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  2. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...multiple replicates and several cell types can strengthen your results. Enrichment or depletion of multiple...

  3. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...C.D. Hojer, J.G. Egen, F. Hellal, F. Bradke, M. Sheng, H.-U. Dodt. (2012). Nature Protocols, 7(11), 1983...

  4. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...included in the search to maximize the alignment length. While this recommendation is not infallible, we...

  6. Getting to Know Expansion Microscopy

    Type
    Blog Post
    Published
    June 20, 2023, 1:15 p.m.
    ...traditional full IgG secondaries, can improve signal strength (Sherry and Stiles, 2022). Fourth, signal continuity...

  7. Simplify Cloning with in vivo Assembly

    Type
    Blog Post
    Published
    Oct. 18, 2018, 12:37 p.m.
    ...temperature (Tm) of around 60 °C for all primers. The length of the homologous region should be around 20 bp...

  10. Plasmids 101: Colony PCR

    Type
    Blog Post
    Published
    May 12, 2016, 2:30 p.m.
    ...Promega Overview for Screening for Recombinants New England Biolabs Overview of Colony PCR Additional Resources...

  13. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...the transfer plasmid. The deletion prevents full-length viral RNA from being produced in target cells and...

  16. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...Overall, writing a review can be overwhelming and challenging. My best advice is don’t overthink it. And to...

  20. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...common microscopy methods. Plus, the luminescence wavelength (460 nm) is shorter than many popular fluorescence...
Showing: 621 - 640 of 673 results