We narrowed to 867 results for: PLE;

...YFP) to the new and improved fruit colors (e.g., apple, papaya, and tomato) and the photoactivatable fluors...quickly. Researchers should keep in mind that a very simple sequential or double restriction enzyme digest ...on the corresponding plasmid pages (e.g. see Supplemental Documents here). If you’re not sure where your...

...observe what’s happening inside cells for at least a couple of decades. Green is the classic color, but fluorescent...signal-to-background ratio, thus, allowing higher resolution in samples containing substantial autofluorescence background...direction of biosensors capable of revealing, for example, how muscles work inside a living animal. For those...

...recognizes its folds, especially the repeat:antirepeat duplex. Cas12 recognizes the 2’-OH of RNA in this region...sequence reduces the stability of the genome-bound complex. This decreases the Cas protein’s tolerance for...on-target efficacy. For perfect sequence matches, the complex is stable enough that the DNA substitution doesn...

...helps improve your interpersonal relationships with people you’re collaborating with remotely. Depending on...as Slack. Share data and documents While having multiple scientists edit the same document may sound like...collaborators. Being able to present data from multiple aspects of a collaboration and immediately discuss...

...kind of walking them through the steps of, for example, cutting open a heart to look at the inside. We...a lot of interactives and videos, as well, to supplement the hands-on [aspect of the labs],” says Caitlin...new technologies in education and ask ‘Can we implement that?’ [And think about] where we [eScience] are...

...Iowa in the Department of Pharmacology, I taught a couple of lectures in the undergraduate pharmacology course... editing sphere.” But things aren’t always that simple, especially when your spouse is also in science... stumbled upon eScience by luck, by chance, by complete happenstance. It was on Indeed or one of the job...

...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and ...Cpf1: A New Tool for CRISPR Genome Editing and Multiplex Genome Editing with CRISPR-Cpf1 Day 4: Edit validation...

...University of Akron, Ohio. Congratulations! There was plenty of geeky fun to be had at ASM Microbe as well as...science theme, the Artwalk on the exhibit floor had plenty of wonderfully nerdy art for sale, and photos of...presented at the conference, it looks like there are plenty more exciting plasmid tools on the horizon for ...

...skills help when it comes to finding a job. Many people in science will work in industry or take on collaborative...and their interest in science more generally to people outside of academia. Here are my recommendations... they start asking you questions. Remember that people like a good story and science involves characters...

...incorporate into their own labs. This week we are pleased to announce the submission of the results of our...be examined. Increased yields with sucrose supplementation is serotype specific In our study we observed...preparations for, we wanted to be certain that this supplementation strategy would have no effect on the purity...

...ever like to write about a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids...APOBEC3A-Cas9n-UGI and APOBEC3B-Cas9n-UGI base editing complexes are more efficient than the BE3 plasmid containing...exhibiting its versatile utility in quantifying multiple gene editing activities. Click here to find the...

...an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ... like as long as it’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas...also drive different levels of expression. For example, CAG is a strong promoter that drives very high...

...Balazs. “By combining those with [sera] we got from people that have been given the vaccine, we've been able...used live virus (Cele et al., 2021). “We weren't completely shocked [with these results] because we were ... AG, Iafrate AJ, Naranbhai V, Balazs AB (2021) Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced...

...manipulated with mutations in the replicon. For example, the pMB1 ori maintains about 20 copies per cell... any questions? Need something clarified? If so please, post in the comments.  References Chatterjee A...Schultz PG. A versatile platform for single- and multiple-unnatural amino acid mutagenesis in Escherichia...

...common lexicon for genome editing studies. These complementary goals are aimed at improving translation of ... loosely paraphrased in the tweet below. The complexity and challenges of homology directed repair (HDR...editing and low off-target editing rates across multiple systems. Read our introductory blog post, then...

...jGCaMP7 sensors with different properties (for example, overall brightness or on/off kinetics) that makes...quantify targeting of particular cell types. For example, the mDlx enhancer restricts expression to GABAergic... particular catalog item is available for order please email [email protected] with the vector name and...

...many different things in parallel.” Alternating Hemiplegia of Childhood Melodi McSweeney (right), a graduate...responsible for the disease known as Alternating Hemiplegia of Childhood (AHC). Patients affected by AHC ...from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful and accompanied by migraines...

...discussion. The Forced Choice Analysis is a fun example and you can find it in our Mentoring for Scientists...help the Mentee feel a sense of accountability to complete action items and make progress. To illustrate ...with regular accountability checks. Focus on implementation of your goals and respect the time your Mentor...

...ever like to write about a recent plasmid deposit please sign up here.        A retroviral mouse CRISPR...Thus, the bEFPs bind the UAS via the dCas9-gRNA complex which allows transcription from the promoter.  ...should serve as both a powerful tool for generating complex regulatory systems and further the study of natural...

... It doesn’t have to be this way. We can never completely eliminate the risk that a minor variation in ...collective priority list. There has been progress—for example, a number of funders (e.g., the NIH) now look for...Press.  She trained originally in cell biology, completing her PhD at the MRC LMB in Cambridge UK and then...