We narrowed to 685 results for: Eng

1. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ...Galvan et al., 2019). The hM4Di receptor is an engineered Gi protein-coupled receptor, one of the Designer...

2. RNA Extraction Without A Kit

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   Blog Post

   Published

   March 24, 2020, 1:15 p.m.

   ...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

3. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   Published

   March 3, 2020, 2:15 p.m.

   ...editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural...

4. Reproducibility for Everyone: Lessons from an Open Science Collaboration

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   Blog Post

   Published

   Feb. 28, 2019, 1:34 p.m.

   ...working with communities, not for them - Another strength of this collaboration is that it is driven by ...

5. Human Germline Editing Using CRISPR

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   Blog Post

   Published

   Aug. 10, 2017, 2:19 p.m.

   ...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...

6. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   Published

   June 15, 2017, 2:30 p.m.

   ...new plasmids and/or cells or organisms that are engineered can be shared to simplify the comparison by others...

7. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

   Type

   Blog Post

   Published

   April 13, 2017, 2:30 p.m.

   ...~6.5 - 9 *Optimal sequential excitations wavelengths as determined by Rosado et al: see Table 2 for...

8. Developing Lab Management Software for Biology

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   Blog Post

   Published

   Aug. 25, 2016, 2:30 p.m.

   ...in on the fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software...

9. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

   Type

   Blog Post

   Published

   June 26, 2018, 1:33 p.m.

   ...features that allow such “chemical rescue” in vivo. Engineering the human disease alleles into cells for testing...

10. Adenoviral Vector Production and Troubleshooting

    Type

    Blog Post

    Published

    June 14, 2018, 11:44 a.m.

    ...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...

11. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type

    Blog Post

    Published

    May 24, 2018, 1:20 p.m.

    ...experiments are either not accessible or too challenging for amateur scientists. Planarians, wormy creatures...

12. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type

    Blog Post

    Published

    Oct. 22, 2019, 12:20 p.m.

    ...outside of the reader’s field of view because of its length, and will be more difficult to read. The Thompson...

13. Viral Vectors 101: Chemogenetics

    Type

    Blog Post

    Published

    July 11, 2023, 1:15 p.m.

    ...Chemogenetics relies on genetically introducing engineered receptors that are only activated by otherwise...

14. Antibodies 101: Choosing the Right Isotype

    Type

    Blog Post

    Published

    April 6, 2023, 3 p.m.

    ...secondary antibody binding and therefore the signal strength (Fig. 1). Monoclonal primaries If you do decide...

15. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...

16. A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells

    Type

    Blog Post

    Published

    May 7, 2020, 1:15 p.m.

    ...T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in primary...

17. Antibodies 101: Stripping and Reprobing Western Blots

    Type

    Blog Post

    Published

    Aug. 1, 2024, 1:15 p.m.

    ...time you strip a blot, you should not compare the strength of bands from different rounds of detection. Finally...

18. 6 Tips for Grant Writing

    Type

    Blog Post

    Published

    Oct. 18, 2016, 2:30 p.m.

    ...approach that problem in a novel way. Finally, engineering new applications to solve problems will provide...

19. Adapting Toehold Switches to Detect Zika Virus

    Type

    Blog Post

    Published

    Aug. 30, 2016, 2:30 p.m.

    ...2014, as postdoctoral fellows with Jim Collins and Peng Yin at the Wyss Institute (Harvard University), ...

20. 10 Basic tips for mammalian cell culture

    Type

    Blog Post

    Published

    July 12, 2018, 1:09 p.m.

    ...Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian Cells with ...