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  1. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...The underlying method was developed in 1902 by Richard Zsigmondy and Henry Siedentopf to enhance the microscopic...using cameras with electron multiplying couple-charged device (EMCCD) or complementary metal-oxide semiconductor...

  2. Great Results Start with Great Standard Curves

    Type
    Blog Post
    Published
    Aug. 15, 2024, 1:15 p.m.
    ...final readout. When preparing a standard curve, change pipette tips and mix thoroughly by inversion or...dilution series, use consistent dilution factors, change pipette tips regularly, and mix samples very well...

  3. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    Published
    July 27, 2023, 1:15 p.m.
    ...genomic sequence from one another) — by various mechanisms, only one of the two strands’ DNA counterparts...γ-Retrovirus Guide References Cullen, B. R. (1992). Mechanism of action of regulatory proteins encoded by complex...

  4. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...Cancer Data Science team at Broad, they were able to change their analysis to use a corrected genome map. Now...): 250-252. Carlson J, Henn BM, Al-Hindi DR, Ramachandran S. Counter the weaponization of genetics research...

  5. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...and likely functions as a bacterial DNA repair mechanism. The pathway is ubiquitous, with successful recombination...placing homologous ends on DNA using polymerase chain reaction." Biotechniques 10.1 (1991): 62-66. PubMed...

  6. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...generation of the RNP complex.  While one option is to purchase the Cas9 protein and a gRNA from a commercial ...performed rapidly and, in many cases, with reduced chance for off-target effects. As the CRISPR field continues...

  9. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...occurring animal model, the RPE65−/− dog, was characterized and found to suffer from early and severe visual.... PubMed PMID: 29983335. 9. Day TP1, Byrne LC, Schaffer DV, Flannery JG, 2014. Advances in AAV vector ...

  10. DNA Purification Without a Kit

    Type
    Blog Post
    Published
    Feb. 11, 2020, 2:15 p.m.
    ...but the addition of a high concentration of a chaotropic salt (guanidine hydrochloride for the plasmid... glass milk, gel slices are first placed in a chaotropic salt sodium iodine (NaI) solution. NaI has two...

  11. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...journal club should aim to have many of the same characteristics as a successful meeting: a clear purpose and...success” of the journal club. This can be a challengingly vague metric but there are a few things you ...

  12. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    Published
    May 30, 2019, 12:44 p.m.
    ...identification of DNA binding proteins, this method can characterize nucleic acid-nucleic acid interactions at a ...2017). This upregulates expression of IGF2, a characteristic of many human malignancies. Additionally, the...

  13. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...college student searching couch cushions for spare change, desperate to extract a few more photons from a...Fluorescent Protein Fusions Request Plasmids from the Michael Davidson Collection Find Fluorescent Protein Kits...

  15. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...transformed with a micropulser using an electric charge to help the DNA enter the cells. Some samples require... to be read one at a time. 1D barcodes can be purchased from many companies such as Corning. Tubes can...

  16. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ... included a simple corn hole set and a cooler. Chances are one of your colleagues has a frisbee at least...where to be and when. This pre-planning will avoid chaos and delays in getting the fun started. I’m aware...

  17. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...lentiviral and retroviral packaging vectors are not interchangeable. While these differences may seem subtle they...the generation used to package the virus does not change the generation of the transfer vector. Q5: What...

  18. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ... been converted to an alanine (D10A or H840A), changing its double-strand cut to a single-stranded nick...Walton et al., 2020). Further, this group also characterized a second Cas9 variant, SpRY, which cuts at NRN...

  19. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...destination vectors prior to recombination, and it is exchanged with the gene of interest during the BP or LR ...Multiple Hosts. Curr Protoc Protein Sci. 2003 Feb;Chapter 5:Unit 5.17. PubMed PMID:18429245. 3. Ptashne, ...

  20. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...Although different types of cancer have different challenges and obstacles to overcome, mesothelioma sufferers...of immunotherapy is the use of drugs (or other mechanisms) that work through body’s immune system to fight...
Showing: 601 - 620 of 793 results