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We narrowed to 928 results for: Gene

Showing: 311 - 320 of 928 results

  1. Technique: Probe Phage Genomes for Host Binding Proteins

    Type
    Blog Post
    Published
    Sept. 29, 2016, 2:30 p.m.
    ...Resources on Addgene.org Visit Our Microbiology Pages Use the FX Cloning Sytem to Clone Your Gene of Interest...making it difficult to quickly identify possible gene function. In this post, I will describe a useful...done, this technique can also help pinpoint the genes within a sequenced genome that encode for functional...digesting phage genomic DNA into 1-3 kb (approximately gene-sized) fragments so that these fragments can be ...results in optimal likelihood of capturing a whole RBP gene without causing issues associated with cloning large...back to the original plate to identify which phage gene encodes that protein! First, block your membranes...for a review, see Simpson et al., 2015). Visit Addgene's MIcrobiology Pages The problem: Host binding proteins...

  2. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

    Type
    Blog Post
    Published
    May 17, 2016, 2:30 p.m.
    .... Current table columns are target gene, species, sequence, Addgene plasmid ID (if we have one associated...reviewed articles. If you’re targeting your favorite gene with CRISPR, using one of these validated gRNAs ...is worth trying alternate names, particularly for gene names. If you find a sequence that will work for... planning an experiment to performing one.  At Addgene, over 120 labs have deposited CRISPR reagents, ...already been shown to work in CRISPR experiments. Addgene’s dedication to resource sharing has led us to develop... is welcomed for validated gRNAs that are not generated from a plasmid. If you, for example, prefer to...Sharing. 2016. In press. Additional Resources on the Addgene Blog Learn How to Design Your gRNA Use the CRISPR...

  3. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...throughput studies of optogenetics.  Common opsins used in zebrafish Optogenetics functions through opsins....  Additional resources on the Addgene blog Find all our optogenetics blog posts Read blog posts about... viral vectors Subscribe to the Addgene blog Resources on Addgene.org Learn about our ready-to-use viral...Optogenetics, the use of light sensitive proteins (opsins) to manipulate cell activity, enables researchers...escape reflex. These traits, coupled with short generation times and high fecundity, make zebrafish ideal...are light sensitive proteins. Opsins used in optogenetic studies are not native to vertebrates and are...swimming  Researchers at the Isacoff lab applied optogenetics in zebrafish larvae to identify new neurons ...

  4. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    Published
    June 13, 2019, 12:19 p.m.
    ...content from Addgene, subscribe to Addgene’s YouTube Channel. Additional resources on the Addgene blog Learn...about Addgene's Lab Tips video series Resources on Addgene.org Find lab protocols on the Addgene website...Since rolling out our viral service in 2016, Addgene has shipped thousands of AAV and lentiviral preps... purity of all AAV viral lots. Aligning with Addgene’s mission to “accelerate research and discovery ...improve your experiment results! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient...calculations on how to prepare a plasmid stock used to generate a standard curve, visit the AAV Titration by qPCR...website Watch Addgene's video collection ...

  5. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    Published
    June 16, 2017, 2 p.m.
    .... Along with our role as a plasmid repository, Addgene aims to be a vital educational resource for scientists...mind, we are very excited to debut our brand new Addgene Videos page! Videos are a powerful tool for communicating...or to answer any questions you might have about Addgene. The page is divided into three main sections: ... real Addgenies who have a variety of roles at Addgene.  You’ll hear all about their career paths, future...feature screen-capture footage to help you navigate Addgene’s vast online resources. Our most popular How-To...stage of the selection and checkout process on Addgene’s website: We’ll be continuously updating this...comments section below! Additional Resources on the Addgene Blog 5 Science Rap Videos and Their Creator: Tom...

  6. Addgene's a Nonprofit? Nonprofit Awareness Day 2017

    Type
    Blog Post
    Published
    June 5, 2017, 3:19 p.m.
    ...their mission. Addgene is fortunate in that we are able to fulfill our mission by generating revenue through... is a bit misleading. Nonprofits can generate revenue (as Addgene does through materials distribution)... at Addgene If you’re ever curious what specifically drives the decision making process at Addgene, just...in the community Addgene fosters a culture of respect, collaboration, and fun Addgene believes there is... on the Addgene Blog Learn about Our Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports...to Our Interview with Addgene Board Member, Connie Cepko Resources on Addgene.org Learn More About Our...Our Mission Meet the Addgene Leadership Team Get the Latest News from Addgene ...

  7. Addgene's Top Blog Posts from 2020

    Type
    Blog Post
    Published
    Dec. 29, 2020, 2:15 p.m.
    ...to highlight some of the top articles from the Addgene blog this year. Spoiler alert: many of the top ...subset of the fluorescent protein family and they generate color! They've been adapted for many molecular...SARS-CoV-2, including many with plasmids deposited at Addgene! 2. DNA Purification Without a Kit by Beth Kenkel...

  8. Addgene’s First AAV Data Hub Challenge

    Type
    Blog Post
    Published
    Sept. 20, 2022, 1:15 p.m.
    ...We're going virtual for Addgene’s first AAV Data Hub Challenge, now taking place Dec. 6, 2022! Share ...Anyone can submit data from their CLOVER lab or Addgene AAV experiments to the Data Hub. Submit your data...travel award. All participants will receive an Addgene t-shirt.  Our judges, Drs. Connie Cepko, Ian Wickersham...

  9. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ... time or location can be critical when, say, the gene is essential during development and a knockout is...the Addgene blog Viral Vectors 101: Optogenetics Additional resources on Addgene.org Optogenetics Guide... The Chow Lab has also generated a variety of membrane-recruitment optogenetic tools for manipulating ...a full toolbox and workflow for generating new Rho GTPase optogenetic systems (Berlew et al., 2022). Stopping...LOV, CRY2-CIB, and other optogenetic modules? Or want to find more optogenetic tools in our collection?...collection? Check out our Optogenetics Guide or browse our collections of optogenetics plasmids – and keep an eye...Optogenetics gives you the power to control cells or organisms with the flip of a switch. You may be ...

  10. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...a second plasmid will be needed to introduce the gene of interest containing the chosen codon that will...limiting. It may be time, then, to look towards genetic code expansion (GCE): techniques in which non-canonical...essentially those needed to make site-directed mutagenesis, many researchers new to GCE aren’t able to successfully...added phosphoserine. A community resource Like Addgene, GCE4All is a community resource which depends ...researchers. One way of doing that is by using Addgene’s Blue Flame award to identify highly requested ...the plasmids and bacterial strains (available at Addgene for GCE work. Plus, keep an eye on our blog – we...., Griffith, M. C., & Schultz, P. G. (1989). A General Method for Site-specific Incorporation of Unnatural...
Showing: 311 - 320 of 928 results