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Showing: 621 - 640 of 835 results

  1. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...neutralize the nuclease activity. For strains that still carry this gene, there are usually extra steps like...the pH preparation stabilizing the plasmid, and still allowing most downstream processing. On the other...

  4. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ... my kids like any other youngster. His parents, Kristi and Rico, had to keep him from running too much...the fight against FOP. Second, Addgene will be hosting the AJ’s Boston League team (Contributing many ...

  6. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...plasmid in addition to its own chromosomal DNA, costing additional resources to maintain the plasmid. Adding...cell division (bacteriostatic); however, the distinction between the two categories may be a bit of a ...

  7. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...because the secondary antibody is not able to distinguish between the primary antibody and endogenous IgGs...your samples, but know that all is not lost if you still see non-specific staining! Hopefully, these solutions...

  8. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ... viral vectors, which are used in the lab, are distinct from viruses — we’ll get to that in a bit! Viral...bud out, instead of lysing, but they do generally still kill the infected cell.) This is in contrast to ...

  9. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...the fetal tissue which HEKs were isolated from is still a mystery, but it’s currently assumed that the cells...native channels.  No matter what your research question, HEK-293 is known for reproducible results. It...

  11. CRISPR Challenges: Standardization and Homology Directed Repair

    Type
    Blog Post
    Published
    Feb. 14, 2018, 2:36 p.m.
    ... show that this basic concept of gRNA design is still challenging for researchers using CRISPR, and that...you’d like to avoid editing the genome and just stick to RNA? Cas13 may be able to help you do that! How...

  12. In Vivo Biotinylation of Bacterial Fusion Proteins

    Type
    Blog Post
    Published
    Jan. 25, 2018, 2:09 p.m.
    ...Backstrom, a biochemist in the Vanderbilt Eye Institute and Tonia Rex's lab. A common strategy to determine...Backstrom, Ph.D is a biochemist in the Vanderbilt Eye Institute and is particularly interested in cell signaling...

  13. Identifying Sequence Elements with SnapGene's Feature Database

    Type
    Blog Post
    Published
    Dec. 21, 2017, 2:06 p.m.
    ...But what makes software good? Fortunately, that question has been thoroughly answered by experts in human-computer...engaging with users of the software. We welcome suggestions about common features from anyone who uses SnapGene...

  19. Safe Port in a Storm...How Addgene is Weathering the Pandemic

    Type
    Blog Post
    Published
    Sept. 17, 2020, 1:15 p.m.
    ...world mobilized on COVID-19 research, cures, and diagnostics. Almost immediately we began to receive urgent...Addgene plays in accelerating research. Yet this was still not enough to ensure we could keep up with demand...

  20. Fluorescent Biosensors for Measuring Autophagic Flux

    Type
    Blog Post
    Published
    Jan. 22, 2019, 2:41 p.m.
    ...of red to green fluorescence allows for clear distinction between vacuolar/lysosomal and cytoplasmic Rosella...acting as an internal control. Autophagic flux is estimated by the GFP/RFP ratio: a decrease in GFP fluorescence...
Showing: 621 - 640 of 835 results