We narrowed to 643 results for: Vars
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TypeBlog PostPublishedMay 3, 2016, 2:30 p.m.... based on the well-studied T7 phage, to target various types of bacteria. In mixed populations, their ...
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Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m....between column runs. The TEV protease itself, with various mutations used to increase its stability activity... -
Save Time with Transient Plant Leaf Transformations
TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m....simple and easy technique to implement. Success might vary between experimenters. Vacuum infiltration (Mortensen... -
What's Your Organism? Expanding Genomic Tools via the NSF EDGE Program
TypeBlog PostPublishedMay 2, 2019, 12:41 p.m....manipulation. The ReMOT control system (Receptor-Mediated Ovary Transduction of Cargo) delivers Cas9 RNP to the... -
Bioinformatics at Addgene
TypeBlog PostPublishedJuly 14, 2022, 1:15 p.m....re-use or borrow existing code and control how the various pieces were connected, as this enabled us to rapidly... -
New CRISPR Tools: Cas7-11 and PASTE
TypeBlog PostPublishedFeb. 17, 2022, 2 p.m....then used this information to identify a large variety of related bacteria with similar Cas systems. Some... -
Bacterial Toxin-antitoxin Systems as Molecular Biology Tools
TypeBlog PostPublishedNov. 1, 2018, 12:35 p.m....bacteria. They consist of a toxin which can affect a variety of cellular processes and an antitoxin that suppresses... -
Neuronal labeling with Spaghetti Monster
TypeBlog PostPublishedAug. 14, 2018, 12:42 p.m....detection: fluorescent protein structure and color variants." Cold Spring Harbor Protocols 2009.12 (2009):... -
QC Sequencing Technologies at Addgene
TypeBlog PostPublishedJuly 15, 2025, 1:15 p.m....Addgene have minor differences due to in silico variations and lack of verification. To address this issue... -
Plasmids 101: Shuttle Vectors
TypeBlog PostPublishedMay 20, 2025, 1:15 p.m....eukaryotes. But be careful — antibiotic mechanisms vary! Aminoglycoside antibiotics also block protein synthesis... -
New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression
TypeBlog PostPublishedJan. 24, 2023, 2:15 p.m....ended up being very straightforward to engineer various cell lines from different tissues and mammalian... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....Reference References del Solar G, Giraldo R, Ruiz-Echevarría MJ, Espinosa M, Díaz-Orejas R (1998) Replication... -
Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone
TypeBlog PostPublishedFeb. 18, 2020, 2:15 p.m....the Mentor-Singleton relationship. Some of the variability was organic, as some people need less assistance... -
Viral Production at Addgene
TypeBlog PostPublishedOct. 24, 2023, 1:15 p.m....control (QC). The specific QC experiments performed varies for each viral lot. To learn which specific QC ... -
Viral Vectors 101: Pseudotyping
TypeBlog PostPublishedApril 20, 2021, 2:30 p.m....cytotoxicity and the mechanisms behind the cytotoxicity can vary. For example, the popular VSV-G envelope protein... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog PostPublishedApril 2, 2019, 1:44 p.m....some of our Addgenies had an idea: sqh mutant fly ovaries had “squiggly” actin fibers that resemble spaghetti... -
Supporting Reproducibility with a Connected ELN
TypeBlog PostPublishedJan. 17, 2019, 1:38 p.m....document is edited, and it’s possible to link between various documents. A second example is the integration ... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m....packages needed to travel meant the accessibility varied country to country. Figure 1: Number of countries... -
Tips for Writing a Good Cover Letter
TypeBlog PostPublishedMay 21, 2024, 1:30 p.m....application. Even within an organization, there can be variance. At Addgene, our content team, where writing is... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....featured topic page xCas9: Engineering a CRISPR Variant with Pam Flexibility RNA Editing with Cas13 and...