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We narrowed to 889 results for: IND

Showing: 601 - 620 of 889 results

  1. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...manuscript to make resources easier to identify, find back, and share. Depending on the type of manuscript...including F1000Research, also ask for the raw data behind the figures and results. This is a relatively new...researchers to access the data and make up their own minds on whether the data analysis has been of good quality...accepted for publication and is, in most cases, blind (meaning you don’t know who the reviewers are). ...

  2. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type
    Blog Post
    Published
    Oct. 29, 2019, 2:52 p.m.
    ...neurons are likely the first thing that comes to mind. However, ion-based communication is not just for...reporting and genetically encoded fluorescent voltage indicators (GEVI) are ideal tools to track bioelectric states...channelrhodopsin used as a sensitive fluorescent voltage indicator with near-complete desensitization in continuous...BLINK2 potassium channel - An optimized blue-light induced synthetic potassium channel. Upon activation, it...

  3. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ... on the Addgene Blog Find tips for online social networking for scientists Find tools for using social...profile is absolutely necessary. If people can’t find you on LinkedIn, you will lose opportunities and...for politics, family updates, or click bait of any kind. For more on inappropriate content see this article...

  4. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...Addgene.org Find protocols for plasmid cloning Read our molecular biology reference Find how-to videos..., Gibson Assembly, Golden Gate etc.), and try to find the simplest way to do it (i.e. avoid assembling...cloning strategy: Restriction enzyme digestion Be mindful of the restriction enzymes you chose. Make sure...

  5. The Breast Cancer Microenvironment: A Tumor’s Backstage Team

    Type
    Blog Post
    Published
    Jan. 31, 2019, 1:32 p.m.
    ..., the lack of caveolin 1 in breast cancer also induces changes of stromal cells in the microenvironment...transforming growth factor-β signaling, possibly inducing the secretion of growth promoting proteins, such...molecules that can be transferred to other cells, inducing epigenetic changes in targeted cells. In this ...our cancer research plasmids and resources page  Find plasmids for your research Learn about Addgene's...

  6. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...manuscripts shared by the authors on a public server independent from peer-reviewed publishing in a journal.  ...is usually a confidential process among a few individuals.  Early-career researchers especially enjoy the...an updated preprint, further increasing their findability. Now, go out there and finish that experiment...10.1371/journal.pbio.3000151 Sever R, Roeder T, Hindle S, Sussman L, Black K-J, Argentine J, Manos W, ...

  7. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    Published
    Nov. 30, 2021, 2:15 p.m.
    ... Five: Share your results.  Does this process remind you of anything? Perhaps the Scientific Method?...expected to write up a report to communicate your findings to your colleagues. So perhaps a better definition...time in a lab learning techniques and taking on independent projects, you will inevitably develop problem...improve. The next time you face a problem, try to be mindful of how you work through it. Ask yourself if you...

  8. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...numerical designations, separated by a slash, that indicate the ITR and serotype, respectively. For example...polarities are rescued from the vector plasmid and individually packaged into the AAV capsids. As a genome conversion...scAAV) vectors promote efficient transduction independently of DNA synthesis." Gene therapy 8.16 (2001)....Elements Resources on Addgene.org Read Our AAV Guide Find AAV Vectors Browse All Viral Vector Resources ...

  9. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...you have to know what resources exist and where to find them! Keeping good records and an organized lab ... are typically more expensive, so you’ll have to find a balance between time savings and budget savings...factors to help you choose the right cloning method Find blog posts about scientific sharing here Download... out our special collection pages to more easily find plasmids Search for a plasmid in Addgene’s repository...

  10. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...some of its dsRNA intermediates into a healthy individual to provoke an immune response and then, hopefully...degrade mRNA transcripts. RNAi activates the RNA-induced silencing complexes (RISCs), which detect dsRNA...dysfunction, it is unclear what the precise mechanism behind the production of these dsRNAs is. A major source...infection. Similarly, if endogenously synthesized dsRNA finds its way outside of the mitochondria, the same inflammatory...

  11. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...sequence information. Collect plasmid information in a binder or digital notebook and make sure you note the ...deposit your plasmids with us, it will be easy to find all of the information needed for the process. Always...primer, you need some space between your primer binding site and the first feature you want to verify because...this region, as outlined above. Avoid primers that bind within or must read through repetitive regions (...

