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...resources and databases are scrutinized, and this information is enriched with your technical knowledge and...data sets, troubleshooting protocols, analyzing information, and solving customer problems. Technical support...

...making it easier for comparable studies to be performed on a cell line even decades after it is initially...passage the cells (as well as recording this information in your lab book and on the actual flasks of ...

...faciliate proper folding; may increase multimer formation Δ(ara-leu)7697 ΔlacX74 ΔphoA PvuII phoR araD139... T7 RNAP expression alleviates inclusion body formation. Expression vector cannot have p15A origin of ...

...day. Note that intracellular labeling must be performed only after viability staining and surface labeling...surface labeling. Intracellular labeling can be performed on cytosolic and/or nuclear markers. Lastly, stains...

.... I will emphatically deny that I have a magic formula to guarantee a reader the faculty job of his/her... I would not recommend putting these into slide format. You want people to focus on you and your ideas...

...the probe for their needs. You’ll find basic information on NIR FPs, their properties and applications...mIFP6. Learn more about the characteristics, performance, and applications of near-infrared calcium indicators...

... use, but when you need to juggle filming and performing on camera, it’s a lot to handle. We decided the...talent could just focus on their interview or performance. By this point, towards the end of the summer...

...found that many researchers feel that they lack formal training in a variety of transferable skills. At..., the task will likely be much easier. Having a formal plan for your day will make this easier - if you...

...hour or so, the sample is immediately ready to transform into competent cells. The master mix of enzymes...Gibson assembly master mix for 1 hour, and then transform into competent cells. The success rate of this...

...also known as KDR), orchestrating blood-vessel formation. Precise, real-time pharmacology of this target...modifications to the binding sites and various isoforms led to subtle changes in co-receptor dynamics,...

... iGluSnFR3 mutants, identifying the two  best-performing variants as iGluSnFR4f (fast deactivation) and...11%. It has higher efficiency and lower indel formation than methods relying on homology-directed repair...

...of one AAV serotype with the capsid of another. Formally, pseudotypes are described with two numbers separated...using the genomic structure of AAV2, so they are formally called rAAV2/1, rAAV2/2, and so on, although we...

...submerged in a tank filled with a transfer buffer formulated to conduct the electric current. The wet transfer...addition, the thickness of the band provides information about the relative abundance of the protein in...

...can be combined with CRISPR/Cas9 to efficiently perform genetic manipulations in animal models or cells...piggyBac™ for gene editing allows you to efficiently perform seamless genetic manipulations, while avoiding ...

...scholar/student: visiting from another university to perform research   Why should I try research? Personally...reflect on what I wanted for my own future. Informational interviews with people who have taken career...

... studies through a high-sensitivity multiplexed format (21, 27). This methodology has been used to rapidly...to screen additional ligases, conditions, and formulations and has led to the development of the new HiFi...

...and Agrobacterium-mediated transformation are available. For more information on this system, check out...

...certain circumstances) Current base editors can perform any base-to-base conversion in principle, but some...the process of double nicking increases indel formation slightly. Designing the sgRNA with a spacer that...

...Antibodies 101 blog series.  Stay tuned for more information on our blog and social media! ...

... of vectors, capsids, and tools; and helpful information on everything from producing your own vectors...