We narrowed to 816 results for: lars;
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TypeBlog PostPublishedApril 22, 2021, 1:15 p.m.... Some of our lentiviral plasmids include popular envelope and packaging plasmids as well as transfer... great fit for any scientist, whether you use molecular biology tools in your research or not! And learn...
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Inntags: Innovative Protein Epitope Tagging
TypeBlog PostPublishedNov. 10, 2015, 3:30 p.m.... that 20% of tagged proteins did not localize similarly to their untagged counterparts. These findings... for examining protein-protein interactions, particularly in single-molecule or high-throughput applications... -
What's New in CRISPR - Spring 2019
TypeBlog PostPublishedMarch 21, 2019, 12:47 p.m....Cre-recombination. Read the paper in Frontiers in Molecular Neuroscience Find the AAV-based SaCas9 genome... deposited “Mobile-CRISPRi” plasmids, a set of modular CRISPRi tools that can be used in diverse bacteria... -
Science Communication Snapshot: DayCon 2015
TypeBlog PostPublishedJune 17, 2015, 9:04 p.m....researcher. The future of DayCon DayCon and other similar events show how meaningful science outreach can...event and to expand the conference to accommodate a larger number of attendees. This first-year event’s success... -
Quick, Versatile Plant Transgenesis with GreenGate Plasmids
TypeBlog PostPublishedJan. 23, 2014, 3:23 p.m.... possible to quickly and efficiently assemble a large number of building blocks with just two or three...system based on the daily needs of an advanced molecular plant science lab.” Introducing GreenGate As ... -
Editor's Choice, August 2016
TypeBlog PostPublishedSept. 2, 2016, 1:32 p.m....of NgAgo In particular, we need to thank Pooran Dewari who wrote this month’s most popular blog post on... -
Modulate the Activity of 17 Signaling Pathways with One Kit!
TypeBlog PostPublishedMarch 22, 2016, 2:30 p.m.... of time, cancers become resistant to drugs, particularly targeted therapies,” said Kris Wood of Duke ...drug resistance Wood wanted to find out which particular pathways drive resistance to specific drugs in... -
Scientist Networking: What is an Informational Interview?
TypeBlog PostPublishedJuly 1, 2014, 3:42 p.m.... meeting with someone to ask about a job or a particular position at their company, do NOT call this an... is a good time to ask personal questions about salary, opportunities for advancement, challenges and ... -
Top 10 Open Science Developments of 2013
TypeBlog PostPublishedDec. 19, 2013, 3:46 p.m....in open science this year. Here it goes, in no particular order, my top 10 open science developments, 2013...-respected and high-profile universities. We particularly recommend Eric Lander's intro to biology course... -
SciComm with the Experts at Science in the News Part 2
TypeBlog PostPublishedApril 27, 2017, 2:30 p.m....programs are discussed below). Chpater 3: SITN Popularity and Events - Amy discusses some of the programs...programs SITN runs and describes some of the more popular topics from the Science by the Pint series. Chapter... -
Seeing Red: Simple GFP Photoconversion
TypeBlog PostPublishedAug. 4, 2015, 2:30 p.m.... are you’ve used a GFP derivative to conduct subcellular localization studies or make a reporter construct...this technique may not be appropriate for high-pH cellular compartments. Sattarzadeh et al. have shown that... -
Three Key Considerations for Precise Neuronal Targeting Using AAV Technologies
TypeBlog PostPublishedNov. 27, 2019, 2:03 p.m....strategies for using combinations of AAV-based molecular tools for targeted neuronal manipulation. The ...of interest: Cell-type specific expression of molecular tools and sensors Combinations of classical genetic... -
Increasing Your Work's Visibility with Addgene: Citation, Search, and Collections
TypeBlog PostPublishedMarch 10, 2016, 3:30 p.m....a network of researchers who may be working on similar projects and should make it easier for them to ...missing an important collection or if you think a particular plasmid or type of plasmid is missing from our... -
Mapping the 4D nucleome with CRISPR/Cas9
TypeBlog PostPublishedAug. 11, 2015, 2:30 p.m....facilitating the live study of chromatin dynamics at a particular locus. A limitation of this system is the single-color...studies of cell cycle progression, epigenetics and cellular reactions to external stimuli. It could also have... -
Cre-ating New Methods for Site-specific Recombination in Drosophila
TypeBlog PostPublishedMay 12, 2015, 1:32 p.m....Cre-lox recombination is an incredibly useful molecular biology tool, but like any biological system, ... yeast recombinases: KD (from Kluyveromyces drosophilarum), R (from Zygosaccharomyces rouxii), B2 (from... -
London Calling - Addgene’s New Europe Office in London
TypeBlog PostPublishedJune 10, 2014, 3:20 p.m....dimerization of HLA B27, involved in the onset of spondylarthritis, using Bioluminescence Resonance Energy Transfer...the University of California, specialising in Molecular Biology. She has worked in a range of labs - cancer... -
In Living Color: The Skinny on In Vivo Imaging Tools
TypeBlog PostPublishedMarch 27, 2014, 5:14 p.m.... nm will pass through animal and human tissues largely unimpeded. The challenge then was to develop fluorescent... Ultrasound wavelengths, on the other hand, are larger than the size of a cell, so they scatter much less... -
CRISPR 101: Cas9 Nickase Design and Homology Directed Repair
TypeBlog PostPublishedMarch 15, 2018, 12:59 p.m.... small deletions, while H840A is biased towards large insertions. These profiles are likely due to the... Oligonucleotides) Use 100 nt homology arms for large insertions (long ssDNA; IDT Megamer® ssDNA Fragments... -
Hot Plasmids and Viral Preps - July 2021
TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....internal control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection... -
Oh, The Places You Can Go: Careers in Science Communication - Science Writing
TypeBlog PostPublishedOct. 3, 2017, 12:11 p.m....writer When Hans was finishing up his PhD in Molecular Neuroscience at the University of Iowa, he was...over hosting them. Since then, webinars make up a larger part of Hans’ workload. “I am currently getting...