We narrowed to 687 results for: Eng

...until homogenization and cell lysis. This can be challenging depending on your laboratory situation or tissue...

...editors at Addgene. Levy et al., Nature Biomedical Engineering, 2020. PubMed PMID: 31937940. Detection of neural...

...structural knowledge of the proteins. To overcome this challenge, the Uppalapati and Woolley lab developed a phage...

...working with communities, not for them - Another strength of this collaboration is that it is driven by ...

...bind an RNA motif of interest in living cells. Challenges with existing RNA-protein interaction methods...

...and feedback giving authors the opportunity to strengthen the manuscript prior to submission. Preprints...

...heterozygous mutations, it will clearly be much more challenging for homozygous mutations. Moreover, introducing...

...new plasmids and/or cells or organisms that are engineered can be shared to simplify the comparison by others...

...~6.5 - 9 *Optimal sequential excitations wavelengths as determined by Rosado et al: see Table 2 for...

...in on the fun too. Kris Shamloo is a Software Engineer at Addgene and is interested in writing software...

...Chemogenetics relies on genetically introducing engineered receptors that are only activated by otherwise...

...secondary antibody binding and therefore the signal strength (Fig. 1). Monoclonal primaries If you do decide...

...DNA Extracts. Pooled libraries are a bit more challenging to QC by NGS as they are often quite different...

...time you strip a blot, you should not compare the strength of bands from different rounds of detection. Finally...

...T-to-G•C base changes (Figure 1), but it can be challenging to make these base changes, especially in primary...

...features that allow such “chemical rescue” in vivo. Engineering the human disease alleles into cells for testing...

...not integrate into the host’s genome. The main challenge with using AdV is that it triggers a strong immune...

...experiments are either not accessible or too challenging for amateur scientists. Planarians, wormy creatures...

...outside of the reader’s field of view because of its length, and will be more difficult to read. The Thompson...

...approach that problem in a novel way. Finally, engineering new applications to solve problems will provide...