We narrowed to 904 results for: CAN

...reverse transcriptase so they can transpose. Both class I and II transposons can be autonomous or non-autonomous...transposon lands. For example, in bacteria, transposons can move antibiotic resistance genes between plasmids...result in human disease. While transposon insertions can be detrimental, it also drives genomic evolution ... (RT) enzyme to convert back into DNA. Then they can be inserted into new target sites. Created in BioRender.com...BioRender.com.   Autonomous vs. non-autonomous TEs You can think of autonomous transposons as “complete transposons... is provided in trans so that a gene of interest can be inserted between the transposon’s LTRs, similar... DNA footprint sequence after excision. piggyBac can mobilize DNA over 100 kB in size and is active in...

...responses (Gunaydin et al., 2010). This can affect how rapidly you can activate or inhibit a cell in succession...only light. Optogenetics, or light activated tools, can control everything from electrical activity to gene...they activate downstream signaling pathways that can change both the membrane potential and a host of ...intracellular proteins come into play. Photoswitches can be used to activate or suppress secondary signaling...pharmacology techniques allow. Multiple photoswitches can be used in a single pathway to activate various protein...vectors have an optimal wavelength to use but can be activated by a range of wavelengths. While this may...flexibility when activating the optogenetic tool, it can also cause problems when using multiple optogenetics...

...off-target sites that they can later sequence for mutations. One method a researcher can use to test predicted...and assayed phenotype. Verifying these connections can help alleviate the reproducibility crisis in biology... DNA. When these strands hybridize, the nuclease can detect strands with mismatches and cleave them. The.... These techniques differ in important ways, but can also complement one another by providing both broad...genome sequencing. Used in concert, these approaches can provide the researcher with a reasonable level of...2015). Low detection rates mean the investigator can be relatively certain that their samples don’t have...2015) for cell-based experiments. These strategies can be used in concert with in silico prediction to create...

... their bulk (~27 kDa) can perturb folding or trafficking and their detection can suffer from photobleaching...These challenges can include background autofluorescence from the plates or media, which can obscure weak ...biology. Fluorescent fusions utilizing bulky tags can disrupt normal cell biology and make it hard to capture...excitation generating reactive oxygen species that can lead to phototoxicity, perturb biological processes...trafficking (Hall et al., 2012). Antibody-based tools can also be combined with the HiBiT tag to provide complementary...HiBiT-tagged targets in translational research Researchers can incorporate HiBiT with a wide range of workflows,...Cracking the code of 'undruggable' targets Researchers can design drugs to bind to well-defined ligand-binding...

...GAL80 repressor can bind to GAL4, partially inhibiting the binding of GAL4 to UAS. One can therefore place...previous post, we discussed inducible promoters, which can be switched from a default OFF to an ON state, and...Today, we’ll look at repressible promoters, which can be switched from a default ON to an OFF state, as... Like inducible promoters, repressible promoters can be regulated via positive or negative control. Positive...binds the repressor protein, the repressor protein can bind to the operator. The bound repressor then prevents...native context, the tetracycline repressor (TetR) can bind to the tetracycline operator sequences (TetO...in mammalian cells, and TRE-containing promoters can be used in the repressible manner described above...

...table below and can be requested through Addgene. Their plasmid maps and GenBank files can be found on their...on the state cannot be undone and also limit the set of possible downstream states that can be achieved...leveraging qPCR data from multiple primer pairs, one can infer the predominant state of registers within a...register. However, each Sanger sequencing reaction can only be performed on one register at a time. If a...be considered as an alternative. The qPCR method can be performed on a sample of multiple registers. However...based on transcriptional regulation, as long as they can control recombinase activity. For example, post-transcriptional...software that automates the design of registers that can be used to execute user-specified two-input gene ...

..., these challenges can be considerably reduced. The piggyBac™ transposon system can be combined with CRISPR... known upper limit [1]. The piggyBac™ technology can be used for numerous applications, including transgenesis...with piggyBac™, the number of integration copies can be tightly controlled by titrating the transposase... of integrations per cell and a super-piggyBacTM can be used to get an even greater number of integrations...interest. Importantly, by varying these ratios, you can either maximize or minimize expression levels of ...dominant-negative mutant. Reducing the copy number can also mitigate the deleterious effects of expression...overcome this obstacle, a positive selection cassette can be incorporated, and used during the differentiation...

...lindy hop swing dance event,” Zheng says (networking can happen anywhere!). The pair quickly teamed up to ... phage matches. Email [email protected] if you can help! — Dr Steffanie Strathdee, 🗡️Superbug Slayer...right phage for the job. Phages are viruses that can infect and then kill bacteria they enter. But to ...have been exhausted. Theoretically, phage alerts can be sent out to the directory earlier, but the researchers...phage therapy, acceptable endotoxin levels, etc. can vary depending where treatment occurs. In addition...Sacher hopes that as Phage Directory expands, it can also fill this role by cataloging and archiving information...

... Protein purification can be one of the most stressful lab activities. Working with proteins requires...the best way to express one’s protein of interest can save a lot of time later. Protein purification specialists...protein - two parameters that affect how well E. coli can produce your protein. Structural information for ...and PDBsum. After acquiring this information, you can then pick an expression vector and expression system...properly. If a TEV protease site is present, TEV can remove these tags from the purified protein. For ...the use of a thioredoxin A (TRX)-containing vector can improve disulfide bond formation. pCri also includes...

... microscope, you too can become an artist. Scientists moonlighting as artists can compete in the Nikon...modern biologists focus on one aspect alone. They can study molecules, analyze terabytes of information... of the creature they deal with.” So photography can be used as a way to better understand and appreciate...biological importance. More of Daniel's stunning work can be found on his website http://www.microworldsphotography.com...holding scientific art competitions where creations can go on display for all to enjoy. The largest competition...