We narrowed to 911 results for: CAN

...expresses the HIV gag, pol, rev, and tet genes. It can be used with 2nd or 3rd generation lentiviral vectors... proteins determine which types of cells a virus can infect. The VSV-G envelope protein enables this virus...BbsI restriction site, a pair of annealed oligos can be cloned to create a chimeric guide RNA. Find pX330...producing lentiviral and MuLV retroviral particles. It can be used with packaging plasmids such as pCMV-dR8.2...

...mentoring program at your organization, contact me! I can provide a step-by-step, how-to guide to get one started...same time Broadens the network of the participants Can be a lot of fun! Form your own peer mentoring group...interested and willing to commit to the group. The group can be one gender and career stage (e.g. all women, all... mixed for one or both of these criteria. If you can, include people from different labs, departments ... from peer groups meeting at both the Fox Chase Cancer Center and Brandeis University. 5 easy steps to...meet regularly no matter what, even if one person can’t make it. One of the biggest group mentoring predictors...

... with the other 11 presents!)  Can't get enough of CRISPR? Neither can we! Subscribe below to get updates...CRISPR in 2021 and useful resources, fear not! You can find all of them here.  On the first day of CRISPR...

...interested in engaging with us on social media, you can find us on Bluesky: @addgene.bsky.social; or @addgene...associated with an official Addgene account. You can reach out to us directly via email at [email protected]... suggestions in the comments.  X has moved significantly away from the functionality and features that...this reason, our timeline has accelerated. We significantly reduced posting on November 20, 2023, and as...

...achieving high yields for your viral vector preps can be a challenge. You probably know yield is influenced...plasmids for our AAV Viral Vector Service, and you can use them in your lab too! To date, we've produced...AAV or try our Packaged on Request service so you can spend less time producing virus and more time doing...lysate and living cells, so transcriptome states can be recorded with cell identity preserved. The team... from that population after treatment. TimeVault can be delivered as a single construct or stably transfected...libraries aim to increase the quality of data you can get from your CRISPR screen (Figure 5). First, the...TimeVault to show that a subpopulation of PC9 lung cancer cells had a distinct transcriptome that was associated...

...) are flanked by unique restriction sites so you can swap sequences effortlessly.  We built these plasmids...plasmids for flexibility and speed, so you can design, test, and innovate faster than ever. Plus, we've...fused to a shortened R2Tocc transposon protein, and can "retarget" the transposon to new loci in the genome...recruitment and nicking, producing STITCHR. STITCHR can make a wide variety of genomic edits — single-base... proteins with biotin. The biotinylated proteins can then be isolated and identified with tools like mass...gene expression to specific cell types in the brain can be a challenging — but critical — factor for successful...the IPI Glypican Collection here! Figure 3: General glypican domain structure. Glypicans are proteoglycans...

...published concurrently in Science have shown CRISPR can treat a genetic disease in a postnatal mouse model...another approach. Since shorter forms of dystrophin can still be functional, exon skipping is a good option...culture, but Ran et al. have shown that CRISPR and AAV can be used together for postnatal genome editing in ...strategies for Duchenne muscular dystrophy. gRNAs can be designed to flank a single or multiple exons that...’d like to treat with CRISPR. As seen above, DMD can be treated with “one-size-fits-most” NHEJ-mediated...DMD. If the safety and efficacy of this approach can be optimized, DMD could become one of the first diseases...science.aae0169 2. Long C, Amoasii L, Mireault AA, McAnally JR, Li H, Sanchez-Ortiz E, Bhattacharyya S, Shelton...

... our mindsets and needs can change. Having been at Addgene for a month, I can say with certainty that ...options is being an overthinker. Being an overthinker can make me scared of taking risks, afraid of leaping... I introduced the team to a new design program, Canva, and I was able to create cute and on-brand templates...

...incorporates a bioluminescence protein into the chitin that can be activated only by a specific frequency. Once activated...eye, at regular intervals. This innovation system can help fly labs everywhere find and re-contain any ...well, annoy. Fly-related Issues in the Lab A significant drawback of working with fruit flies is their...

...requirements expand the number of genomic sites that can be targeted with TnpB, offering access to regions... TnpB is a much smaller genome editing tool that can be efficiently packaged into viral vectors. Even .... In our recent study, we demonstrated that TnpB can also function as an efficient gene activator when...et al., 2024). Additionally, we showed that TnpB can be used to simultaneously target multiple genes, ...efficiency of guides are currently unavailable, you can use TEEP — a tool based on animal data. Step 2...

