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We narrowed to 898 results for: CAN

Showing: 621 - 640 of 898 results

  1. Stepping into a Virtual Internship: My Experience at Addgene

    Type
    Blog Post
    Published
    Aug. 13, 2020, 1:21 p.m.
    ... our mindsets and needs can change. Having been at Addgene for a month, I can say with certainty that ...options is being an overthinker. Being an overthinker can make me scared of taking risks, afraid of leaping... I introduced the team to a new design program, Canva, and I was able to create cute and on-brand templates...

  2. New FLIS Plasmid (April Fools 2022)

    Type
    Blog Post
    Published
    April 1, 2022, 1:15 p.m.
    ...incorporates a bioluminescence protein into the chitin that can be activated only by a specific frequency. Once activated...eye, at regular intervals. This innovation system can help fly labs everywhere find and re-contain any ...well, annoy. Fly-related Issues in the Lab A significant drawback of working with fruit flies is their...

  3. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ...genome that can cause disruption of functional genes at the site of insertion and which can potentially...cells that are otherwise difficult to obtain. These can then be used to perform cell therapy and to model...is sold at $11,000 at ThermoFisher Scientific and can be used for only 6-8 reprogramming reactions. Another...terms of hiPSC colonies, nearly 16 hiPSC colonies can now be obtained from just 1 ml of peripheral blood...

  4. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

    Type
    Blog Post
    Published
    July 29, 2025, 1:15 p.m.
    ...requirements expand the number of genomic sites that can be targeted with TnpB, offering access to regions... TnpB is a much smaller genome editing tool that can be efficiently packaged into viral vectors. Even .... In our recent study, we demonstrated that TnpB can also function as an efficient gene activator when...et al., 2024). Additionally, we showed that TnpB can be used to simultaneously target multiple genes, ...efficiency of guides are currently unavailable, you can use TEEP — a tool based on animal data. Step 2...

  5. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type
    Blog Post
    Published
    April 2, 2019, 1:44 p.m.
    ...communities have a little more leeway in what they can name a gene and many of the interesting ones come...also have many plasmids in our repository that you can use to study these genes! Groucho, a transcriptional...Addgene has several plasmids that contain SHH, you can find them all here! The most popular SHH plasmid ...Sleeping Beauty is a synthetic transposon system that can be used to insert specific pieces of DNA into vertebrate... resurrecting this transposon system? Scientists can use Sleeping Beauty transposons as a non-viral system...deposited by the Shivdasani Lab from the Dana Farber Cancer Institute! Sonic Hedgehog, an important regulator...

  6. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ...This light-induced excitation can take place in a tissue culture dish but can also happen in real-time inside...still require fiber-optic cannula implantation, but altering ChRgers so they can be activated with tissue-penetrating...ChRgers, however, have a higher conductance, so they can still depolarize neurons at lower MOIs. Figure...the VTA is located in the midbrain, fiber-optic cannulas were still stereotaxically implanted into the ...mice’s skull near the M2 and secure a fiber optic cannula to the skull, leaving the brain intact. When the...

  7. Supporting Rare Disease Research with the 2020 BeHEARD Awards

    Type
    Blog Post
    Published
    Sept. 8, 2020, 2 p.m.
    ...channels typically expressed on other cell types can compensate for or bypass the dysfunction of another...mammalian cells to identify any solute channels that can compensate for the defect caused by SLC6A8 deficiency...hypothesized that this is abnormally deregulated in TS and can lead to cardiac developmental defects. Eight genes...example, GPCRs and their downstream G protein partners can regulate ion channels in the brain.  “I have a long...genes from the X chromosome are expressed at significantly reduced levels in women with TS (Trolle et al...vectors from Addgene, the lab will express these candidate genes in TS iPSCs to identify whether any rescue... use CRISPR plasmids to generate deletions of candidate genes in euploid iPSCs. “The BeHEARD Addgene reagents...

  8. CRISPR 101: Cas9 vs. The Other Cas(s)

    Type
    Blog Post
    Published
    Dec. 8, 2022, 2:15 p.m.
    ...single stranded DNA degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one...of the size) and does not require a tracrRNA (it can self-process pre-crRNA into crRNA). The size and ...likely due to the lack of collateral cleavage). You can read about Cas7-11 in our blog post on its development...are worth taking a look at. Choosing the right Cas can really put your experiments Nsync.   References ...enzymes outlined below as well. Cas12a also has significant therapeutic promise due to the fact that it is...target sequence Similar to Cas12, it is also significantly smaller in size than traditional Cas9. Another... not display collateral RNA cleavage and is significantly less toxic to mammalian cells than Cas13 (likely...

