We narrowed to 798 results for: Arc
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TypeBlog PostPublishedApril 9, 2019, 1:13 p.m....components of the chimera are encoded by DNA. Since researchers can generate almost any DNA sequence in the way...In addition to measuring stickiness, several researchers have also collected data on fluorescent protein...
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Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog PostPublishedOct. 5, 2021, 1:15 p.m....we decided to write this quick guide to help researchers choose the probe for their needs. You’ll find...biosensors, and optogenetic tools. Follow her recent research @daria_gift_shch. References and resources... -
Antibodies 101: Beyond Surface Labeling
TypeBlog PostPublishedJune 27, 2024, 1:15 p.m....permeabilized membrane to enter cells. Depending on the research question and downstream application, one or several...depending on the application. Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard... -
Antibodies 101: Introduction to Gating in Flow Cytometry
TypeBlog PostPublishedApril 4, 2024, 1:15 p.m....used for virtually all kinds of experiments and research fields, for any experiment, your first three gates...visualize four populations at once. Paul Heisig is a Research Associate in the lab of Arlene Sharpe at Harvard... -
Hot Biosensors 2022: Year-End Roundup
TypeBlog PostPublishedDec. 6, 2022, 2:15 p.m....mice under a variety of behaviors, opening up new research directions that were inaccessible with earlier...hope these tools will be useful in your future research, and we’re looking forward to even more innovation... -
CRISPR 101: Validating Your Genome Edit
TypeBlog PostPublishedNov. 3, 2022, 12:15 p.m....sequence trace decomposition. (2014) Nucleic Acids Research Volume 42, Issue 22, Page e168. https://doi.org...template-directed CRISPR-Cas9 editing. (2018) Nucleic Acids Research Volume 46, Issue 10, Page e58. https://doi.org... -
Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping
TypeBlog PostPublishedDec. 2, 2019, 2 p.m....PMC6126969. Che, Yonglu, and Paul A. Khavari. "Research Techniques Made Simple: Emerging Methods to Elucidate...proximity-based labeling methods for interactome mapping. F1000Research, 8. ... -
Site Directed Mutagenesis by PCR
TypeBlog PostPublishedAug. 2, 2016, 2:30 p.m....Blogger Kristian Laursen! Kristian Laursen is a researcher at Cornell University. References 1. Breslauer... for site-directed mutagenesis." Nucleic acids research (2014): gku1189. PubMed PMID: 25399421. PubMed... -
Plasmids 101: Common Lab E. coli Strains
TypeBlog PostPublishedNov. 7, 2014, 2:56 p.m....of E. coli currently available – a quick Google search suggests there are hundreds. This only includes... of B strain is a bit more convoluted due to researchers sharing and renaming samples throughout history... -
Viral Vectors 101: AAV Variables That Matter
TypeBlog PostPublishedJune 1, 2023, 1:15 p.m.... specificity of GFAP transgenes. Neurochemical Research, 29(11 SPEC. ISS.), 2075–2093. https://doi.org...mouse and macaque cerebral cortex. Neuroscience Research, 93, 144–157. https://doi.org/10.1016/j.neures... -
Mycoplasma Contamination: Where Does It Come From and How to Prevent It
TypeBlog PostPublishedOct. 8, 2020, 1:15 p.m....contributed by guest blogger, Kaustubh Kishor Jadhav, a Research Assistant at MGMs Institute of Biosciences and...Kishor Jadhav! Kaustubh Kishor Jadhav, MSc is a Research Assistant at the MGMs Institute of Biosciences... -
Transferable Skills Guide: Teamwork
TypeBlog PostPublishedNov. 28, 2017, 2 p.m....A recent survey of PhDs found that many researchers feel that they lack formal training in a variety ...out to fill this gap by both highlighting that researchers do learn MANY transferable skills while working... -
Plasmids 101: Modular Cloning Applications and Kits
TypeBlog PostPublishedMay 14, 2024, 1:15 p.m....amounts of pure product in an affordable way, researchers test many different combinations of promoters...testing CRISPR tools, it should be easy enough for researchers to expand the Fragmid toolkit to facilitate other... -
A Needle in a Base-Stack: Cas9 Structural Biology
TypeBlog PostPublishedJune 4, 2024, 1:15 p.m....tightly to DNA even after cleaving it, so many researchers think the enzyme needs cellular recycling factors...valuable laboratory tool are still an area of active research. New details are still being uncovered, and different... -
Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2: Reprogramming and Transdifferentiation
TypeBlog PostPublishedNov. 6, 2018, 1:12 p.m.... transdifferentiation and reprogramming give researchers the ability to convert a differentiated cell ...our Stem Cell Pages Find Viral Vectors for Your Research Find Plasmids from the Yamanaka Lab ... -
Deep Dive: qPCR
TypeBlog PostPublishedSept. 29, 2022, 1:15 p.m....sample=control Gentle, et. al No Raw Data researcher defines log-linear phase Liu and Saint No...Scorpion primers to mutation detection, Nucleic Acids Research, Volume 28, Issue 19, 1 October 2000, Pages 3752... -
CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More
TypeBlog PostPublishedSept. 28, 2020, noon.... Here’s some others. SoxS based CRISPRa In the search for a CRISPR activation method in E. coli, Jesse...an engineered CRISPR-Cas system. Nucleic Acids Research 41:7429–7437 . https://doi.org/10.1093/nar/gkt520... -
Troubleshooting and Optimizing a Western Blot
TypeBlog PostPublishedSept. 17, 2024, 1:15 p.m....Blot Doctor page, or robust discussions, like ResearchGate’s western blot forum. Blocking Don’t skimp on...dots, lines, smudges, etc...) Technical errors Search online/ask an expert Review protocol videos... -
Addgene Launches Partnership with IPI
TypeBlog PostPublishedJune 13, 2023, 1:15 p.m....(IPI). Together, we’ll be working to provide researchers with a collection of IPI’s well-validated and... -
Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!
TypeBlog PostPublishedApril 8, 2021, 7:25 p.m....can track the number of plasmids right below the search bar and more stats further down the page....