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Showing: 601 - 620 of 766 results

  1. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    Published
    Oct. 5, 2021, 1:15 p.m.
    ...the probe for their needs. You’ll find basic information on NIR FPs, their properties and applications...mIFP6. Learn more about the characteristics, performance, and applications of near-infrared calcium indicators...

  2. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    .... I will emphatically deny that I have a magic formula to guarantee a reader the faculty job of his/her... I would not recommend putting these into slide format. You want people to focus on you and your ideas...

  3. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...found that many researchers feel that they lack formal training in a variety of transferable skills. At..., the task will likely be much easier. Having a formal plan for your day will make this easier - if you...

  4. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    Published
    June 27, 2024, 1:15 p.m.
    ...day. Note that intracellular labeling must be performed only after viability staining and surface labeling...surface labeling. Intracellular labeling can be performed on cytosolic and/or nuclear markers. Lastly, stains...

  5. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ... use, but when you need to juggle filming and performing on camera, it’s a lot to handle. We decided the...talent could just focus on their interview or performance. By this point, towards the end of the summer...

  7. Exploring Applications of the Bioluminescent HiBiT Tag

    Type
    Blog Post
    Published
    Oct. 2, 2025, 1:15 p.m.
    ...also known as KDR), orchestrating blood-vessel formation. Precise, real-time pharmacology of this target...modifications to the binding sites and various isoforms led to subtle changes in co-receptor dynamics,...

  8. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ... iGluSnFR3 mutants, identifying the two  best-performing variants as iGluSnFR4f (fast deactivation) and...11%. It has higher efficiency and lower indel formation than methods relying on homology-directed repair...

  9. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    Published
    Jan. 16, 2025, 2:15 p.m.
    ...of one AAV serotype with the capsid of another. Formally, pseudotypes are described with two numbers separated...using the genomic structure of AAV2, so they are formally called rAAV2/1, rAAV2/2, and so on, although we...

  10. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...submerged in a tank filled with a transfer buffer formulated to conduct the electric current. The wet transfer...addition, the thickness of the band provides information about the relative abundance of the protein in...

  11. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations in animal models or cells...piggyBac™ for gene editing allows you to efficiently perform seamless genetic manipulations, while avoiding ...

  12. When Fidelity Matters: A frank discussion about ligase fidelity

    Type
    Blog Post
    Published
    March 6, 2018, 1:49 p.m.
    ... studies through a high-sensitivity multiplexed format (21, 27). This methodology has been used to rapidly...to screen additional ligases, conditions, and formulations and has led to the development of the new HiFi...

  13. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...scholar/student: visiting from another university to perform research   Why should I try research? Personally...reflect on what I wanted for my own future. Informational interviews with people who have taken career...

  14. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ...and Agrobacterium-mediated transformation are available. For more information on this system, check out...

  15. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...certain circumstances) Current base editors can perform any base-to-base conversion in principle, but some...the process of double nicking increases indel formation slightly. Designing the sgRNA with a spacer that...

  16. Addgene Launches Partnership with IPI

    Type
    Blog Post
    Published
    June 13, 2023, 1:15 p.m.
    ...Antibodies 101 blog series.  Stay tuned for more information on our blog and social media! ...

  17. Viral Vectors 101 eBook 2nd Edition

    Type
    Blog Post
    Published
    July 11, 2024, 1:15 p.m.
    ... of vectors, capsids, and tools; and helpful information on everything from producing your own vectors...

  20. It’s CRISPR Week at Addgene!

    Type
    Blog Post
    Published
    May 13, 2019, 12:01 p.m.
    ...editing to pooled library screening. It also has information for planning your CRISPR experiment. For a quick...
Showing: 601 - 620 of 766 results