  9. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

    Type
    Blog Post
    Published
    May 5, 2015, 3:54 p.m.
    ...it works for me. I can often remember which paper I need to open, but when I can’t it’s an easy search...scientific literature are Google Scholar and PubMed. You can search keywords, titles, authors, year, narrow by...(relevance, dates etc). The links for the titles can generally take you to the full text of the article... same conclusion. Personally, I like papers that can end with some sort of model (cartoons are appreciated...Set aside some time each day or week to at least scan the titles of what’s going on in your research area...as soon as they are available. Give them a quick scan for interesting key words and download what seems...

  10. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ...we believe that by coming together, we can create a significant impact on both clinical care and public...collaboration as the best way that the scientific community can make progress towards understanding this disease....resources nationally and internationally such that we can advance COVID-19 research to hopefully help guide... COVID-19 Resources Canada site. To date, the portal has recruited 3,218 Canadian volunteers, and features...nationally and internationally COVID-19 Resources Canada is a database that provides information about reagents...research into COVID-19. While this program is based in Canada, much of the information is applicable to researchers...

  11. What's New in CRISPR - March 2020

    Type
    Blog Post
    Published
    March 10, 2020, 1:15 p.m.
    ... the complex enters the nucleus where dCas9-Mxi1 can repress gene expression. In the dark, the NLS is ...expression of dCas9). The lab demonstrated the system can also be used to downregulate multiple genes at once...modifiers such as TET1 with inactive Cas enzymes can alter gene expression via acetylation or demethylation...

  12. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    Published
    Jan. 21, 2020, 2:30 p.m.
    ... particular disease, cell type or drug treatment can browse through screens performed in relevant cell...otherwise be hard to identify. Figure 2: Users can cross-interrogate screens from the literature for...original publication via its PubMed identifier, which can also be used to search the entire CRISPR database... the term "breast cancer" returns 40 screens performed in 26 different breast cancer cell lines. Screenshot...The analytical method for score assignment and significance thresholds used by the authors to determine ...

  13. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

    Type
    Blog Post
    Published
    June 19, 2018, 1:38 p.m.
    ...arg1, each ARG’s distinct ultrasound fingerprint can be detected. Bourdeau et al calls this method acoustic...experiments because it’s a probiotic strain that can colonize the mammalian intestinal tract and has been...somewhere in the mouse abdomen. arg1 signal, however, can be overlaid onto anatomical ultrasound imaging to...

  14. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ... in-person, you helped us understand how Addgene can better serve the global scientific community. As ...Almost any AAV transfer plasmid in our repository can now be packaged on demand in five distinct serotypes... — ‌and reinventing our search experience so you can find exactly what you need, faster. We’re also building...tell only part of the story. This year, we made significant strides in making science more accessible and...

  15. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ...to biosensor creation, and custom FRET biosensors can be created using the cpFRET kit created by Olivier...enzymatic activity or phosphorylation over time. You can also use biosensors to follow processes such as protein...targeting signal or a transmembrane domain, biosensors can be targeted to a particular cellular compartment ...

  16. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ...spending time outdoors in nature or visiting museums can be excellent methods to detach from the constant ...is not just a stroke of luck; it's a mindset that can lead to unexpected discoveries and innovative solutions...serendipity in your lab research and PhD studies can enrich the depth and creativity of your work, while...

  17. Same Addgene, New Look - Why We Redesigned Our Homepage & Mascot

    Type
    Blog Post
    Published
    Dec. 5, 2019, 1:49 p.m.
    ...planned. Blugene also has full arms so that they can hold and hug items closer and do so more animatedly...with a variety of science-inspired friends. As you can see on the homepage, Blugene is now accompanied by... viral vector for your next experiment, that you can find what you need and feel happy and inspired to...

  18. The Blue Flame Award: Celebrating Addgene's Most Requested Depositors

    Type
    Blog Post
    Published
    Nov. 14, 2016, 3:30 p.m.
    ...deposited in Addgene!” As you can imagine, answering plasmid requests can be a time-consuming chore for...benefits of sharing plasmids with us. One way we can gauge if people are happy depositors is if they deposit...

  19. Addgene at the ASM Microbe 2016 Conference

    Type
    Blog Post
    Published
    July 7, 2016, 2:30 p.m.
    ...diverse and exciting field and to find out how we can serve them better. Here’s a little bit of what we...into science education and outreach ourselves, we can a learn a lot from these ASM pros and hope to leverage...materials! Resources on the Addgene Blog Learn How You Can Use Addgene's Microbiology Pages Read Our Post on...page and, just a few weeks ago, we attended the American Society for Microbiology's annual meeting (ASM...

  20. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    Published
    Aug. 4, 2015, 2:30 p.m.
    ...made the accidental discovery that laser treatment can photoconvert GFP from green to RED! This simple technique...photoconvertible, new studies of spatiotemporal dynamics can be undertaken using available GFP lines. Like many...compartments. Sattarzadeh et al. have shown that GFP can be rapidly and efficiently photoconverted in plant...
Showing: 621 - 640 of 898 results