We narrowed to 868 results for: REN;

...for either a circular or linear assembly. If you aren’t constricted to a specific number of fragments, ...base or 4-base overhangs, and with a variety of different enzymes and cycling conditions (see Table 1). ... and don’t forget to ask DAD for some help! References Potapov, V., Ong, J. L., Kucera, R. B., Langhorst...

...transactivator, Tat. For more information on the different generations of lentiviral packaging systems please...experiment, it is recommended that you try a few different ratios of transfer, envelope, and packaging plasmids... Addgene’s website for additional resources. References Kumar M, Keller B, Makalou N, Sutton RE. Systematic...

...a blot, you should not compare the strength of bands from different rounds of detection. Finally, stripping...abundant. If the abundance of your targets isn’t too different, consider antibody affinity: stronger binding ...

...publish a new blog post, and live tweet from conferences. Find more tips on using social media as a scientist...communicate science. You can draw live sketches at conferences like @ATJCagan or create agar art with colorful...active with it, new doors started opening up. My current job as a Marie Curie Fellow is a direct consequence...

...immunogenicity and range of serotypes allowing preferential infection of certain tissues. However, these...deletions at the 3’ end of the target sequence. Cpf1’s strength may lie in its low off-target editing rates, determined...Cpf1 fits into the genome editing landscape. References 1. Kim D, Kim J, Hur JK, Been KW, Yoon SH, Kim...

...white arrowheads). It is necessary to use two different pairs of target sites for this strategy to work...another. In the field of mouse genetics, FLEx is currently being used to study a wide variety of in vivo ...the genOway press release for more information. References: 1. Schnütgen F, Doerflinger N, Calléja C, Wendling...

...workshops, presentations, and lectures. There are currently around 1,000 active makerspaces around the world...learning and communicating with one another.      Currently our main focus is coding the molecular biology...academic labs, we have the capability to study many different mutants because the Caenorhabditis Genetics Center...

...format for events and sessions in scientific conferences. They can result in lively discussions with both...the moderator is not a panelist and will play a different role so her ability to control the event is what...spontaneity so a moderator should be prepared with different questions than those on the prep calls, but often...

...image provided by Manuel Gonçalves; made with BioRender.   Delivering gRNA and Cas9 using adenoviral...and CRISPR resources on Addgene's CRISPR page. References Beatty MS, Curiel DT (2012) Adenovirus Strategies...Zamyatnin AA Jr (2016) Viral vectors for gene therapy: Current state and clinical perspectives. Biochemistry Moscow...

... the heat. Isolating male and female flies Differentiating male and female flies is essential for setting...their adult phase. It is important to clear out the parent crosses from the container before the progeny begin...thorax to look like stubble (sb). For a better reference of various fly phenotypes, check out the Classical...

...these vectors is controlled by a different promoter resulting in different levels of expression from each...labelled or modified amino acids that are useful in different downstream applications. Cell-free expression ...

...AstraZeneca. Why I chose an industry postdoc I’m currently doing an industry postdoc in neuroscience at AstraZeneca... the process is similar to academia. The only difference is that your paper will be reviewed by an internal...blogger, Laura Desrochers! Laura Desrochers is currently a Postdoctoral Scientist at AstraZeneca. She has...

...most cases, by using monomeric FPs or FPs from different families, which cannot form heterodimers (i.e....at the National Institutes of Health and he is currently an Associate Professor at Albert Einstein College...avid long distance runner, gardner, and cook.   References 1. Snapp, E.L., et al., Formation of stacked ...

...outcomes can be most effectively achieved with different CRISPR components. Select the appropriate CRISPR...cell lines is quite broad and includes several different methods, so we've summarized some key features...vitro cell culture. The user should review the current literature about their preferred model.  If your...

...expression of Cas9/gRNA and can be used for most of the current genome engineering applications of CRISPR including...comes down to the researcher’s familiarity with different systems.  Using Cas9-gRNA RNPs in your experiments...include genome engineering and microbiology.     References 1. Chen, Sean, et al. "Highly efficient mouse...

...Purification Good for antibody-based purification; has inherent enterokinase cleavage site GST Large Protein...protein as pure as possible? Vectors that offer different combinations of tags are readily available, and...information and some protocols can be found in the references provided. Additional resources: Young CL, Britton...

... has been crucial for us to keep pace with our current need to analyze nearly 300 sequences each week.... address the need to accelerate science using different approaches. We were also encouraged to see that...pipelines for genomic data. Thanks to the flexibility inherent in Nextflow’s design, we were free to use multiple...

... the Howard Hughes Medical Institute. Are you currently on or planning to go on the academic job market...if your model is correct or your data favor a different model. Finding evidence for or against a model...the answer to your research problem. Creating a coherent unified story and model can help make your ideas...

...jarring for the unprepared, and unfortunately, there aren’t a lot of resources out there for scientists making...tight-knit team like that can and will be a little gut-wrenching, but you can get that feeling of camaraderie again...etc.--so everyone’s experience will be a little different. Some of these changes you may enjoy immediately...

...writer? That’s okay! Your cover letter needs to be coherent and free of typos and grammatical errors. It does...skills and experiences fit the job requirements, strengthened an application. Even so, there were examples...experience, having given more than 20 talks at conferences and taught for five years at [name of the university...