We narrowed to 714 results for: met

1. Build Your CRISPR Vocabulary

   Type

   Blog Post

   Published

   July 2, 2024, 1:15 p.m.

   ...system for laboratory applications. At Addgene, we sometimes refer to the programmable guide sequence as the...

2. Year of the Camelid: Antibody Style

   Type

   Blog Post

   Published

   May 9, 2024, 1:15 p.m.

   ... Genetically modified mice offer an alternative method to producing single-domain antibodies without the...

3. Five Questions to Ask If You're Deciding Between a Master's or PhD Program

   Type

   Blog Post

   Published

   Feb. 16, 2021, 2:15 p.m.

   ...job descriptions sound interesting? Is the work something you can see yourself enjoying?  To find master...

4. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

   Type

   Blog Post

   Published

   March 15, 2018, 12:59 p.m.

   ...post were adapted from an IDT webinar: Optimized methods to use Cas9 nickases in genome editing. For more...

5. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

   Type

   Blog Post

   Published

   Nov. 1, 2017, 2:04 p.m.

   ...proposals, a few PhD theses, and occasionally posting something science-related on Facebook. “I think this helped...

6. Addgene at the ASM Microbe 2016 Conference

   Type

   Blog Post

   Published

   July 7, 2016, 2:30 p.m.

   ...Addgene's Microbiology Pages Read Our Post on CRISPR Methods for Bacterial Genome Engineering Learn how to Network...

7. Management for Scientists: Managing vs. Leading

   Type

   Blog Post

   Published

   March 31, 2015, 4:08 p.m.

   ...collaborating effectively with other teams. While they sometimes dig in and do some of the work, they must be more...

8. Management for Scientists: Giving Feedback

   Type

   Blog Post

   Published

   Feb. 6, 2015, 2:46 p.m.

   ...person - intent is usually good. Don’t pretend something went well when it didn’t… but don’t “punish” someone...

9. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

   Type

   Blog Post

   Published

   Jan. 15, 2015, 1:50 p.m.

   ...modifying, and improving the CRISPR-Cas9 system. Mehmet Fatih Bolukbasi from Scot Wolfe's lab described...

10. Choosing Your Perfect Empty Backbone

    Type

    Blog Post

    Published

    Aug. 19, 2014, 3:39 p.m.

    ...in fly culture cells like S2 and carry the metallothionein promoter (e.g. pMT-puro). Worm vectors There...

11. Plasmids 101: Origin of Replication

    Type

    Blog Post

    Published

    Nov. 11, 2020, 2:23 p.m.

    ...started to die off. Have any questions? Need something clarified? If so please, post in the comments....

12. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type

    Blog Post

    Published

    Sept. 17, 2020, 1:15 p.m.

    ...like we’re being buffeted by wind and waves and sometimes wonder if we can take another blow. Back in March...

13. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    Published

    Oct. 10, 2019, 12:41 p.m.

    ...frameworks and structure-based framework engineering. Methods, 34(2), 184–199. PubMed PMID: 15312672. Inbar, ...

14. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type

    Blog Post

    Published

    April 25, 2019, 3:01 p.m.

    ...derived from Branchiostoma lanceolatum." Nature methods 10.5 (2013): 407. PubMed PMID: 23524392. PubMed...

15. New and Upcoming Viral Vectors - Spring 2019

    Type

    Blog Post

    Published

    March 26, 2019, 1:40 p.m.

    ...inhibitory neurotransmitter in vertebrates, but methods for directly imaging GABA have been limited. Like...

16. Summer SciComm Series: Master of SciComm

    Type

    Blog Post

    Published

    July 21, 2022, 1:15 p.m.

    .... Often, these companies believe they possess something that will truly benefit others. Herein, a good...

17. Addgene Begins Distribution of Recombinant Antibodies

    Type

    Blog Post

    Published

    March 28, 2022, 4:30 p.m.

    ...through Rigor and Transparency of Experimental Methods. Neuron 90(3):434-6. https://doi.org/10.1016/j....

18. Three Tips for Preventing Viral Plasmid Recombination in Your Samples

    Type

    Blog Post

    Published

    Sept. 14, 2021, 1:15 p.m.

    ..., Tolmachov OE, Zambetti LP, Tchetchelnitski V, Mehmet H (2012) Remarkable stability of an instability-prone...

19. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type

    Blog Post

    Published

    Sept. 21, 2023, 1:15 p.m.

    ...0022155413511620  Lu QL, Partridge TA (1998). A New Blocking Method for Application of Murine Monoclonal Antibody to...

20. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type

    Blog Post

    Published

    Aug. 31, 2023, 1 p.m.

    ...fact: A 1 cm block of polymer can generate many meters of fibers!     Fig. 1 - Overview of the...