We narrowed to 694 results for: EED

1. RNA Extraction Without A Kit

   Type

   Blog Post

   Published

   March 24, 2020, 1:15 p.m.

   ...foremost, be mindful of your extractions- if you need really clean RNA, it’s important to make sure that...

2. Hot Plasmids - March 2020 - base editors, GEVI, MoClo, and optogenetics

   Type

   Blog Post

   Published

   March 3, 2020, 2:15 p.m.

   ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

3. Giving gRNAs a Facelift - Synthetic and Beyond

   Type

   Blog Post

   Published

   Oct. 20, 2022, 1:15 p.m.

   ...Genome Editing, 28 (2021). https://doi.org/10.3389/fgeed.2020.617910 Zou, R., Liu, Y., Wu, B., et al. Cas9...

4. All in a Twist: dsRNA

   Type

   Blog Post

   Published

   Feb. 2, 2023, 2:15 p.m.

   ...duplexed RNA DNA:RNA hybrids Sometimes ssRNA just needs a buddy, and even DNA will suffice. This can result...

5. Plasmids 101: CcdB - The Toxic Key to Efficient Cloning

   Type

   Blog Post

   Published

   Dec. 8, 2016, 3:30 p.m.

   ...variety of useful resources and protocols! We invite feedback from all members of the research community to ...

6. BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

   Type

   Blog Post

   Published

   June 26, 2018, 1:33 p.m.

   ...Arg) amino acids more frequently than any other. Indeed, nearly 20% of the single missense mutant alleles...

7. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

   Type

   Blog Post

   Published

   May 15, 2018, 12:43 p.m.

   ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

8. Split-BioID: An Improved Method for Studying Protein-Protein Interactions

   Type

   Blog Post

   Published

   May 1, 2018, 1:57 p.m.

   ...Another potential limitation of Split-BioID is its speed of labeling. BioID requires 6-24 hours for tetracycline...

9. Plasmids for Endogenous Gene Tagging in Human Cells

   Type

   Blog Post

   Published

   April 6, 2017, 1:02 p.m.

   ... the insertion of the GFP tag precisely where we need it, in this example, at the C-terminus of the gene...

10. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ... easier for you to find and use the plasmids you need. If you'd ever like to write about a recent plasmid...

11. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ... a Standard Curve To do that calculation, you’ll need to include a standard curve in your experiment. ...

12. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ...’re just starting out with prime editing, you’ll need to design and optimize a pegRNA for your particular...

13. Plasmids 101: The Wide World of Natural Plasmids

    Type

    Blog Post

    Published

    Oct. 8, 2024, 1:15 p.m.

    ...direct transfer from one cell to another). Bacteria need special fertility genes to be capable of conjugation...

14. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ... single GFP or CFP/YFP-derived FRET pair. If you need to free up crowded optical space for multiplexing...

15. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...sample. The PCR is then performed, optimized as needed, and cleaned up using a standard PCR cleanup kit...

16. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type

    Blog Post

    Published

    Nov. 7, 2017, 1:59 p.m.

    ...deliver gene therapies directly to the cells that need them. Adeno-associated virus (AAV) – based delivery...

17. 5 Tips for Troubleshooting Viral Transductions

    Type

    Blog Post

    Published

    Aug. 11, 2016, 2:23 p.m.

    ...volume. Always remove packaging cell debris before proceeding to ultracentrifugation to avoid contaminating...

18. Plasmids 101: Terminators and PolyA signals

    Type

    Blog Post

    Published

    March 31, 2016, 2:30 p.m.

    ...Plasmids 101 eBook to get all the background you need to start working with plasmids! What are termination...

19. Tips for Titering Your Lentiviral Preps

    Type

    Blog Post

    Published

    March 15, 2016, 2:30 p.m.

    ...fluorescent markers are used as it eliminates the need for antibody staining. Check Out Our In-Depth Fluoresence...

20. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type

    Blog Post

    Published

    Dec. 1, 2015, 3:30 p.m.

    ...datasets and download the custom python scripts needed to discover circRNAs in RNA-seq data. As far as...