab at Stanford created the RNA-protein... -
RANbodies: Reporter Nanobody Fusions
TypeBlog PostPublishedApril 10, 2018, 12:56 p.m.... DNA ladders with these plasmids from Penn State Need help picking the right fluorescence microscopy technique... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m.... directed to the proper target sequence. They succeeded in labeling two different pairs of chromosomes...