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TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....not at all obvious, intuitive, or logical to a layperson. When you’re communicating ideas that you think... Rachel Leeson...
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Antibodies 101: Affinity Reagents
TypeBlog PostPublishedApril 14, 2022, 1:15 p.m....entirely new affinity reagent. Fig. 1: A comparison of an antibody (left) to several alternative ... Rachel Leeson... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....world of research affinity reagents, and for good reason. However, sometimes you want a tool that is a little...Variable (scFv). Size: ~25 kDa. Figure 2: Comparison between a full IgG antibody and common antigen... -
Transferable Skills Guide: Public Speaking
TypeBlog PostPublishedApril 20, 2023, 4 p.m....talk. That moment taught me an incredibly valuable lesson: Your presentation will be better if you take time... Rachel Leeson... -
The time and cost required to make a plasmid
TypeBlog PostPublishedMarch 23, 2023, 1:15 p.m....written by Jennifer Tsang and updated by Rachel Leeson. Have you ever wondered how long it takes to make...if budget is a concern. The faster methods, like Gibson assembly, are typically more expensive, so you’... -
Hot Plasmids April 2018 - Protein Degradation, Nanoscopy, FIRE-Cas9, and Yeast Expression Tools
TypeBlog PostPublishedApril 23, 2018, 2 p.m....the first author of this article, Dr. Douglas Richardson, recently contributed a relevant post to the ...Addgene blog. Find the SRpHi Biosensors here. Richardson et al. Nat Communications. 2017. PubMed PMID:... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....cracks. In this post we’ll highlight some of the lessons we’ve learned over the years with the hope that...interesting biotechnology code.There are valid technical reasons to choose a non-Python stack, but if you're working... -
Plasmids 101: Golden Gate Cloning
TypeBlog PostPublishedAug. 27, 2015, 2:30 p.m....cloning methods. Exonuclease-based methods like Gibson assembly require 20-40 bp of homology at the ends...using an alternative method like Gateway cloning or Gibson assembly. Another important consideration is the... -
Working with Nuclear Receptors
TypeBlog PostPublishedJuly 15, 2014, 4:03 p.m....making them available to all researchers for a reasonable fee. We at NURSA recognize the importance of ...at www.nursa.org. Bert O’Malley, M.D., is Tom Thompson Professor and Chair of the Department of Molecular... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....consists of highly useful vectors compatible with Gibson and Saptrap cloning systems to generate AID* tagged...degradation. New from the viral service By Jason Nasse We regularly add new viral aliquots from our...