We narrowed to 803 results for: Cre

... to its final destination. As an example, most secreted proteins are produced in the endoplasmid reticulum...golgi and then transported in vesicles via the secretory pathway to their subcellular or extracellular ... they are finally degraded (2, 3). Once you've created your fusion protein, it's important to validate... to make any fluorescent protein constructs you create in your own work available to the Addgene community...Edition. 3.  CureFFI.org. Cell Biology 04: The Secretory Pathway. 4. Dunn, Kenneth W., Malgorzata M. Kamocka...

... aaRSs in these processes. To create the antibody toolbox, they screened antibody candidates using a synthetic...fourteen aminoacyl tRNA synthetases (aaRS) have been created, characterized, and validated by the Gräslund lab...cross-reactivity, antibody candidates underwent affinity screening to select for slow off-rate binders. The best ...pathways By Gabrielle Clouse Claudia Vickers’ lab has created a novel nanocompartment for transporting proteins...

... deletions or homology-directed repair (HDR) to create precise genome edits. But have you heard of another...depositor Takashi Yamamoto’s lab has harnessed MMEJ to create a new method for CRISPR gene knock-in, termed PITCh...PITCh plasmids, GFP knock-in cell lines can be created in about a month and a half, without the need for...PITCh-gRNA, and the locus-specifc gRNA. The PITCh-gRNA creates two DSBs in the PITCh plasmid to release the cassette... alter expression of the gene it follows. For increased versatility, it would be advantageous to adapt...

...Website: http://protocols.io/ Protocols.io is the creation of ZappyLabs. Currently in beta testing, the software...The site allows you to comment on protocols and create lists of your favorite protocols for future reference...of the early protocol sharing websites. It was created in 1999 and allows scientists to contribute protocols...web-based lab management tool that allows labs to create accounts in which their members can share protcols...your lab’s needs. At Addgene, we’ll continue to create new molecular biology educational resources, such...

... or the creation of bicistronic or multicistronic vectors. Unlike promoters which will create unique mRNA...resistance gene. These reporters allow you to easily screen or select for cells that are expressing your gene...interest at high levels. Unlike vectors that express screenable or selectable markers from a unique promoter,...now download Addgene's new eBook! Our goal was to create a one-stop reference guide for plasmids. We've ...

...researchers saw an 11-fold increase in mRNA levels after 2 hr and a 400-fold increase after 30 hr. The system...(CIB1) proteins. The goal of both groups was to create a system that would use light to turn on and off...domain to each of the Cas9 fragments, the authors created a photoactive Cas9 tool, as shown in the figure...binding and cleavage. This technique was applied to create photoswitchable spCas9, dCas9, and S. aureus Cas9...Gilgenast TG, Gong W, Beagan JA, Raj A, Phillips-Cremins JE (2019) LADL: light-activated dynamic looping...

... 2021). In fact, completion of Provost’s CURE increased students’ confidence in their research capabilities...projects have also been proposed as a mechanism for increasing diversity and inclusion in STEM disciplines (... Brownell, 2014; Duboue et al., 2022), as they increase student interest in scientific career paths while... Provost believes these networking experiences increase student enthusiasm, and research backs up that...undergraduate research experiences are a “CURE” that increases engagement by students and teachers. FEMS Microbiology...

...the templated DNA. Check out our blog post on increasing HDR efficiency to read more about optimizing ...pathways have been shown to be efficacious for increasing knock-in events (Yang et al., Int J Mol Sci)....culture media prior to and after Cas9 introduction increases HDR, just make sure to remove the inhibitors within...Early HR factors fused to Cas9 have been shown to increase HDR frequency by committing a break to performing...reading up on validating genome edits to find screening strategies for your experiment. Also learn about...

...large number of constructs for high-throughput screening of PPIs. For example, Bischof et al. (2018) generated...generated an open reading frame (ORF) library to screen PPIs among 450 transcription factors in live fruit...organisms lags behind. BiFC can also be used to screen for small-molecule inhibitors of a particular PPI...Here, one of the endogenous PIPs is expressed at increasing levels alongside your BiFC constructs. If your...co-expressing one of the endogenous PIPs should decrease the BiFC signal in a dose-dependent manner. This...

...CRISPR, and the challenges of science becoming increasingly politicized. It would be nearly impossible for...all of these things contributed to the steadily increasing distrust the general public has in science and...self-promotion, and personality is playing an increasingly important role in science. The science produced...reserved Franklins and Prashers will find it increasingly difficult to compete (for funding, etc.) with...chapter on deep learning addresses the dilemma of creating a technology that could eventually replace scientists...