We narrowed to 893 results for: IND

... new blog post, and live tweet from conferences. Find more tips on using social media as a scientist here...Network – Mingle with pros Where do you meet like-minded science communicators? Attend the annual flagship...started writing three years ago on a field trip in Indonesia. Since then, I have tried many things: photography...Communication Additional Resources on Addgene.org Find Job Openings at Addgene ...

...protein variant lacking its endonuclease ability, to bind to genes without editing the genome (Qi et al., ...complementary to the sequence of interest. Upon binding, CRISPRa systems recruit transcription factors ...VP64 fusion protein engineered with aptamers that bind to MS2 proteins. These MS2 proteins then recruit...Resources on Addene.org Read Addgene's CRISPR Guide Find CRISPR plasmids for your research ...

...during undergrad. Lauren Flynn is the Addgenie behind our "Meet an Addgenie" series. Before heading off...is a more emphasis today about non-academia and industry jobs. Since there are a variety of different career...can make big changes in your career path and still find comfort and happiness there. Read all of the Meet...process, my advisor encouraged me to keep my values in mind as I applied. Thinking about this made me curious...

...poster In almost all scientific posters you will find a title, author names and affiliations, introduction...University. The idea is that the main research finding is written across the center of the poster in a...be cognizant of audience members that are color-blind; avoiding red and green is a good idea for this ... this blog post on navigating the poster session Find tips to make the most of your conference attendance...

...access to some cells and tissues, such as human induced pluripotent stem cells (hiPSCs). Nanoblades, a ...different types of CRISPR cargo including RNPs for indel formation via NHEJ, precise modification with Homology...was not detected and Nanoblade injection did not induce morbidity. Together these results demonstrate that...Addgene.org Check out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral ...

... is a small piece of DNA that can replicate independently from its host’s chromosome. Although we usually...of conjugation, and these are encoded by certain kinds of plasmids — more on that in a minute! Fun fact...antibiotic resistance-mediated selection is indispensable for cloning work, antibiotics and plasmid-encoded...biology, especially when combined with natural inducible expression systems that allow tuning of metabolic...

...given site. While it is possible to "get lucky" and find a great gRNA with a proximal PAM right where you...solution to the PAM problem seems simple, in theory: find a Cas enzyme or evolve one that uses a PAM with ...flexibility could in theory promote off-target binding. The activity of SpRY was validated in double stranded... the genome! Want to get your hands on SpRY? Find a mammalian plasmid here and edit on! References...

...these tools with the Zika virus research community. Find the Zika Sensor Constructs at Addgene As we report...forward, our short-term efforts are focused on finding the resources necessary to advance the technology...Components." Cell (2016). PubMed PMID: 27160350. Find Plasmids from the publication at Addgene 2. Green...Check out All the Plasmids from the Collin's Lab Find Synthetic Biology Plasmids for Your Next Experiment...

...technical challenges they can present. Many researchers find themselves plagued by troubleshooting a virus-based... during transfection, and does not necessarily indicate the production of virus. Alternatively, if the...gene transfer efficiency by enhancing receptor-independent virus adsorption on target cell membranes." Biophysical...Addgene.org Check out Our Viral Vector Guide Pages Find Virus Protocols Order Virus ...

...become increasingly restricted to academic and industrial labs, but recently there has been a counter movement...bioinformatics pipeline. It’s possible that we’ll find something interesting and contribute it back to ...when starting a new project at a makerspace is finding the financial resources to pursue it. Since makerspaces...area, please stop by. And if not, I suspect you can find a makerspace somewhere near you. The place to start...

... way to bring out these interesting stories and remind the panelists of the stories or topics you know...correct date, location and parking information and remind them about the event as it approaches. Room set-up...if slides are allowed. Don't put your panelists behind a table. It adds a formality and distance that ...few questions. Who is out there? What do they do? Find out their areas of science or expertise by listing...

... exciting story, and are ready to present your findings at a conference. But what you get out of a conference...easier through social media. Use social media to find out who’s going to the conference and who you’d ...for any relevant hashtags and handles to help you find other scientists attending. Reach out if you’d like...live-tweeting any sessions that the presenter doesn’t mind sharing on social media. If you could not attend...

...However, they can also be given retro-orbitally, behind the eye. IV injections are used for a broad range...scruffing or mechanical restraint Location: back or hind legs Gauge: 25–27 Volume: < 2–3 ml (~1 ml per site... Back scruffing or mechanical restrainer Back or hind legs; between skin and muscle 25–27 < 2–3 mL (~1...Press. Turner, P. V., Brabb, T., Pekow, C., & Vasbinder, M. A. (2011). Administration of substances to...

... Off Resources on Addgene.org Find Mammalian Expression Vectors Find Cre-Lox Plasmids Learn More about...with a gRNA). Be sure to keep these techniques in mind when choosing a knockout strategy! Knockout plasmids...knocked out. Use positive and negative selection to find correctly recombined cells. Recombination is a rare...

...they are transferrable across most, if not all, industries and work environments. This is why they are called...co-workers want to know that you are a well-rounded individual with more going for you than your technical expertise...develop your nascent leadership skills. You might just find that you’re more of a leader than you thought! When...into pretty much any other workplace. To get your mind working, take a look at the short list of transferrable...

...located in. Otherwise it could take us hours just to find one sample! Luckily, if a rack of tubes is dropped... barcode also identifies the contents of each individual tube.  If a plate of samples is ever dropped,...know what is in each one. Even if we lose a tube behind a freezer or under a lab bench, we will know that...container a specific location to know exactly where to find your samples. Additionally, giving each sample a...

...37C Y66W Causes the chromophore to form with an indole rather than phenol component (cyan derivatives)...one fused to each protein or domain being tested. Find FRET plasmids here. Split EGFP: An alternative to... more about optogenetics at OpenOptogenetics and find optogeneic actuators and sensors at Addgene. Cell... Gal will share his list of 10 things to keep in mind when choosing the best fluorescent protein to use...

...the years we have accumulated more than 300,000 individual nucleotide sequences. More than half of these...Quick-stepping to a FASTA file   With our outline of individual steps to automate plasmid sequence assembly, ... of one-off scripts or programs, our goal was to find a suitable software tool that could orchestrate ... More resources on the Addgene blog:  The Power Behind NGS Plasmid Validation: SeqwellHow to Keep a Lab...

...immunology or subpopulations of cells, you’ll soon find yourself encountering flow data in the literature... will have numbers by gated populations; this indicates the percentage of total events contained in the... gated population. Heat maps are often used to indicate the relative density of cells. You can see both...presented. A horizontal line across the peak will indicate the cut-off value for a positive signal (Figure...

... also depends on the individual library. So, on each library’s page, you can find protocols for sequencing...amplification. And as anyone who has worked with individual plasmids knows, some plasmids amplify well and...troubleshooting blog should you run into any issues.  Keep in mind that a low MOI will increase the chances of a cell...