Addgene

We narrowed to 727 results for: HA

Showing: 621 - 640 of 727 results

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Reproducibility for Everyone: Lessons from an Open Science Collaboration

Type

Blog Post

Published

Feb. 28, 2019, 1:34 p.m.

...workshops and lessons learned: Since early 2018 the team has grown to include experts who could help provide new...
Antibodies 101: Buffers, Storage, and Conjugates

Type

Blog Post

Published

Feb. 1, 2022, 2:15 p.m.

...conjugate on the antibody left exposed to light? Has this antibody been left out, gone through a freeze-thaw-freeze...
Viral Vectors 101: Biosensors

Type

Blog Post

Published

July 6, 2023, 1 p.m.

...tools). It’s a good thing, though! Viral delivery has several advantages over other mechanisms. For one...
GCE4All: Making Genetic Code Expansion Accessible

Type

Blog Post

Published

June 15, 2023, 1:15 p.m.

...protocol to work. Or, to quote Ryan Mehl, PhD, GCE has “10x the impact of PCR. But it’s 100x more complicated...
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

Type

Blog Post

Published

Sept. 26, 2024, 1:15 p.m.

...repair work. The key is T4 DNA polymerase, which has 3′→5′ exonuclease activity. T4’s polymerase activity...
Viral Vectors 101: Viruses as Biological Tools

Type

Blog Post

Published

March 21, 2023, 1:15 p.m.

...particles, the functional infection unit. Each particle has a viral envelope, which fuses with the target cell...
Plasmids 101: The protein expression toolbox

Type

Blog Post

Published

Feb. 16, 2023, 7:05 p.m.

...temporary repression of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts! Turn...
Summer SciComm: Preprints

Type

Blog Post

Published

Aug. 16, 2022, 1:15 p.m.

...review is valuable, modern scientific publishing has a number of drawbacks, especially the increasingly...
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Type

Blog Post

Published

Nov. 5, 2019, 1:28 p.m.

...the most widely used CRISPR nuclease, recent work has shown that alternative enzymes may better suit specific...
Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Type

Blog Post

Published

June 2, 2015, 6:06 p.m.

...since its development, CRISPR/Cas9 genome editing has been used to study the effect of gene knockout in...
Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

Type

Blog Post

Published

July 10, 2014, 7:17 p.m.

...Collin’s Lab in 2009. From 2009 to today, Sample6 has changed focus and names, swapped leadership, and ...
Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

Type

Blog Post

Published

Feb. 18, 2014, 4:22 p.m.

...senior mentors available to go around. My advice has been to find 5 interested peers and form your own...
Optogenetics + CRISPR, Using Light to Control Genome Editing

Type

Blog Post

Published

Sept. 3, 2020, 12:15 p.m.

...light is of particular interest since blue light has limited applications in vivo. UV and blue light is...domain from A. sativa (Bubeck et al., 2018). LOV2 has a C-terminal helix that unfolds when stimulated by...
Popular Retroviral Vectors and Their Uses in Scientific Research

Type

Blog Post

Published

July 24, 2018, 12:51 p.m.

...shRNA sequences pLKO (Plasmid #10878) This plasmid has been used by The RNAi Consortium, also known as the...
BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

Type

Blog Post

Published

June 26, 2018, 1:33 p.m.

...brain and muscle development. The Gronostajski lab has generated mice wherein it's possible to delete the...
Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

Type

Blog Post

Published

Feb. 2, 2018, 3:20 p.m.

...Other Resources The National Cancer Institute (NCI) has an internship similar to Susan’s NEI internship. ...
Lighting Up Cell Signaling with Photoswitchable Kinases

Type

Blog Post

Published

July 25, 2017, 1:20 p.m.

... This all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, ...
Choosing the B(right)est Fluorescent Protein: Photostability

Type

Blog Post

Published

June 8, 2017, 2:30 p.m.

... 2008) occasionally and certainly after a set-up has been changed (for instance after replacing a broken...
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

Type

Blog Post

Published

Jan. 11, 2017, 3:30 p.m.

...Future of Research believe that, while the mandate has been removed, institutions should still go ahead ...
Mouse Modeling, Part 1: Genetically Engineered Mice

Type

Blog Post

Published

July 11, 2019, 1:26 p.m.

... nucleases to generate an engineered mouse model has advantages over the injection of a targeting vector...

Showing: 621 - 640 of 727 results

Results per page:

Reproducibility for Everyone: Lessons from an Open Science Collaboration

Antibodies 101: Buffers, Storage, and Conjugates

Viral Vectors 101: Biosensors

GCE4All: Making Genetic Code Expansion Accessible

Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)

Viral Vectors 101: Viruses as Biological Tools

Plasmids 101: The protein expression toolbox

Summer SciComm: Preprints

Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity

Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

Advice for Starting a Biotech Company from a Grad Student turned Entrepreneur

Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

Optogenetics + CRISPR, Using Light to Control Genome Editing

Popular Retroviral Vectors and Their Uses in Scientific Research

BeHeard Award 2018: Diseases of Glycosylation, Arginine Mutagenesis, & Neural Development

Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

Lighting Up Cell Signaling with Photoswitchable Kinases

Choosing the B(right)est Fluorescent Protein: Photostability

Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

Mouse Modeling, Part 1: Genetically Engineered Mice

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