...for a lentiviral vector to be used in mammalian cells, check Lentiviral and Mammalian Expression). This...

...check its subcellular location and solubility. If it’s membrane-bound or located in a subcellular compartment...reagent. Be aware that this approach requires excellent attention to detail, since you’ll end up with ...question before running one.  Good on gradients? Excellent! Now, let’s head west, intrepid reader! Protein...strengths. If that’s not working, consider using a subcellular fractionation kit or a lysing protocol developed...to see if one works better than another. For nitrocellose membranes, you can even give gelatin a shot....strength Sonication based lysing protocol Subcellular fractional kit or lysing protocol Not enough...

...expecting a salary increase have had that increase cancelled. Rounding out the remainder, 2% of postdocs who... are continuing with their plans, and 17 have cancelled their plans. 2 of those raising - the University...Illinois and the University of Michigan - initially cancelled their plans, but have since reversed their position... In addition, PIs at some of the institutions cancelling plans are going ahead with raises anyway, including...injunction, as we started to hear of institutions cancelling plans to raise salaries, the only postdocs we...

...teams continue to keep up with the demand with excellence and dedication. We don’t have worry or wonder...right? Upgrades to the internal LIMS pages and excellent work on our external website appearance and functionality...support. The Addgene management team leads with excellence. They are one of the main reasons I have so few...Addgenies. I never worry about their dedication, excellent intentions, or incredible drive to succeed.  Thanks...

...chimeric cellulases that enable E. coli and Citrobacter freundii to generate sugars from cellulosic biomass...inspire new Ghanaian scientists to continue to do excellent work in the biological sciences. Future awardees...

...transferable skills while you are also learning to be an excellent scientist. Leadership and management Take a lead...supportive editors so your first drafts are always excellent Technical knowledge and creativity Become a “...local networking and trade organizations Another excellent perspective on this topic can be found on the ...

...give you a chance at an interview:  1) Have an excellent resume ready to be submitted The structure and...successful application. It takes time to prepare an excellent resume…it is worth it. Read here to get help if...if you are having trouble preparing your most excellent resume. 2) Follow job boards and check them regularly...

...is an excellent resource that has gained a lot of traction with researchers. It is an excellent way to...programs and departments Graduate school was an excellent time to try new things, develop new skills, and...

...Fluorescent Protein Plasmids 101: Visualizing Subcellular Structures and Organelles Resources at Addgene.org...Plasmids Find Plasmids for FRET Find Plasmids for Subcellular Localization ...

...down to one, key concept: Creating a culture of excellent, effective communication between all members of...meetings with team members (don’t become known for cancelling these); ask for feedback and input on multiple...

...Apparently, a good Jedi does not necessarily an excellent mentor make. Are your current mentors/advisors...What Kind of Mentor Do I Want To Be?” In this excellent essay Acclimatrix, an assistant professor at a...

...reading Toxic Academic Mentors by @drmellivora, an excellent blog post about workplace bullying featured on...the first place. The good news There are many excellent mentors out there. With some good pre-work you...

...would rather fund excellent people with mediocre ideas than mediocre people with excellent ideas. I am lucky...

...as an opportunity to practice communicating.  Excellent scientists learn from advisers and other mentors...help graduate students in all STEM fields become excellent science communicators. To empower graduate students...

...text for the example image. We’ll wait!  Done? Excellent! We asked our workshop participants to do the ...heard of alt text before, they all came up with excellent alt text for their practice image. We know; we...

...of Professor Richard Murray. Murray’s lab uses cellular extracts to perform transcription and translation...develop and test hypotheses. Students then use cellular extracts as a means to rapidly prototype DNA circuits...

...Professional Career Coaching can be an excellent tactic for scientists making a career shift or who wish...Consulting is part of that group and she is especially excellent in coaching for communication skills. Industry...

...of experience in [field of research], and have excellent [list the most important skills for the position... specific examples. Instead of saying “I have excellent communication skills” try giving an example, like...

... training is abundant, helpful, and sometimes excellent. Finally, as I said in the AMA, it’s incredibly...topics to the Addgene blog The blog has many other excellent posts on science career topics as well. Instead...

... in the genome. These technologies rely on the cellular DNA repair machinery, which can lead to high frequency... C (2000) Hugo De Vries: from the theory of intracellular pangenesis to the rediscovery of Mendel. Comptes...