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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 863 results for: TOR;

Showing: 601 - 620 of 863 results

  1. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    Published
    Dec. 2, 2019, 2 p.m.
    ...Split-BioID: A conditional proteomics approach to monitor protein-protein interactions Split-BioID combines... a split BirA* molecule that can reassemble to restore activity when fused to two interacting proteins... in one experiment a scientist can confirm and monitor the interaction of two proteins while also biotin...Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally defined protein...

  2. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    Published
    April 9, 2019, 1:13 p.m.
    ... green fluorescent protein (GFP) from Aequorea victoria and its variants are genetically encoded fluorescent... of fluorescent proteins derived from Aequorea victoria GFP sticks out and can be considered a linker ...Janetopoulos, Chris, Tian Jin, and Peter Devreotes. "Receptor-mediated activation of heterotrimeric G-proteins...et al. "Expanded dynamic range of fluorescent indicators for Ca2+ by circularly permuted yellow fluorescent...

  3. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ...have previously used histone deacetylase (HDAC) inhibitors to increase transcription of the integrated HIV...turned to the CRISPR/Cas9 Synergistic Activation Mediators (SAM) system available from Addgene. This system...TALENs focused primarily on disrupting CCR5, the receptor that HIV-1 uses to enter a cell. ZFNs and CRISPR...W. CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust ...

  4. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...reduced by adding a protease inhibitor cocktail and phosphatase inhibitors to your lysis buffer. Additionally...ladder runs off the gel. Luckily, this is easily monitored in real time, as the dye front (and many ladders...resources with examples, like BioRad’s Western Blot Doctor page, or robust discussions, like ResearchGate’...longer time Protein degradation Add protease inhibitors to lysate Insufficient denaturing conditions...

  5. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...to sort cells based on the expression of a new receptor that is of interest to you. You found a couple...may not be the most accessible option for many laboratories. Additionally, it can be difficult to distinguish...understand if protein:protein interactions could be a factor in your analysis. Example: You are planning an ...Koch-Nolte F, Pascual N, Rodríguez-Barbosa J-I, Torensma R, Turley H, Pulford K, Banham AH (2015) The European...

  6. Welcome to the Lab Bench! New Protocol Series Helps Trainees Get Started in the Lab

    Type
    Blog Post
    Published
    Sept. 7, 2021, 1:15 p.m.
    ...protocols for plasmid cloning and working with viral vectors, but what we lacked were videos guiding completely...completely new members of the lab or those taking introductory lab courses. The Intro to the Lab Bench series... need a refresher on some best practices for introductory science techniques. It’s also great for teachers...

  7. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...improve its stability. Additionally, the choice of vector can impact the expression level of an antibody ...lesional skin from a psoriasis patient, showing an inflammatory phenotype. Each protein was detected using an... cellular pathways by binding to cell surface receptors. These applications are typically used for investigating...Arbabi-Ghahroudi M (2017) Camelid Single-Domain Antibodies: Historical Perspective and Future Outlook. Front Immunol...

  8. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...fields.  Addgene is a global nonprofit plasmid repository. Over 2,000 labs have deposited plasmids to Addgene...Zhang acknowledge the role of data and materials repositories in aiding the dramatic spread of the Cas9 technology...accelerated through a combination of open-source distributors such as Addgene, as well as a number of online...

  10. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...any applications. DNA quantity is fairly self explanatory and achieved by performing different sized preps...about!) are a significant source of waste in laboratories. To help combat this, some manufacturers have...Minimized amount of silica ACT environmental impact factor label Non-silica based, avoids use of chaotropic...kit for your lab is dependent on many different factors as we detailed above, so be sure to pick what kit...

  11. Celebrating the 2024 Nobel Prize in Chemistry

    Type
    Blog Post
    Published
    Oct. 17, 2024, 1:15 p.m.
    ... fans of all our depositors here at Addgene — your contributions make the repository what it is. So we...we’re thrilled this week to congratulate depositor David Baker, who was awarded the Nobel Prize in Chemistry...

  12. Top Requested Plasmid of 2017 - pMD2.G

    Type
    Blog Post
    Published
    Jan. 10, 2018, 4:13 p.m.
    ...2016 were CRISPR plasmids), but so are lentiviral vectors, many of which are used to deliver Cas9 and other....G from Didier Trono’s lab! Lentivirus envelope vector pMD2.G This plasmid has been requested nearly 6,000... our Viral Production Process Find other Viral Vectors ...

  13. Tips for Working in Industry & Success in Management: Interview with Crystal Shih

    Type
    Blog Post
    Published
    Oct. 5, 2017, 1:13 p.m.
    ...Crystal Shih is an Investigator III at Novartis, a multinational pharmaceutical company based in Switzerland...'ve helped her at Novartis. The importance of mentorship in developing career skills. Outro Many thanks...Addgene Find more management resources in our Mentoring for Scientists Series Learn about Turning Your...

  14. Addgene Stands with the Scientific Community

    Type
    Blog Post
    Published
    April 23, 2025, 1:30 p.m.
    ...resources: Preserving scientific assets: We hold a repository of valuable research materials that might otherwise...continue to offer high-quality DNA sequencing, viral vector preparations, and antibody production that labs...continue.  Moving Forward Together Throughout history, the scientific community has demonstrated remarkable...

  15. A Scientist's Guide to Ebola

    Type
    Blog Post
    Published
    Oct. 16, 2014, 3:01 p.m.
    ...the virus itself that kills you, but the cytokine storm it ultimately sets off.  The virus works its way...cells and turn the cell into a veritable virus factory.  Their findings were reported in the Journal of...and the target of neutralizing antibodies and inhibitors of attachment and fusion. Recommended Reading...

  16. Addgene's Website Update

    Type
    Blog Post
    Published
    April 8, 2024, 2:15 p.m.
    ...two options: “Search Repository” and “Deposit Materials”. The “Search Repository” function is our same...section: Tools of the Day. Our plasmid and viral vector of the day have a fresh new look and a fresh new...

  17. How Do I Place an Addgene Order Using a PO Number?

    Type
    Blog Post
    Published
    Feb. 27, 2020, 2:15 p.m.
    ...'Regan Ordering jeans online from your favorite store is easy. Ordering genes on plasmids online is a ...nonprofit, Addgene does not own the material in our repository. We distribute it on behalf of depositing institutions...full of information about how to deposit plasmids, store your plasmids or viral items, technical support,...

  18. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    Published
    Dec. 31, 2019, 2:26 p.m.
    ...Anti-CRISPR, 2in1 Cloning, Fluorescent Voltage Indicators, and Photoswitchable Proteins by Cary Valley,...Academic Job Interview by Erik Snapp Addgene depositor and guest blogger Erik Snapp breaks down how to... blog posts Resources at Addgene Browse the repository Find educational resources at Addgene Search ...

  19. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    Published
    Dec. 19, 2013, 3:46 p.m.
    ..., even when all kinds of other factors, including journal impact factor, study topic, and the corresponding...preference to journal access granted via a public repository or authors’ own web sites. 9)      The year 2013...

  20. A new platform for fast functional characterization of potentially cancerous mutations

    Type
    Blog Post
    Published
    May 7, 2019, 12:39 p.m.
    ...MCF10A cellular models. Ba/F3 and MCF10A are growth factor dependent cell lines and any mutations that that...advantages will be revealed in the absence of growth factor. The team assigned the mutations to functional ...significantly increased cell viability, and these “activators” may be the direct drivers of tumor formation...
Showing: 601 - 620 of 863 results