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We narrowed to 794 results for: mpl

Showing: 601 - 620 of 794 results
  1. Addgene Begins Distribution of Recombinant Antibodies

    Type
    Blog Post
    Published
    March 28, 2022, 4:30 p.m.
    ...educational materials to our repository of open samples. We are, and have always been, a community resource....43322 Bandrowski, AE, Martone, ME (2016) RRIDs: A Simple Step toward Improving Reproducibility through Rigor...
  2. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type
    Blog Post
    Published
    June 9, 2016, 2:30 p.m.
    ...able to mediate homology-directed repair when a template is supplied. Find Plasmids from the NgAgo Publication...(so called “gDNAs”) rather than the RNA guides employed by Cas9 or Cpf1. These short, 24 base DNA guides...
  3. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    Published
    Jan. 21, 2014, 3:08 p.m.
    ..., in turn, the big picture of the project. For example, how can future regulations impact your work? What...sounds interesting, TAKE ACTION! Taking action: Examples of extracurricular activities The table below...
  4. Trading Spaces - Visiting Scientist Edition

    Type
    Blog Post
    Published
    Aug. 22, 2023, 1 p.m.
    ...from a month to a year. The researcher remains employed by their home institute, meaning the host university...and a priority list of experiments you hope to complete. Share this plan with your host prior to arrival...
  5. Hot Plasmids - January 2023

    Type
    Blog Post
    Published
    Jan. 31, 2023, 2:15 p.m.
    ...JA. Precise transcript targeting by CRISPR-Csm complexes. Nat Biotechnol. 2023 Jan 23. doi: 10.1038/s41587...antibodies allows you to use them in parallel to help amplify your signal - giving you the benefit of a polyclonal...
  6. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ... binding affinity to major histocompatibility complexes (MHC class I and II).  There are multiple algorithms...approach The in silico approach cannot replicate the complexity of antigen processing events that take place ...
  7. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ... proteins should be separated by 50-60 nm. For example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm...protein of interest has a similar spectrum. For example, here's a screenshot from the Chroma Spectra Viewer...
  8. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    Published
    June 6, 2023, 1:15 p.m.
    ...that use reverse transcriptase to synthesize complementary DNA copies of their genome upon infecting a ... These viruses have a cargo capacity of ~ 8 kb, ample room for most experimental needs, and can transduce...
  9. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction...targeting an exon in your gene of interest, for example. Some customization is required, but many viral...
  10. Why and how to start studying chromatin (with Addgene's help)

    Type
    Blog Post
    Published
    June 12, 2018, 11:40 a.m.
    ...interact with each other. Chromatin regulation is complex and influences--directly or indirectly--many other...phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling...
  11. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade... next to a different constant domain gene. For example if a B cell switches to producing IgE antibodies...
  12. Communicating Your Science With Help From ComSciCon

    Type
    Blog Post
    Published
    July 19, 2018, 1:12 p.m.
    ...an interview setting, this means persuading an employer that your past work has prepared you to contribute...audience and ask for their feedback before you complete your work. If you’re giving a public talk, ask...
  13. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...conditions in order to maximize the mutation rate. For example the Phage Assisted Continuous Evolution (PACE) ...interest. A gRNA is used to direct the PolI3M-nCas9 complex to a DNA site of interest, which nCas9 nicks and...
  14. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ... look at how we create this content, using the example of our Antibody Guide, which was published alongside...require updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few ...
Showing: 601 - 620 of 794 results