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Showing: 601 - 620 of 835 results

  1. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    ...save you the time that would be spent making and testing entirely new gRNA designs. You can now easily find...variations (e.g. SNPs) exist. Additionally, consider testing multiple gRNAs targeted to the same locus to ensure...

  3. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ... FPs since its emission wavelength (612 nm) is distinct from other commonly used FPs, and GFP does not...localization that have been encountered by some investigators when using forms of mEos." The ability to repurpose...

  4. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    Published
    June 19, 2015, 3:08 p.m.
    ..., designed for use in B. subtilis, contains the MISTIC (membrane-integrating sequence for translation ...integral-membrane protein constructs) protein tag. MISTIC drags tagged proteins into the membrane and may...

  5. CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]

    Type
    Blog Post
    Published
    Feb. 18, 2015, 3:09 p.m.
    ... inferred based on correlative biochemical characteristics and ectopic reporter assays. We have been interested...Bauer is a pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical...

  6. Celebrating Accomplishments in the Lab

    Type
    Blog Post
    Published
    May 8, 2014, 3:07 p.m.
    ...? This final post in the PI Interview Series investigates how these three PIs reward their team and manage...during the child-rearing years, learning “lots of interesting things from children, their classmates, and parents...

  7. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    Published
    Jan. 2, 2014, 3:44 p.m.
    ...frequently used pages in your notebooks and go wild with sticky notes and labels. Color code your Post-its and ...write brief descriptions on each. Place the notes sticking up out of the top of the notebooks, so that you...

  8. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    Published
    Nov. 19, 2013, 2:37 p.m.
    ...localization of individual proteins, but also investigate protein-protein interactions via FRET. Sidae ...partial gene deletion, overexpression (by promoter substitution), and tagging at either the N- or C-terminus...

  9. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...resources this way, the CCRP2 is giving Rutgers investigators the agility to rapidly initiate complex research... As researchers around the world continue to investigate COVID-19, and once universities reopen and restart...

  10. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...months of strategizing, designing, developing, testing - and repeat - we’re proud and excited to finally... than through the ‘front door,’ the homepage is still important. It needs to express value, clearly communicate...

  11. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    Published
    May 12, 2015, 1:32 p.m.
    ...recombination at pseudo- or cryptic loxP sites (estimated to occur at a frequency of 1.2 per megabase in...toxicity of this enzyme in Drosophila. If you're still reading this post and you don't work in Drosophila...

  13. Build Your CRISPR Vocabulary

    Type
    Blog Post
    Published
    July 2, 2024, 1:15 p.m.
    ... but boy does it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is ...appears between each spacer in the CRISPR array, distinguishing the foreign sequences and keeping the array...

  15. Antibodies 101: Fc Effector Functions

    Type
    Blog Post
    Published
    May 18, 2023, 1:15 p.m.
    ...process known as phagocytosis.  Other functions We’re still learning about Fc effector functions and all that...driving particular cells or responses. And there are still antibody isotypes and Fc receptors that we don’t...

  16. CRISPR 101: Drug discovery and CRISPR

    Type
    Blog Post
    Published
    May 4, 2023, 1 p.m.
    ...sometimes even genetic pathways that are worth investigating for drug development. Due to the difficulty ... to determine if a patient would respond to an existing therapy. In theory, CRISPR could even be used ...

  17. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...for new ways to serve the research community. Questions? Feel free to reach out to us on Twitter, Facebook... Ishrat, Jane, Jason, Jessica, Joel, Jonathan, Justine, Karen, Kate, Kayla, Kelly, Kory, Kris, Leila, ...

  19. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ...two gRNAs.  For nickase applications, a common question is: how should the gRNAs be oriented in comparison...insertions. These profiles are likely due to the distinct overhang patterns generated by the nickases; D10A...
Showing: 601 - 620 of 835 results