We narrowed to 803 results for: Cre

...researcher experiences and that it often provides incredibly valuable insight into one’s work. As a community...information can be cited, tracked, and appropriately credited, broadening the knowledge base of AAV vector performance...

...This post was contributed by guest blogger Tessa Cressey. The highly pathogenic Ebola virus belongs to the...various viral components play in disease. Tessa Cressey is a PhD Student in the labs of Dr. Elke Mühlberger...

...PAM site, Cpf1 cleaves in a staggered fashion, creating a 5 nucleotide 5’ overhang 18-23 bases away from...mediated editing, as Kleinstiver et al. saw no decrease in Cpf1 activity with 4-6 base deletions at the...

...EVER confirmed this with me). While things were incredibly busy in those early years, the founders knew ...aspect of Addgene. As our size has drastically increased, it would have been a tight squeeze for all of...

... of transcriptional regulation by an enhancer. Created with BioRender.com.   Developing AAV enhancer...just that, and their painstaking efforts towards screening thousands of enhancer sequences has culminated...

...gene disruption in Mycobacterium abscessus, an increasingly common human pathogen notorious for its high...adhesion to a PILATeS-functionalized coverslip creates mechanical tension, and the luminescence colocalizes...

...released Viral Vectors 101 2nd edition — I was incredibly excited when we picked our viral vector service...GCaMP6. It could be a matter of the size of the incremental improvements between generations: jGCaMP8 allows...

...the presence of your targeted nucleic acid. To increase the sensitivity of the assay, targeted DNA or ...non-specific (trans) ssDNA and harnessed this ability to create a DNA detection platform called DETECTR. How does...

... you when you need it. The opportunity to be creative and think critically. One of the very first times...defined as sitting down at a computer and just creating, which for many scientists is the most enjoyable...

...microbes would be affected (possibly killed), thus increasing the initial microbe’s chances of survival. This...antibiotic-free, farmed meats. We can focus on creating new antibiotic therapies, and investigate more...

...trolling: The art of deliberately, cleverly, and secretly pissing people off, usually via the internet, ...excellent. Finally, as I said in the AMA, it’s incredibly important to find good mentors and incentivize...

...line you will need to grow this initial stock and create a set of vials of ‘Master’ stock, which can be ...thawed, it is time to reorder this cell line to create new Master and Working stocks to work from. The...

...subtle and may include reduced saturation density, decreased rates of growth, or clumping. To ensure the best...Viral Vector Elements Learn How to Do Genome-Wide Screens with CRISPR Lentiviral Vectors Additional Resources...

...Plasmid A is the decreased expression of Gene X. Ergo the positive control(s) should decrease the expression...

...interest is toxic. To combat this, scientists have created synthetic promoters, which typically include some...with somewhat weak expression. lacIq mutation increases expression of the repressor 10x, thus tightening...

...A., Tao, Y. A., Duby, J. E., Steinbeck, B. J., McCreary, J., Pierce, S. E., … & Liu, D. R. (2024). Efficient...variety of DNA-targeting proteins, so the team created versions using dCas9, zinc finger proteins, and...

... S65T) in the original jellyfish GFP (AvGFP) to create enhanced GFP (EGFP), a variant with improved brightness...fluorescent probes, recent engineering efforts have increased the brightness of these genetically encoded light...

...completing the research will advance the field, create a new field, impact disease treatment, etc., again... His interests include the quality control of secretory proteins in the Endoplasmic Reticulum, optimization...

...plus one additional split vector strategy for increasing the size of the AAV donor template for CRISPR-mediated... AAV vector approaches Split vector approaches increase the size of the gene delivered by splitting the...

...immunofluorescence and indirect immunofluorescence work. Image created with BioRender.com   Indirect IF is a much ...with key considerations about each step. Image created with BioRender.com   The first couple of steps...