We narrowed to 738 results for: HA
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TypeBlog PostPublishedJune 4, 2019, 1:02 p.m....internal ribosomal entry site and mCherry. If a variant has wild-type levels of abundance, the eGFP will be stable...
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Addgene Depositors Get More Citations
TypeBlog PostPublishedApril 20, 2017, 2:30 p.m....associated plasmids with Addgene. Since then, Addgene has filled over 6,500 requests for these plasmids. While... -
How Addgene Creates Content
TypeBlog PostPublishedNov. 17, 2022, 2:15 p.m.... updating or retiring. CRISPR/Cas9, for example, has gone through rapid change in the past few years. ... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m....this meeting is to _________." Everyone in the room has likely just run in from some other commitment. An... -
Components of CRISPR/Cas9
TypeBlog PostPublishedFeb. 2, 2016, 5 p.m....endonuclease or a variant thereof. This individual protein has all the components necessary to: 1. Bind to a guide... -
Finding and Joining Your Dream Lab
TypeBlog PostPublishedApril 8, 2014, 2:50 p.m....author plus the PI on any paper. Or perhaps, the lab has a reputation for not entering collaborations. Alternatively... -
Viral Vectors 101: Biosensors
TypeBlog PostPublishedJuly 6, 2023, 1 p.m....tools). It’s a good thing, though! Viral delivery has several advantages over other mechanisms. For one... -
GCE4All: Making Genetic Code Expansion Accessible
TypeBlog PostPublishedJune 15, 2023, 1:15 p.m....protocol to work. Or, to quote Ryan Mehl, PhD, GCE has “10x the impact of PCR. But it’s 100x more complicated... -
Viral Vectors 101: Viruses as Biological Tools
TypeBlog PostPublishedMarch 21, 2023, 1:15 p.m....particles, the functional infection unit. Each particle has a viral envelope, which fuses with the target cell... -
Plasmids 101: The protein expression toolbox
TypeBlog PostPublishedFeb. 16, 2023, 7:05 p.m....temporary repression of protein expression. Addgene has a dCas9 KRAB plasmid for transient knockouts! Turn... -
OCaMP: A New Calcium Indicator for Neural Imaging
TypeBlog PostPublishedAug. 19, 2025, 1:15 p.m....pAAV-CMV-OCaMP-WPRE pAAV CMV The Lohman lab has a limited quantity of AAVs that can be requested ... -
Plasmids 101: Sequence and Ligation Independent Cloning (SLIC)
TypeBlog PostPublishedSept. 26, 2024, 1:15 p.m....repair work. The key is T4 DNA polymerase, which has 3′→5′ exonuclease activity. T4’s polymerase activity... -
Summer SciComm: Preprints
TypeBlog PostPublishedAug. 16, 2022, 1:15 p.m....review is valuable, modern scientific publishing has a number of drawbacks, especially the increasingly... -
Antibodies 101: Buffers, Storage, and Conjugates
TypeBlog PostPublishedFeb. 1, 2022, 2:15 p.m....conjugate on the antibody left exposed to light? Has this antibody been left out, gone through a freeze-thaw-freeze... -
Plasmids 101: Transformation, Transduction, Bacterial Conjugation, and Transfection
TypeBlog PostPublishedJune 25, 2019, 12:54 p.m....experiment check out Addgene’s protocol page, which has both protocols and videos for techniques in basic... -
Reproducibility for Everyone: Lessons from an Open Science Collaboration
TypeBlog PostPublishedFeb. 28, 2019, 1:34 p.m....workshops and lessons learned: Since early 2018 the team has grown to include experts who could help provide new... -
Enabling Precision Functional Genomics with the Target Accelerator Plasmid Collection
TypeBlog PostPublishedMay 11, 2017, 2:30 p.m.... complement the one assay per gene approach that has been the historical standard. Specifically, in our... -
Screening for Successful Genome Editing with Digital PCR
TypeBlog PostPublishedMarch 30, 2017, 2:30 p.m....as an emerging validation technology. Digital PCR has several advantages over mismatch nuclease assays ... -
Top Requested Lentivirus and AAV of 2016
TypeBlog PostPublishedJan. 6, 2017, 3:56 p.m....dual-vector system as opposed to a single-vector system) has several benefits. First, the low viral titers typically... -
Choosing a CRISPR Nuclease: Site Accessibility, Specificity, and Sensitivity
TypeBlog PostPublishedNov. 5, 2019, 1:28 p.m....the most widely used CRISPR nuclease, recent work has shown that alternative enzymes may better suit specific...