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We narrowed to 907 results for: addgene

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Showing: 601 - 620 of 907 results

  1. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...available through Addgene. This means you could perform a screen with one library from Addgene, and then use...PMCID: PMC5292764. Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design ...melanogaster, and T. gondii are available through www.addgene.org/crispr/libraries/. Additional libraries are...gRNA Tips for a 1st CRISPR User Resources on Addgene.org Learn More About CRISPR Find CRISPR Screening...

  2. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...tissues. Subscribe to Viral Vector Blog Posts from Addgene 1. Location, location, location! What do buying...populations. Find Ready-to-Use Retrograde AAV Preps at Addgene 2) Comparison of genetically engineered receptors...PubMed PMID: 28073291. Additional Resources on the Addgene Blog AAV: A Versatile Viral Tool for Gene Expression..., AAV7, AAV8, AAV9 Adapted from https://www.addgene.org/viral-vectors/aav/aav-guide. Where in the tissue...Viral Vector Elements Additional Resources on Addgene.org Find AAV Vectors for Your Research Get Ready-to-Use...

  3. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ... What is an Antibody? Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies...different research applications they can be used for. Addgene’s Antibody Plasmid Collection includes plasmids ...10.3389/fcimb.2013.00072 Additional resources on the Addgene blog: Read about another reagent sharing platform...

  4. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...AAV plasmids containing the active promoters at Addgene. Performance of synthetic promoter AAVs in mice... other tissues.  Additional resources on the Addgene blog Read more about using AAV for retinal gene...of our viral vector blog posts Resources at Addgene.org Find AAV plasmids Find ready-to-use AAV viral...

  5. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...an extensive library of monoclonal antibodies; Addgene collaborates with these companies to create and...10.1371/journal.pbio.1002165 More resources on the Addgene blog Antibodies 101: ValidationSelecting the Right...

  6. Simple CRISPR-based Epigenetic Editing: dCas9-directed DNA Demethylation

    Type
    Blog Post
    Published
    Dec. 14, 2021, 2:15 p.m.
    ...that it may later be excised by Cre recombinase. Addgene has several great options: pLV hUbC-dCas9-T2A-GFP...References and resources:  Additional Resources on the Addgene blog CRISPR101: Editing the Epigenome  Mapping...the 4D Nucleome with CRISPR/Cas9 Resources at Addgene.org Read our CRISPR Guide to Epigenetics References...

  8. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ... using Nanoblades? Plasmids are available from Addgene. Genome editing primary cells with Nanoblades Primary...PMCID: PMC 6318322. Additional resources on the Addgene blog Learn about RNP delivery Learn about CRISPR...Browse our CRISPR 101 blog post Resources on Addgene.org Check out our comprehensive CRISPR guide Find...

  9. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...name a few.   Find Many FRET-Based Biosensors on Addgene's Biosensor Pages   Recent innovations have demonstrated...PubMed PMID: 26210398. Additional Resources on the Addgene Blog Tips for using FRET in Your Experiments Learn...FRET Based Biosensors Additional Resources on Addgene.org Find FRET Pairs Check Out Fluorescent Protein...

  10. dTAG - You're it!

    Type
    Blog Post
    Published
    June 21, 2018, 2:06 p.m.
    ...generated a series of plasmids that are available from Addgene. These plasmids enable lentiviral expression of...molecule: dTAG-47 Additional Resources on the Addgene Blog Easi-CRISPR: Generating Knock-In and Conditional...endogenous gene tagging in human cells Resources on Addgene.org Browse all CRISPR plasmids CRISPR plasmids for...

  11. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ... use. Alyssa D. Cecchetelli is a Scientist at Addgene. She received her PhD from Northeastern University...proteins in plant root exudates Resources on the Addgene Blog Learn about inducible promoters Learn about...promoters Check out protein tags Resources on Addgene.org Bacterial expression systems Search for plasmids...

  13. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 1, 2018, 1:57 p.m.
    ...with your research? Plasmids are available from Addgene! References Schopp, I.M., Ramirez, C.C., Debeljak...Central PMCID: 5053326. Additional Resources on the Addgene Blog Learn about techniques for visualizing protein...-DNA Interactions with CUT&RUN Resources at Addgene.org Find plasmids from the Bethune Lab Find Plasmids...

  14. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...Resources on Addgene.org Addgene’s CRISPR Guide CRISPR Plasmids and Resources Resources on the Addgene blog ...

  15. Antibodies 101: Conventional vs Spectral Flow Cytometry

    Type
    Blog Post
    Published
    Aug. 3, 2023, 1:15 p.m.
    ...and Conjugates Resources on Addgene.org Addgene's Antibody GuideAddgene's collection of ready-to-use recombinant...early life.    Resources More resources on the Addgene blog Antibodies 101:Selecting the Right AntibodyAntibodies...

  16. Plasmids 101: 5 factors to help you choose the right cloning method

    Type
    Blog Post
    Published
    Aug. 21, 2018, 12:31 p.m.
    ...be sure to deposit your finished constructs at Addgene!). References 1. Li, Chaokun, et al. "FastCloning...PMCID: PMC3207894.  Additional Resources on the Addgene Blog Read other Plasmids 101 blog posts New to...Download the Plasmids 101 eBook Resources on Addgene.org0 Check out protocols for plasmid cloning Find...

  17. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ... why they should give you money. Check out the Addgene Blog for Some Ways to Improve Your Science Communication...multiplex assays.   Additional Resources on the Addgene Blog Learn How to Become a Grant Writing Professional...

  20. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...acquire during their PhD and postdoctoral training. Addgene executive director Joanne Kamens has written a ...Further Reading Find more Career articles on the Addgene Blog Read Joanne Kamen's Management for Scientists...a 5-part blog on the topic:  http://blog.addgene.org/management-for-scientists-managing-vs-leading. The...
Showing: 601 - 620 of 907 results