We narrowed to 932 results for: Gene

...Resources on Addgene.org Visit Our Microbiology Pages Use the FX Cloning Sytem to Clone Your Gene of Interest...making it difficult to quickly identify possible gene function. In this post, I will describe a useful...done, this technique can also help pinpoint the genes within a sequenced genome that encode for functional...digesting phage genomic DNA into 1-3 kb (approximately gene-sized) fragments so that these fragments can be ...results in optimal likelihood of capturing a whole RBP gene without causing issues associated with cloning large...back to the original plate to identify which phage gene encodes that protein! First, block your membranes...for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages The problem: Host binding proteins...

... your antibody is validated! Genetic Strategies This approach uses a gene knock-down or knock-out approach...all sample types are amenable to genetic manipulation, nor can all genes be easily knocked down or out. ...bioinformatician) to look at RNA expression of your gene of interest across a set of human tissues.  From...Conveniently, you found a cell line in which the gene for your protein has been mutated using CRISPR/Cas9...plasmid encoding your protein of interest, known as Gene of Interest (GOI), and transfect it into your favorite...this protein, which we will call Negative Control Gene (NCG) (A). Then you lyse the cells and run a Western... post!  Resources on Addgene.org Find plasmids encoding antibodies at Addgene Stay tuned for ready-to-use...

... is easy. Ordering genes on plasmids online is a little trickier -- but at Addgene, we try to make the...here at Addgene. If you’ve called our customer service line or sent an email to help@addgene.org, you’ve...seller. However, Addgene is a bit different from other vendors. As a nonprofit, Addgene does not own the... bigger question: How does a customer place an Addgene order using a PO number? Why do I have to order...distribute it on behalf of depositing institutions. Addgene cannot fulfill orders based on PO documents that...information that is entered at checkout is used to generate the legal agreement (MTA), which streamlines the... with the recipient organization can create an Addgene account and place an order: a purchasing agent,...

...Welcome to Addgene, Aavery!       Original post: In December, we introduced you to Blugene’s new virus...March 6, 2020. After months of input and polling, Blugene's new virus friend finally has a name! We thank ...we’re excited to see your thoughts. Our mascot Blugene can’t wait either. Acknowledgements We would like...

...on the Addgene blog Learn more about Blugene's make-over Get a how-to guide on the new Addgene search... this form or the hashtag #BlugeneVirusFriend on Twitter. As with Blugene’s name, we’d like to give their...receive some newly designed Addgene swag. Have fun! Suggest a name for Blugene's new virus friend here Additional...eight years now has a new friend to join them. Blugene would like you to meet...  Hmmm, they need a name... we need your help in finding a good name for Blugene’s virus friend.  During the month of January,...

...knockout screening, sgRNAs guide Cas9 to cut targeted genes, creating mutations that prevent their expression...expression. The knockout cells can be used to identify genes that underlie a particular phenotype. Figure...knockout screens: sgRNAs to guide Cas9 to targeted genes. In these applications, however, nuclease-dead Cas9...contain thousands or millions of different books. Addgene’s pooled libraries are a little different: each ...are currently 390 pooled libraries available at Addgene, with more added to the collection all the time...barcodes help librarians track individual books, genetic barcodes allow researchers to identify specific...technologies multiply, so do CRISPR pooled libraries! Addgene’s collection also includes a base editing sensor...

...Subscribe to the Addgene newsletter for updates on all things Addgene; or subscribe to the Addgene blog for updates...request an AAV composed of an AAV plasmid in the Addgene repository and their choice of five different serotypes...comes to ordering high-quality materials from the Addgene repository. Read on to learn more.  What will be... be available?  Over 3000 AAV plasmids in the Addgene repository will be available for Packaged on Request...request. Request details can be accessed within your Addgene account. Preps will be available for request in...Once submitted, the request will be reviewed by Addgene for legal approvals and technical compatibility...Cart” button will appear with the request in your Addgene account.  Orders do not need to be placed immediately...

...throughput studies of optogenetics.  Common opsins used in zebrafish Optogenetics functions through opsins....  Additional resources on the Addgene blog Find all our optogenetics blog posts Read blog posts about... viral vectors Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral...Optogenetics, the use of light sensitive proteins (opsins) to manipulate cell activity, enables researchers...escape reflex. These traits, coupled with short generation times and high fecundity, make zebrafish ideal...are light sensitive proteins. Opsins used in optogenetic studies are not native to vertebrates and are...swimming  Researchers at the Isacoff lab applied optogenetics in zebrafish larvae to identify new neurons ...

...content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources on the Addgene blog Learn...about Addgene's Lab Tips video series Resources on Addgene.org Find lab protocols on the Addgene website...Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps... purity of all AAV viral lots. Aligning with Addgene’s mission to “accelerate research and discovery ...improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient...calculations on how to prepare a plasmid stock used to generate a standard curve, visit the AAV Titration by qPCR...website Watch Addgene's video collection ...

... time or location can be critical when, say, the gene is essential during development and a knockout is...the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics Guide... The Chow Lab has also generated a variety of membrane-recruitment optogenetic tools for manipulating ...a full toolbox and workflow for generating new Rho GTPase optogenetic systems (Berlew et al., 2022). Stopping...LOV, CRY2-CIB, and other optogenetic modules? Or want to find more optogenetic tools in our collection?...collection? Check out our Optogenetics Guide or browse our collections of optogenetics plasmids – and keep an eye...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be ...