We narrowed to 619 results for: LIS;

1. Tips for CRISPR Gene Editing in Mice

   Type

   Blog Post

   Published

   June 28, 2016, 10:59 a.m.

   ... yourself for potential gRNA sites, or you can utilise several online tools that do it for you. One such...

2. Important Considerations in Optogenetics Behavioral Experiments

   Type

   Blog Post

   Published

   Oct. 1, 2015, 2:30 p.m.

   ...your own neuroscience sub-discipline that has published using the technique and ask if they would be willing...

3. Antibodies 101: Introduction to Immunofluorescence

   Type

   Blog Post

   Published

   Nov. 16, 2021, 2:15 p.m.

   ...principles as other antibody-based assays, like an ELISA or Western Blotting, but it allows you to visualize...

4. Filming Science Videos in the Age of Social Distancing

   Type

   Blog Post

   Published

   May 20, 2021, 1:15 p.m.

   ...audience? As Addgene’s Science Video Production Specialist, I’m constantly thinking about ways to improve...

5. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   Published

   June 17, 2025, 1:15 p.m.

   ... G., Babina, M., Bertin, N., Burroughs, A. M., Carlisle, A. J., Daub, C. O., Detmar, M., Deviatiiarov,...

6. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   Published

   June 27, 2024, 1:15 p.m.

   ...system, as is commonly used in western blotting or ELISAs.  A popular method for indirect labeling employs...

7. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

   Type

   Blog Post

   Published

   Sept. 5, 2017, 1:58 p.m.

   ...HDR), a distinct cellular DNA repair pathway. Accomplishing this usually involves simultaneously introducing...

8. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

   Type

   Blog Post

   Published

   April 24, 2015, 2:39 p.m.

   ....0, with the goal of overcoming the limitations listed above. First, they screened a variety of fluorescent...

9. Antibodies 101: Multiplex Immunofluorescence

   Type

   Blog Post

   Published

   May 23, 2023, 1:15 p.m.

   ... H., Dumitras, C., Gong, B., Nguyen, K., Van Der List, D., Murray, K. D., Engebrecht, J., & Trimmer, J...

10. Plasmids 101: Dimers and Multimers

    Type

    Blog Post

    Published

    Jan. 10, 2023, 2:15 p.m.

    ...forms of colicinogenic factor E-1 from Proteus mirabilis. Biochemistry. 1968 Oct;7(10):3513-20. doi: 10.1021...

11. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    Published

    July 12, 2016, 2:30 p.m.

    ... genome. However, with only 3.4% of founders establishing germline transmission of the transgene, this...

12. Deep Dive: Statistical Tests (Comparisons)

    Type

    Blog Post

    Published

    March 7, 2023, 2:15 p.m.

    ... of comparisons. The Bonferroni does this by establishing a significance threshold at a p value less than...

13. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type

    Blog Post

    Published

    Oct. 8, 2020, 1:15 p.m.

    ...plating, etc. Be wise to choose any of the techniques listed below and always use a second assay to be 100% ...

14. Finding Your Science Policy Path

    Type

    Blog Post

    Published

    Sept. 22, 2020, 1:15 p.m.

    ...connect to your why. I suggest this crowdsourced list of foundational texts as a starting point.  Assess...

15. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type

    Blog Post

    Published

    Oct. 17, 2019, 12:28 p.m.

    ...PMCID: PMC3225502. Boyden, Edward S., et al. "Millisecond-timescale, genetically targeted optical control...

16. When Fidelity Matters: A frank discussion about ligase fidelity

    Type

    Blog Post

    Published

    March 6, 2018, 1:49 p.m.

    ...New England Biolabs® (NEB) researchers recently published a method for the high-throughput profiling of ...

17. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type

    Blog Post

    Published

    June 28, 2018, 3:01 p.m.

    ...doing routine PCR assays. Here is a simple to-do-list to follow when planning epitope tagging experiments...

18. Selecting Your Plasmid Purification Kit

    Type

    Blog Post

    Published

    May 27, 2025, 1:15 p.m.

    ...manufacturers. It also includes a rough comparison of list price between these manufacturers, as price is a...

19. Deep Dive: qPCR

    Type

    Blog Post

    Published

    Sept. 29, 2022, 1:15 p.m.

    ... the forward or the reverse primer.  There is a list of useful primer design resources in the References...