  12. The Future of Research Symposium Boston 2015

    Type
    Blog Post
    Published
    Oct. 20, 2015, 2:30 p.m.
    ... are also beginning to address these issues independently; for example, Harvard’s Therapeutics Graduate... has a compulsory internship to be taken in an industry, regulatory, or clinical environment. It remains...again gather in Boston - this time with the aim of finding solutions to some of the problems that were identified...there for STEM graduates and postdocs in academia, industry and other areas of employment? How many graduates...

  13. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...with a body of literature related to the topic. Finding reliable references and sorting out what they mean...Searching for literature My go-to search engines for finding scientific literature are Google Scholar and PubMed...When embarking on a new literature search, try to find a recent review article to give you an overview ...data. They should explain some of the rationale behind the line of experiments, what data they collected...

  14. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ... developed to treat a multitude of therapeutic indices, including hypertension, cancer, diabetes, cardiovascular...more than 300 different coactivator proteins individually contribute to cell regulation in a coherent ...and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear Receptor Signaling. Dr. McKenna...and Editor-In-Chief of the Open Access MEDLINE-indexed journal Nuclear Receptor Signaling.   Further ...

  15. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

    Type
    Blog Post
    Published
    May 22, 2018, 1:38 p.m.
    ...methionine (i.e. the plasmid complements the deletion). Find the Plasmids Here Plasmid features enable multiple...met deficiencies (manuscript in preparation), indicating that Met protein functions have been largely ... in communicating science to the public. You can find out more about Clare at the Biology Dept. site.  ...Explore Careers at Addgene Resources on Addgene.org Find Yeast Expression Vectors Check Out Our Molecular...

  16. Science communication: 9 Strategies to Get Your Foot in the Door

    Type
    Blog Post
    Published
    Sept. 27, 2017, 1:23 p.m.
    ... new blog post, and live tweet from conferences. Find more tips on using social media as a scientist here...Network – Mingle with pros Where do you meet like-minded science communicators? Attend the annual flagship...started writing three years ago on a field trip in Indonesia. Since then, I have tried many things: photography...Communication Additional Resources on Addgene.org Find Job Openings at Addgene ...

  17. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type
    Blog Post
    Published
    Oct. 6, 2020, 1:15 p.m.
    ...protein variant lacking its endonuclease ability, to bind to genes without editing the genome (Qi et al., ...complementary to the sequence of interest. Upon binding, CRISPRa systems recruit transcription factors ...VP64 fusion protein engineered with aptamers that bind to MS2 proteins. These MS2 proteins then recruit...Resources on Addene.org Read Addgene's CRISPR Guide Find CRISPR plasmids for your research ...

  18. Meet an Addgenie: Insights into Grad School, Career Paths, and More

    Type
    Blog Post
    Published
    June 25, 2020, 1:15 p.m.
    ...during undergrad. Lauren Flynn is the Addgenie behind our "Meet an Addgenie" series. Before heading off...is a more emphasis today about non-academia and industry jobs. Since there are a variety of different career...can make big changes in your career path and still find comfort and happiness there. Read all of the Meet...process, my advisor encouraged me to keep my values in mind as I applied. Thinking about this made me curious...

  19. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ... is a small piece of DNA that can replicate independently from its host’s chromosome. Although we usually...of conjugation, and these are encoded by certain kinds of plasmids — more on that in a minute! Fun fact...antibiotic resistance-mediated selection is indispensable for cloning work, antibiotics and plasmid-encoded...biology, especially when combined with natural inducible expression systems that allow tuning of metabolic...

  20. A Guide to Designing a Scientific Poster: Content, Layout, and More

    Type
    Blog Post
    Published
    Oct. 22, 2019, 12:20 p.m.
    ...poster In almost all scientific posters you will find a title, author names and affiliations, introduction...University. The idea is that the main research finding is written across the center of the poster in a...be cognizant of audience members that are color-blind; avoiding red and green is a good idea for this ... this blog post on navigating the poster session Find tips to make the most of your conference attendance...
Showing: 601 - 620 of 889 results