We narrowed to 784 results for: IRE

...one since the formation of mEosFP’s chromophore requires a temperature below 30°C and this is not ideal...fluorescence localization data. However, this technique requires harsh fixation conditions to preserve cellular...

...cell that gets the plasmid contains all of the desired gRNAs. This increases the chance that all the edits...editing, and a subsequent curing protocol that requires only 2-3 hours incubation. Kondo Lab multiplexed...

... you to measure the intensity of signal over an entire cycle. Since signals are produced by the presence... practice, multiplexing is quite difficult and requires significant optimization to ensure the results...

...helped us manage our data. To quote Jason Niehaus, Director of Data Science and head of our Data Team, “Our...

...December are eligible to win a: That will warm up the entire lab (hint: you might need some mini marshmallows...

...win a hot chocolate party that will warm up the entire lab. We’ll choose 4 winning submissions and deliver...

...describe newly engineered Cas9s with more flexible requirements and enhanced editing, as well as alternatives...

... or on our hot plasmids webpage. Listen to the entire Hot Plasmids podcast! Hot plasmids covered in this...

...genome insertion, without DNA cleavage, with CRISPR-directed integrases🎵 On the 6th day of CRISPR, Addgene...

...creating memories with their labmates! Are you inspired by our 2017 #Deckthelab winners? Be sure to enter...

...Cultured Warrior (made by Nate using pipette boxes, wire, cable ties) received a festive makeover today✂️...

...Jillian Parker who published their work on a protein required for cytokinesis in JEI. Developing an interested...

...faster, as no license, or license fee, will be required. If you have any questions on ordering plasmids...

...tackle any experimental questions you may have. Our Director of Content, Brook, and our scientific curation...

...for materials distributed by Addgene.  Future directions As the AAV Data Hub grows, we hope to mine the...

...ancestral bias in CRISPR guide library design.  dCas9-directed DNA Demethylation: Guest blogger Daniel M. Sapozhnikov...

...biosafety cabinet ventilation, and lab lighting/HVAC requires a significant energy expenditure here at Addgene...

...using this PEI Calculator. AAV packaging typically requires transfecting three plasmids at specific molar ...

...from one of our recent newsletters. Listen to the entire Hot Plasmids episode! We generally publish 5-10...

...plasmids. Simply click on the badge to be taken directly to the plasmids on Addgene’s site (Side note: ...