We narrowed to 776 results for: IRE

...drawbacks limit the utility of these systems: St1Cas9 requires a very specific PAM sequence that limits the number...SaCas9, Zhang’s group used an approach called BLESS (direct in situ breaks labeling, enrichment on streptavidin...

...she had a few minutes to point me in the right direction for my future work. She graciously took me for... Candace Williams, Dave Baltrus, Geof Hannigan, Irene Newton, Juliet Johnston, Kat Milligan-Myhre, Kim...

...process of onboarding a new employee who's coming directly from grad school. The transition has been a bit...thought they would be working in a lab for their entire career. Yet even success in academia means you’...

...as Rep or other initiator proteins, they do not require host proteins for replication and can maintain ...pneumophila, Methylophilus methylotrophus, Myxococcus virescens, M. xanthus, Proteus rettgeri, P. mirabilis, Providencia...

...all of these platforms were developing, Google acquired Youtube and has been growing its online video ..., also known as the science videographer, is a retired botanist and oceanographer who has a blog and several...

...They enjoy the fact that each new challenge will require knowledge of new topics and that they will switch...analyses skills were applicable to my cases. I can directly use my understanding of the molecular behaviors...

...best to start from scratch. Obtain a vial of the desired cell line from a reputable distributor such as ...course of infection. These binding events can be impaired by the electrostatic repulsion of the negatively...

...sequences, which often have absolute position requirements. For example, a KDEL ER retrieval sequence only...for subcellular environment. Currently, she is a SPIRE postdoctoral fellow at the University of North Carolina...

...electron microscopy (cryo-EM) protocols typically require high concentration and purity of the target molecule...particles (eVLPs) offers an option to transduce cells directly with ribonucleoprotein cargo. eVLPs contain no...

...., Huisman, T.M., Verkhusha, V.V. & Hodgson, L. Direct multiplex imaging and optogenetics of Rho GTPases...Piatkevich, K.D. et al. A robotic multidimensional directed evolution approach applied to fluorescent voltage...

...linker. When both orientations are available, a direct comparison of these fusion proteins may reveal ...beta-sheets or alpha helices). An additional requirement is that the site of insertion does not interact...

...forward scatter (FSC) and side scatter (SSC). To acquire these measurements, the cytometer illuminates the... “lymphocyte + single cell + live” population, acquired from the previous gating pipeline, about 53% of...

...to support your work. For example, if your work requires specialized equipment, try finding institutes ... institutes were able to provide the resources required for him to meet his goals. Therefore, he turned...

...antibodies. IPI is working to address this issue directly by creating well characterised antibodies against...solutions that could save both your lab and the entire research enterprise time and funds. It’s time to...

...Similarly, placing UAS upstream of a transgene permits directed expression of that gene in cells that also express...systems, allowing for more complex control over desired gene expression. Take a look at all of the options...

...one since the formation of mEosFP’s chromophore requires a temperature below 30°C and this is not ideal...fluorescence localization data. However, this technique requires harsh fixation conditions to preserve cellular...

...cell that gets the plasmid contains all of the desired gRNAs. This increases the chance that all the edits...editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo Lab multiplexed...

... you to measure the intensity of signal over an entire cycle. Since signals are produced by the presence... practice, multiplexing is quite difficult and requires significant optimization to ensure the results...

...helped us manage our data. To quote Jason Niehaus, Director of Data Science and head of our Data Team, “Our...

...December are eligible to win a: That will warm up the entire lab (hint: you might need some mini marshmallows...