We narrowed to 642 results for: Vars;

1. New Acoustic Reporter Genes: Ultrasound Imaging of Gene Expression

   Type

   Blog Post

   Published

   Jan. 24, 2023, 2:15 p.m.

   ...ended up being very straightforward to engineer various cell lines from different tissues and mammalian...

2. Bioinformatics at Addgene

   Type

   Blog Post

   Published

   July 14, 2022, 1:15 p.m.

   ...re-use or borrow existing code and control how the various pieces were connected, as this enabled us to rapidly...

3. New CRISPR Tools: Cas7-11 and PASTE

   Type

   Blog Post

   Published

   Feb. 17, 2022, 2 p.m.

   ...then used this information to identify a large variety of related bacteria with similar Cas systems. Some...

4. Viral Vectors 101: Pseudotyping

   Type

   Blog Post

   Published

   April 20, 2021, 2:30 p.m.

   ...cytotoxicity and the mechanisms behind the cytotoxicity can vary. For example, the popular VSV-G envelope protein...

5. Plasmids 101: Plasmid Incompatibility

   Type

   Blog Post

   Published

   March 31, 2020, 1:15 p.m.

   ...Reference References  del Solar G, Giraldo R, Ruiz-Echevarría MJ, Espinosa M, Díaz-Orejas R (1998) Replication...

6. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

   Type

   Blog Post

   Published

   Feb. 18, 2020, 2:15 p.m.

   ...the Mentor-Singleton relationship. Some of the variability was organic, as some people need less assistance...

7. Important Considerations in Optogenetics Behavioral Experiments

   Type

   Blog Post

   Published

   Oct. 1, 2015, 2:30 p.m.

   ...brain region of interest could be low or highly variable without the behavioral test. Another readout may...

8. Plasmids 101: Biotinylation

   Type

   Blog Post

   Published

   Nov. 15, 2018, 1:50 p.m.

   ...chemicals (biotinylation reagents). There are a variety of biotinylation reagents available that differ...

9. March for Science

   Type

   Blog Post

   Published

   April 21, 2017, 2:30 p.m.

   ...her PhD from the Systems Biology department at Harvard University. She is a scientist with a passion for...

10. Using Phosphoserine to Study Protein Phosphorylation

    Type

    Blog Post

    Published

    June 23, 2016, 2:30 p.m.

    ... temperature and length of induction, which will vary between different proteins. Beyond these standard...

11. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

    Type

    Blog Post

    Published

    Sept. 5, 2017, 1:58 p.m.

    ...the Addgene Blog Learn about High Fidelity Cas9 Variants How to Design Your gRNA Delivering CRISPR Ribonucleoproteins...

12. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type

    Blog Post

    Published

    July 10, 2018, 1:08 p.m.

    ... avoid scattering intellectual property across various software providers used to supplement their paper...

13. CRISPR 101: Validating Your Genome Edit

    Type

    Blog Post

    Published

    Nov. 3, 2022, 12:15 p.m.

    ...Pinello L. CRISPRitz: rapid, high-throughput and variant-aware in silico off-target site identification ...

14. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    Published

    Jan. 4, 2022, 2:15 p.m.

    ...basic approaches to conflict resolution based on various levels of assertiveness and cooperation: avoiding...

15. Antibodies 101: Introduction to Immunofluorescence

    Type

    Blog Post

    Published

    Nov. 16, 2021, 2:15 p.m.

    ...produce and distribute antibodies validate them in a variety of applications, including IF (or IHC or ICC), ...

16. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type

    Blog Post

    Published

    Jan. 26, 2016, 3:30 p.m.

    ...diseases, the mutational landscape is broad and varied, necessitating the development of many distinct...

17. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    Published

    July 12, 2016, 2:30 p.m.

    ...equal. Some work better than others. There are a variety of resources online to guide you in gRNA design...

18. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type

    Blog Post

    Published

    Dec. 17, 2020, 1:30 p.m.

    ...being used in any genetic background, but require variable amounts of experimental manipulation and screening...

19. Antibodies 101: Designing Your First Flow Panel

    Type

    Blog Post

    Published

    May 28, 2024, 1:45 p.m.

    ...Research Associate in the lab of Arlene Sharpe at Harvard Medical School. His projects include investigating...

20. Special Delivery: Fluorophore Targeting for FRET Studies

    Type

    Blog Post

    Published

    July 19, 2016, 2:30 p.m.

    ...genetically-encoded FRET donors (Fig. 1). Scores of FP variants have been created, thereby enabling a broad spectrum...