We narrowed to 710 results for: Mak

1. Top Requested Lentivirus and AAV of 2016

   Type

   Blog Post

   Published

   Jan. 6, 2017, 3:56 p.m.

   ...plasmid Cas9 expression is typically cytotoxic, which makes generation of high titer Cas9 lentiviruses challenging...

2. Plasmids 101: Methylation and Restriction Enzymes

   Type

   Blog Post

   Published

   June 30, 2016, 2:30 p.m.

   ...or for diagnostics, we suggest double checking to make sure your results will not be affected by methylation...

3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

   Type

   Blog Post

   Published

   June 2, 2015, 6:06 p.m.

   ...machinery to process gRNAs. Hijacking tRNA processing to make gRNAs In a recently published paper, Yinong Yang...

4. Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

   Type

   Blog Post

   Published

   July 10, 2014, 7:17 p.m.

   ... and devoted 100% of his energy and effort into making the startup self-sustainable. Within a year from...

5. Human Germline Editing Using CRISPR

   Type

   Blog Post

   Published

   Aug. 10, 2017, 2:19 p.m.

   ...differences in editing between iPS cells and embryos make a case for conducting research in embryos should...

6. Choosing the B(right)est Fluorescent Protein: Photostability

   Type

   Blog Post

   Published

   June 8, 2017, 2:30 p.m.

   ...issues with some of the experimental designs used to make these measurements and hope this information can...

7. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ... away. As a result, variable gene expression can make data hard to interpret or can interfere with the...

8. Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools

   Type

   Blog Post

   Published

   April 23, 2018, 2 p.m.

   ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

9. Transferable Skills Guide: Problem Solving

   Type

   Blog Post

   Published

   Nov. 30, 2021, 2:15 p.m.

   ...other skills such as critical thinking, decision making, creativity, or data analysis. Conveniently, these...

10. Transferable Skills Guide: Cross-team Communication

    Type

    Blog Post

    Published

    Nov. 21, 2019, 1:53 p.m.

    ...We took steps towards solving this problem by 1) making sure we qualified these words when we discussed...

11. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

    Type

    Blog Post

    Published

    Oct. 29, 2019, 2:52 p.m.

    ...bioelectrical signals affect pattern memory and decision-making in somatic cell networks. “Developmental bioelectricity...

12. Troubleshooting Your Plasmid Cloning Experiment

    Type

    Blog Post

    Published

    Sept. 24, 2019, 12:55 p.m.

    ...Be mindful of the restriction enzymes you chose. Make sure that enzymes that you are using are not blocked...

13. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

    Type

    Blog Post

    Published

    March 3, 2020, 2:15 p.m.

    ...summaries of recent plasmid deposits and we hope they'll make it easier for you to find and use the plasmids you...

14. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type

    Blog Post

    Published

    Sept. 8, 2020, 2 p.m.

    ... the main source of IL-36 cytokines in the skin making it important to study what is happening within ...

15. Giving gRNAs a Facelift - Synthetic and Beyond

    Type

    Blog Post

    Published

    Oct. 20, 2022, 1:15 p.m.

    ...modifications What is more stable than RNA? DNA! One way to make your gRNA more efficient on a budget is to simply...

16. Pairing CombiGEM and CRISPR for Combinatorial Genetic Screening

    Type

    Blog Post

    Published

    April 12, 2016, 2:30 p.m.

    ... has revolutionized how we decode the genome by making it easy to create specific genetic perturbations...

17. New Tool for Lineage Tracing: The ClonTracer Library

    Type

    Blog Post

    Published

    Sept. 22, 2015, 2:30 p.m.

    ...deposited the library with Addgene because she wanted to make it as easy as possible for researchers to use this...

18. Plasmids 101: Cre-lox

    Type

    Blog Post

    Published

    Jan. 13, 2015, 3:47 p.m.

    ...types, during certain stages of development, or by making the Cre inducible (such as with tamoxifen or doxycycline...

19. Working with Nuclear Receptors

    Type

    Blog Post

    Published

    July 15, 2014, 4:03 p.m.

    ...with a one-stop shop for plasmids and vectors, and making them available to all researchers for a reasonable...

20. Viral Vectors 101: Virus Safety

    Type

    Blog Post

    Published

    June 22, 2023, 1:15 p.m.

    ...having an ‘off’ day – you are both more likely to make a mistake and more likely to want to cut a few corners...