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We narrowed to 878 results for: IND

Showing: 601 - 620 of 878 results

  1. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...you have to know what resources exist and where to find them! Keeping good records and an organized lab ... are typically more expensive, so you’ll have to find a balance between time savings and budget savings...factors to help you choose the right cloning method Find blog posts about scientific sharing here Download... out our special collection pages to more easily find plasmids Search for a plasmid in Addgene’s repository...

  2. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...some of its dsRNA intermediates into a healthy individual to provoke an immune response and then, hopefully...degrade mRNA transcripts. RNAi activates the RNA-induced silencing complexes (RISCs), which detect dsRNA...dysfunction, it is unclear what the precise mechanism behind the production of these dsRNAs is. A major source...infection. Similarly, if endogenously synthesized dsRNA finds its way outside of the mitochondria, the same inflammatory...

  3. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...neurons are likely the first thing that comes to mind. However, ion-based communication is not just for...reporting and genetically encoded fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric states...channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization in continuous...BLINK2 potassium channel - An optimized blue-light induced synthetic potassium channel. Upon activation, it...

  4. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...sequence information. Collect plasmid information in a binder or digital notebook and make sure you note the ...deposit your plasmids with us, it will be easy to find all of the information needed for the process. Always...primer, you need some space between your primer binding site and the first feature you want to verify because...this region, as outlined above. Avoid primers that bind within or must read through repetitive regions (...

  5. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ... are also beginning to address these issues independently; for example, Harvard’s Therapeutics Graduate... has a compulsory internship to be taken in an industry, regulatory, or clinical environment. It remains...again gather in Boston - this time with the aim of finding solutions to some of the problems that were identified...there for STEM graduates and postdocs in academia, industry and other areas of employment? How many graduates...

  6. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...with a body of literature related to the topic. Finding reliable references and sorting out what they mean...Searching for literature My go-to search engines for finding scientific literature are Google Scholar and PubMed...When embarking on a new literature search, try to find a recent review article to give you an overview ...data. They should explain some of the rationale behind the line of experiments, what data they collected...

  7. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ... developed to treat a multitude of therapeutic indices, including hypertension, cancer, diabetes, cardiovascular...more than 300 different coactivator proteins individually contribute to cell regulation in a coherent ...and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear Receptor Signaling. Dr. McKenna...and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear Receptor Signaling.   Further ...

  8. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...methionine (i.e. the plasmid complements the deletion). Find the Plasmids Here Plasmid features enable multiple...met deficiencies (manuscript in preparation), indicating that Met protein functions have been largely ... in communicating science to the public. You can find out more about Clare at the Biology Dept. site.  ...Explore Careers at Addgene Resources on Addgene.org Find Yeast Expression Vectors Check Out Our Molecular...

  9. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ... new blog post, and live tweet from conferences. Find more tips on using social media as a scientist here...Network – Mingle with pros Where do you meet like-minded science communicators? Attend the annual flagship...started writing three years ago on a field trip in Indonesia. Since then, I have tried many things: photography...Communication Additional Resources on Addgene.org Find Job Openings at Addgene ...

  10. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...poster In almost all scientific posters you will find a title, author names and affiliations, introduction...University. The idea is that the main research finding is written across the center of the poster in a...be cognizant of audience members that are color-blind; avoiding red and green is a good idea for this ... this blog post on navigating the poster session Find tips to make the most of your conference attendance...

  11. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...access to some cells and tissues, such as human induced pluripotent stem cells (hiPSCs). Nanoblades, a ...different types of CRISPR cargo including RNPs for indel formation via NHEJ, precise modification with Homology...was not detected and Nanoblade injection did not induce morbidity. Together these results demonstrate that...Addgene.org Check out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral ...

  12. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...protein variant lacking its endonuclease ability, to bind to genes without editing the genome (Qi et al., ...complementary to the sequence of interest. Upon binding, CRISPRa systems recruit transcription factors ...VP64 fusion protein engineered with aptamers that bind to MS2 proteins. These MS2 proteins then recruit...Resources on Addene.org Read Addgene's CRISPR Guide Find CRISPR plasmids for your research ...

  13. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...during undergrad. Lauren Flynn is the Addgenie behind our "Meet an Addgenie" series. Before heading off...is a more emphasis today about non-academia and industry jobs. Since there are a variety of different career...can make big changes in your career path and still find comfort and happiness there. Read all of the Meet...process, my advisor encouraged me to keep my values in mind as I applied. Thinking about this made me curious...

  14. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ... is a small piece of DNA that can replicate independently from its host’s chromosome. Although we usually...of conjugation, and these are encoded by certain kinds of plasmids — more on that in a minute! Fun fact...antibiotic resistance-mediated selection is indispensable for cloning work, antibiotics and plasmid-encoded...biology, especially when combined with natural inducible expression systems that allow tuning of metabolic...

  15. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...given site. While it is possible to "get lucky" and find a great gRNA with a proximal PAM right where you...solution to the PAM problem seems simple, in theory: find a Cas enzyme or evolve one that uses a PAM with ...flexibility could in theory promote off-target binding. The activity of SpRY was validated in double stranded... the genome! Want to get your hands on SpRY? Find a mammalian plasmid here and edit on! References...

  16. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...these tools with the Zika virus research community. Find the Zika Sensor Constructs at Addgene As we report...forward, our short-term efforts are focused on finding the resources necessary to advance the technology...Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids from the publication at Addgene 2. Green...Check out All the Plasmids from the Collin's Lab Find Synthetic Biology Plasmids for Your Next Experiment...

  17. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...technical challenges they can present. Many researchers find themselves plagued by troubleshooting a virus-based... during transfection, and does not necessarily indicate the production of virus. Alternatively, if the...gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes." Biophysical...Addgene.org Check out Our Viral Vector Guide Pages Find Virus Protocols Order Virus ...

  18. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...become increasingly restricted to academic and industrial labs, but recently there has been a counter movement...bioinformatics pipeline. It’s possible that we’ll find something interesting and contribute it back to ...when starting a new project at a makerspace is finding the financial resources to pursue it. Since makerspaces...area, please stop by. And if not, I suspect you can find a makerspace somewhere near you. The place to start...

  19. How-to: 5 Steps to a Great Panel Discussion

    Type
    Blog Post
    Published
    Oct. 22, 2014, 1:57 p.m.
    ... way to bring out these interesting stories and remind the panelists of the stories or topics you know...correct date, location and parking information and remind them about the event as it approaches. Room set-up...if slides are allowed. Don't put your panelists behind a table. It adds a formality and distance that ...few questions. Who is out there? What do they do? Find out their areas of science or expertise by listing...

  20. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ... Off Resources on Addgene.org Find Mammalian Expression Vectors Find Cre-Lox Plasmids Learn More about...with a gRNA). Be sure to keep these techniques in mind when choosing a knockout strategy! Knockout plasmids...knocked out. Use positive and negative selection to find correctly recombined cells. Recombination is a rare...
Showing: 601 - 620 of 878 results