We narrowed to 860 results for: PLE;

...Iowa in the Department of Pharmacology, I taught a couple of lectures in the undergraduate pharmacology course... editing sphere.” But things aren’t always that simple, especially when your spouse is also in science... stumbled upon eScience by luck, by chance, by complete happenstance. It was on Indeed or one of the job...

...University of Akron, Ohio. Congratulations! There was plenty of geeky fun to be had at ASM Microbe as well as...science theme, the Artwalk on the exhibit floor had plenty of wonderfully nerdy art for sale, and photos of...presented at the conference, it looks like there are plenty more exciting plasmid tools on the horizon for ...

...CRISPR Antimicrobials Day 7: Multiplexing and Cas9 variants: CRISPR 101: Multiplex Expression of gRNAs and ...Cpf1: A New Tool for CRISPR Genome Editing and Multiplex Genome Editing with CRISPR-Cpf1 Day 4: Edit validation...

...skills help when it comes to finding a job. Many people in science will work in industry or take on collaborative...and their interest in science more generally to people outside of academia. Here are my recommendations... they start asking you questions. Remember that people like a good story and science involves characters...

...ever like to write about a recent plasmid deposit please sign up here.   Listen this episode of Hot Plasmids...APOBEC3A-Cas9n-UGI and APOBEC3B-Cas9n-UGI base editing complexes are more efficient than the BE3 plasmid containing...exhibiting its versatile utility in quantifying multiple gene editing activities. Click here to find the...

...common lexicon for genome editing studies. These complementary goals are aimed at improving translation of ... loosely paraphrased in the tweet below. The complexity and challenges of homology directed repair (HDR...editing and low off-target editing rates across multiple systems. Read our introductory blog post, then...

...manipulated with mutations in the replicon. For example, the pMB1 ori maintains about 20 copies per cell... any questions? Need something clarified? If so please, post in the comments.  References Chatterjee A...Schultz PG. A versatile platform for single- and multiple-unnatural amino acid mutagenesis in Escherichia...

...an ITR during routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ... like as long as it’s ~4.4 kb or smaller. Some examples include a reporter like GFP, some smaller CRISPR-Cas...also drive different levels of expression. For example, CAG is a strong promoter that drives very high...

...jGCaMP7 sensors with different properties (for example, overall brightness or on/off kinetics) that makes...quantify targeting of particular cell types. For example, the mDlx enhancer restricts expression to GABAergic... particular catalog item is available for order please email [email protected] with the vector name and...

...incorporate into their own labs. This week we are pleased to announce the submission of the results of our...be examined. Increased yields with sucrose supplementation is serotype specific In our study we observed...preparations for, we wanted to be certain that this supplementation strategy would have no effect on the purity...

...Balazs. “By combining those with [sera] we got from people that have been given the vaccine, we've been able...used live virus (Cele et al., 2021). “We weren't completely shocked [with these results] because we were ... AG, Iafrate AJ, Naranbhai V, Balazs AB (2021) Multiple SARS-CoV-2 variants escape neutralization by vaccine-induced...

...many different things in parallel.” Alternating Hemiplegia of Childhood Melodi McSweeney (right), a graduate...responsible for the disease known as Alternating Hemiplegia of Childhood (AHC). Patients affected by AHC ...from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful and accompanied by migraines...

...discussion. The Forced Choice Analysis is a fun example and you can find it in our Mentoring for Scientists...help the Mentee feel a sense of accountability to complete action items and make progress. To illustrate ...with regular accountability checks. Focus on implementation of your goals and respect the time your Mentor...

...about FPs than is currently represented at fpvis (complete spectral information, accession numbers, sequences...the goal of encouraging user contributions (using simple web forms) while also maintaining a relatively ...in the early stages of development, and there is plenty of room for improvement and elaboration.  Current...

... SARS-CoV-2 interacts with human proteins. For example, we used HEK293T cells in our first study, but ...collaborative science, and making it accessible to all people, not just scientists! *If you are requesting plasmids...plasmids for coronavirus or COVID-19 research, please email Addgene at [email protected] with your order...

... It doesn’t have to be this way. We can never completely eliminate the risk that a minor variation in ...collective priority list. There has been progress—for example, a number of funders (e.g., the NIH) now look for...Press.  She trained originally in cell biology, completing her PhD at the MRC LMB in Cambridge UK and then...

...ever like to write about a recent plasmid deposit please sign up here.        New toolkit for N or C terminal...binds to TIR1 of the SCF-TIR1 ubiquitin ligase complex, promoting its interaction with the degron sequence...used ChRs in that they cause a rapid and almost complete desensitization when exposed to continuous bright...

...size 20 µL samples of control AAV that scientists can use for serotype testing. These samples are available... particular catalog item is available for order please email [email protected] with the vector name and...

...produced? Purifying exo-AAVs is actually a bit simpler than the classical purification protocol involving...have tried to modify it to improve yields. For example, transfecting the producer cell line with an expression...were always normalized to their genomic titers). Examples include the use of exo-AAV1 for transducing sensory...

...techniques are continually being refined; it is completely plausible that scientists will eventually have...many things we can navel-gaze over from the last couple of weeks, including whether or not scientists can...history of medical research, there have always been people who unethically experiment on humans, and they ...