We narrowed to 676 results for: Mos;
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TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....common lab cloning strain has failed so far. What is most important is the transformation efficiency of the...
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New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog PostPublishedOct. 4, 2018, 12:44 p.m....overexpression library identified several overlapping genes, most genes were unique to the individual libraries. These... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....work in the future; they are transferrable across most, if not all, industries and work environments. This... -
AAVs CREATed for Gene Delivery to the CNS and PNS
TypeBlog PostPublishedSept. 28, 2017, 2:01 p.m.... Adeno-associated viral (AAV) vectors are the most frequently used gene-transfer tools in the study... -
MXS Chaining
TypeBlog PostPublishedFeb. 7, 2017, 3:30 p.m....because CpG dinucleotides are underrepresented in most vertebrate genomes (Josse et al., 1961, Swartz et... -
Course-Based Undergraduate Research Experiences (CUREs)
TypeBlog PostPublishedFeb. 9, 2023, 2:15 p.m....), decided to take a different approach. His own most impactful learning experiences happened in the lab... -
CRISPR 101: Making a Knock-In Cell Line
TypeBlog PostPublishedDec. 22, 2022, 2:15 p.m.... other edits Like I said earlier, HDR is not the most common editing pathway for DNA repair. To tilt the... -
Isolating Genomic Regions of Interest with the CRISPR System
TypeBlog PostPublishedMay 30, 2019, 12:44 p.m....numerous genomic loci through proteomics and the chromosome conformation capture (3C) technique. In addition... -
INTEGRATE: Bacterial Genome Engineering Using CRISPR-Transposons
TypeBlog PostPublishedDec. 7, 2020, 2:53 p.m.... R, Jiang Y, Yang J, Yang S (2020) Multicopy Chromosomal Integration Using CRISPR-Associated Transposases... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....42% editing efficiency. They note that RESCUE is most active in a 30 nt guide when C or U base-flips are... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....available to evaluate AAV vector performance. Yet, most of the technical data needed to replicate and build... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....cellular and tissue morphology. Formaldehyde is the most commonly used cross-linker for IF. However, you ... -
Droplet Digital PCR for AAV Quantitation
TypeBlog PostPublishedJan. 16, 2020, 2:18 p.m....virus in reaction mix D = Dilution factor of virus Most droplet readers have a few channels for detecting... -
Tips for arabidopsis transformation
TypeBlog PostPublishedOct. 25, 2018, 1:23 p.m.... region, it may cause unexpected phenotypes. For most phenotypic analysis it is appropriate to begin working... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m....Designing colony PCR primers The first and perhaps most important step to colony PCR is designing primers... -
Tips for Screening with Yeast Two Hybrid Systems
TypeBlog PostPublishedOct. 22, 2015, 2:30 p.m....will increase the stringency of your binding. And most importantly: Independently validate identified binding... -
Cpf1: A New Tool for CRISPR Genome Editing
TypeBlog PostPublishedOct. 14, 2015, 2:30 p.m....does not mediate detectable off-target cleavage. Most gRNAs directed low-frequency Cpf1 cleavage at 1-... -
Tips from the Repository Trenches: Using Barcodes to Track Samples
TypeBlog PostPublishedOct. 6, 2015, 2:30 p.m.... different cells than were originally requested. Most of our samples (98.2%) transform the first time ... -
A Career in Grants: How To Become a Grant Professional
TypeBlog PostPublishedAug. 20, 2015, 2:30 p.m....career path. A part of the career path story for most established grant professionals is learning to succeed... -
Analyzing CRISPR Editing Results with ICE from Synthego
TypeBlog PostPublishedMay 8, 2018, 1 p.m....multiplex sample, the cut site will be aligned to the most 5’ cut site. Indel Distribution In the Indel...