...communities have a little more leeway in what they can name a gene and many of the interesting ones come...also have many plasmids in our repository that you can use to study these genes! Groucho, a transcriptional...Addgene has several plasmids that contain SHH, you can find them all here! The most popular SHH plasmid ...Sleeping Beauty is a synthetic transposon system that can be used to insert specific pieces of DNA into vertebrate... resurrecting this transposon system? Scientists can use Sleeping Beauty transposons as a non-viral system...deposited by the Shivdasani Lab from the Dana Farber Cancer Institute! Sonic Hedgehog, an important regulator...

...genome that can cause disruption of functional genes at the site of insertion and which can potentially...cells that are otherwise difficult to obtain. These can then be used to perform cell therapy and to model...is sold at $11,000 at ThermoFisher Scientific and can be used for only 6-8 reprogramming reactions. Another...terms of hiPSC colonies, nearly 16 hiPSC colonies can now be obtained from just 1 ml of peripheral blood...

...This light-induced excitation can take place in a tissue culture dish but can also happen in real-time inside...still require fiber-optic cannula implantation, but altering ChRgers so they can be activated with tissue-penetrating...ChRgers, however, have a higher conductance, so they can still depolarize neurons at lower MOIs. Figure...the VTA is located in the midbrain, fiber-optic cannulas were still stereotaxically implanted into the ...mice’s skull near the M2 and secure a fiber optic cannula to the skull, leaving the brain intact. When the...

...channels typically expressed on other cell types can compensate for or bypass the dysfunction of another...mammalian cells to identify any solute channels that can compensate for the defect caused by SLC6A8 deficiency...hypothesized that this is abnormally deregulated in TS and can lead to cardiac developmental defects. Eight genes...example, GPCRs and their downstream G protein partners can regulate ion channels in the brain.  “I have a long...genes from the X chromosome are expressed at significantly reduced levels in women with TS (Trolle et al...vectors from Addgene, the lab will express these candidate genes in TS iPSCs to identify whether any rescue... use CRISPR plasmids to generate deletions of candidate genes in euploid iPSCs. “The BeHEARD Addgene reagents...

...single stranded DNA degradation. Cas3 DNA digestion can proceed up to multiple kilobases, but only in one...of the size) and does not require a tracrRNA (it can self-process pre-crRNA into crRNA). The size and ...likely due to the lack of collateral cleavage). You can read about Cas7-11 in our blog post on its development...are worth taking a look at. Choosing the right Cas can really put your experiments Nsync.   References ...enzymes outlined below as well. Cas12a also has significant therapeutic promise due to the fact that it is...target sequence Similar to Cas12, it is also significantly smaller in size than traditional Cas9. Another... not display collateral RNA cleavage and is significantly less toxic to mammalian cells than Cas13 (likely...

...it works for me. I can often remember which paper I need to open, but when I can’t it’s an easy search...scientific literature are Google Scholar and PubMed. You can search keywords, titles, authors, year, narrow by...(relevance, dates etc). The links for the titles can generally take you to the full text of the article... same conclusion. Personally, I like papers that can end with some sort of model (cartoons are appreciated...Set aside some time each day or week to at least scan the titles of what’s going on in your research area...as soon as they are available. Give them a quick scan for interesting key words and download what seems...

... away from any known Cas protein. This construct can be used with the AI generated synthetic guide RNA...generate a tagged fusion protein. Fusion proteins can then be covalently labeled with a molecule such as...for use in mammalian cells as well as those that can be used in bacterial cells for protein purification... available to industry scientists has grown significantly over the past few years, we wanted to highlight...distributes over 30 recombinant antibodies targeting glypicans and integrins.  The Stasevich Lab's Frankenbody...

...arg1, each ARG’s distinct ultrasound fingerprint can be detected. Bourdeau et al calls this method acoustic...experiments because it’s a probiotic strain that can colonize the mammalian intestinal tract and has been...somewhere in the mouse abdomen. arg1 signal, however, can be overlaid onto anatomical ultrasound imaging to...

...to biosensor creation, and custom FRET biosensors can be created using the cpFRET kit created by Olivier...enzymatic activity or phosphorylation over time. You can also use biosensors to follow processes such as protein...targeting signal or a transmembrane domain, biosensors can be targeted to a particular cellular compartment ...

... in-person, you helped us understand how Addgene can better serve the global scientific community. As ...Almost any AAV transfer plasmid in our repository can now be packaged on demand in five distinct serotypes... — ‌and reinventing our search experience so you can find exactly what you need, faster. We’re also building...tell only part of the story. This year, we made significant strides in making science more accessible and...