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We narrowed to 806 results for: Tes

Showing: 601 - 620 of 806 results

  1. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...idea of the basics of immunoimaging. A scientist conjugates an antibody with a signaling molecule, the antibody...secondary antibodies on hand and use them to easily test different antibodies or different signaling molecules...

  2. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    Published
    July 14, 2021, 1:15 p.m.
    ...as KLH or Freund’s adjuvant is injected, which stimulates the overall immune response. Once the initial...weeks to increase the antibody titer. The titer is tested following each immunization until the desired level...

  4. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    Published
    May 30, 2023, 1:15 p.m.
    ...express genes or gene sets over 4.7 kb. Co-infection rates for AAVs are generally high enough for this approach... guest blogger Didem Goz Ayturk with edits and updates from Addgenies Karen Guerin and Susanna Stroik....

  5. The time and cost required to make a plasmid

    Type
    Blog Post
    Published
    March 23, 2023, 1:15 p.m.
    ...reagents and consumables, especially for things like plates and gels, which either don’t scale or have limited...my ability to tell. But even from these rough estimates, it’s clear that community engagement with and...

  6. New Tool for Lineage Tracing: The ClonTracer Library

    Type
    Blog Post
    Published
    Sept. 22, 2015, 2:30 p.m.
    ...below): 1. Resistant cells among the separate replicates would contain the same labels - i.e. they would...subpopulation 2. Resistant cells among the separate replicates would have different labels - i.e. they would...

  8. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...immunogenicity of AdVs is what makes them ideal candidates for applications in oncolysis and vaccination...by recombination. It is therefore recommended to test for the presence of RCA in the viral stock by doing...

  10. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ... with plasmids that allow them to eat unusual substrates that other organisms can’t use. Other plasmids...undiscovered functions, many are simply genetic parasites. Cryptic plasmids tend to be small and poorly ...

  11. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...oxygenase for chromophore formation (abundant in eukaryotes) A Near-Infrared BiFC Reporter for In Vivo Imaging...oxygenase for chromophore formation (abundant in eukaryotes) Bright monomeric near-infrared fluorescent proteins...

  12. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...19, aside from the widely used qPCR and antigen tests.  Labs including Jennifer Doudna’s lab and Feng ...colorimetric readout that can be completed in just 45 minutes. The team deposited the reverse transcriptase, ...

  13. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...prohibitively expensive for many labs. We’ve created and tested plasmids that use CRISPR/Cas9 to endogenously tag... few single base pair mutations in crRNA target sites found within the homology areas of the plasmid to...

  14. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    Published
    June 22, 2023, 1:15 p.m.
    ...transfer plasmid, the component that physically integrates into the host genome, the virus is replication...decontaminating liquid waste, remember that the percentage indicates the proportion of the decontaminate in the waste...

  15. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    Published
    April 6, 2023, 3 p.m.
    ...clones and thus the potential to bind many different sites on the target protein they tend to have a strong...and bind to your primary antibody. Manning et al. tested a variety of commercially available secondary antibodies...

  16. A Look at Addgene's QC Process

    Type
    Blog Post
    Published
    May 6, 2025, 1:15 p.m.
    ...the deposited sequence(s) in SnapGene. SnapGene annotates the plasmid maps based on SnapGene’s “Common Feature...at a best practices meeting, where the QC team updates and reviews our sequencing QC standards to ensure...

  17. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...number of messages in our thread or remove a good 5 minutes from our phone call. If your concern is based...get. There are questions that are answerable in minutes and questions that could take weeks to resolve ...

  18. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...virus in Brazil that was correlated with greater rates of infection and rare, but severe, symptoms, including...unlike most research, but the urgent need for the test did catalyze our resolve to get the work done as...

  19. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...0.22 µm filter) or by a centrifugation step (5 minutes at 300-500 g). After removing packaging cell debris...fibronectin-enhanced retroviral transduction of primary human T lymphocytes for adoptive immunotherapy." Acute Leukemias ...

  20. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...biology of the worm into a computer model that recapitulates how molecular biology impacts worm locomotion...generally funded on a membership model - everyone contributes each month - with only occasional supplementation...
Showing: 601 - 620 of 806 results