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...map these neuronal networks. For almost three decades, recombinant Adeno-associated virus (AAV) has been...symbol of opposition to the teaching of intelligent design in public schools and was started in 2005 in Kansas...

...deactivation method in zebrafish are short, modified oligonucleotides called morpholinos. When injected into zygotes...CRISPR/Cas9-mediated insertion of foreign DNA. Despite these caveats, several features of zebrafish—including...

... a receptor for PHP.B. Read on to learn how the design of PHP.B may have contributed to this mouse-specific...Cre-dependent selection yields AAV variants for widespread gene transfer to the adult brain." Nature biotechnology...

...updated Aug 20, 2020. Cas9 can be used to modify any desired genomic target provided that (1) the sequence is... may be unintentionally mutated along with the desired target locus. The PAM sequence is of particular...

...polymorphisms (SNPs) from human saliva. SHERLOCKv2 provides a method to detect nucleic acids with high sensitivity...per second. A quantifiable fluorescent signal designates the presence of your DNA of interest, in this...

...staining. Antigen-containing samples often include undesirable molecules that may bind non-specifically to assay...specific than other methods. In addition, the assay includes both primary and secondary incubation steps, increasing...

...replicate—but not always to the same extent. As described in our previous Origin of Replication post, DNA...replication. For example, the plasmid ColIb-P9 encodes the protein RepZ to drive its own replication. ...

...protocols, titering methods, and quality control are described below. AAV Production AAV distributed by Addgene...) are generated from lentiviral preparations (described above) that are subject to a concentration step...

..., and a PhD is not always necessary. The job description and the candidate requirements (and maybe also...without being too commercial or “sales-y”. The work includes reviewing customer data sets, troubleshooting ...

... Environmental Plasmids blog). Scientists have designed small, high copy incompatible plasmids that lead...Pubmed PMID: 25889267 Ebersbach G, Sherratt DJ, Gerdes K (2005) Partition-associated incompatibility caused...

...was that kid who used recycled notebook paper, despite the fact that it was gray. He has worked in molecular...molecular and microbiology for the last two decades as a staff scientist, but before going to graduate ...

...tumors.  This process is not quick. It can take decades from the first exposure to asbestos to the time...T-cells to more easily recognize and cause the destruction of cancer cells. Pembrolizumab has shown some...

...around $45,000, but as I’ve discussed elsewhere (slides here) there are many postdocs paid at much lower...expected for postdocs in many cases should be as described in the notice of the new NRSA levels and in the...

...best to start from scratch. Obtain a vial of the desired cell line from a reputable distributor such as ...extreme changes in osmotic pressure and pH can destabilize the viral envelope. One drawback of the multiple...

... live cells in real time. Previously, we have described a number of practical considerations that should... herpesvirus.       References 1. Snapp, Erik. "Design and use of fluorescent fusion proteins in cell ...

...flattening the curve, it has become evident that widespread testing is crucial in controlling this pandemic...one temperature. Two Cas12a-crRNA complexes are designed, each binding to one strand of the reverse-transcribed...

...antibody-based technique, validation and proper experimental design are essential. In this article, we’ll go through...steps include: counterstaining, mounting sample on slides, and confocal   Figure 2: High-level overview...

... A) Schematic of workflow. B) Detail of spacer peptides (3HB: 11-nm 3-helix bundle; SAH: 60-nm single ...cryo-EM samples from damage at the AWI. Plus, tardigrades!  Find RvLEAMshort here!    Abe, K. M., & Lim...

...performed best when paired with the V106W mutation (described earlier) to minimize off-target editing. Base ...final base editor that was administered to KJ was designated NGC-ABE8e-V106W. The name is hardly flashy, but...

... – including undermining personal integrity, destructive innuendo and sarcasm, persistent teasing, name...impossible deadlines, unnecessary disruptions Destabilization – including failure to acknowledge good work...