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Showing: 621 - 640 of 911 results

  3. Gaining Leadership Skills Volunteering at a Professional Organization

    Type
    Blog Post
    Published
    Aug. 6, 2015, 2:30 p.m.
    ...acquire during their PhD and postdoctoral training. Addgene executive director Joanne Kamens has written a ...Further Reading Find more Career articles on the Addgene Blog Read Joanne Kamen's Management for Scientists...a 5-part blog on the topic:  http://blog.addgene.org/management-for-scientists-managing-vs-leading. The...

  5. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...Kamens on BitsizeBio  Additional Resources on the Addgene Blog Read our "Management for Scientists" series... on What Makes a Good Mentor Resources on the Addgene Wesbite Find plasmids to Expedite Your Experiments...

  6. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...Antibodies Resources on Addgene.org Addgene's Western Blot Protocol Addgene's Western Blot Protocol Video...Looking for a western blot protocol? Check out Addgene's western blot protocol and protocol video!  Antibodies...Resources and references More resources on the Addgene blog Antibodies 101: The Basics of Western BlottingAntibodies... Good luck and happy blotting! Many thanks to Addgenies Ashley Waldron, Meghan Rego, and Amrita Rhodes...

  7. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ...fluorescent microscopy technique for your study see Addgene’s blog post Which Fluorescence Microscopy Techniques...experiment and are unsure which to choose, check out Addgene’s article: Which Fluorescent Protein Should I Use.../s10616-011-9415-0 Additional resources on the Addgene blog Check out other methods using fluorescent...proteins Learn more about antibodies Resources on Addgene.org Visit the Fluorescent Protein Guide Find antibody...

  8. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

    Type
    Blog Post
    Published
    Dec. 17, 2020, 1:30 p.m.
    ...perform pha-1 co-conversion are available through Addgene. In 2019, Farboud et al. described another similar...To see more CRISPR tools for C. elegans, visit Addgene’s C. elegans CRISPR plasmids page. Future perspectives...about genome editing with CRISPRs? Check-out Addgene's CRISPR Resources Learn about gRNA design and...

  9. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...reduce your lab’s environmental impact, check out Addgene’s blog post on bringing sustainable practices for...relatively little work after the initial setup. (Addgene actually uses some of these — if you’re curious...Nyla Naim. Resources Additional resources on the Addgene blog Plasmid Preps: Different Purity, Different...: Easy Action Items Additional resources on addgene.org DNA Quantification Protocol Recovering Plasmid...

  10. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...Resources on Addgene.org Addgene's Antibody Guide Ready-to-use antibodies in the Addgene repository Antibody...sailing, and reading.   More resources on the Addgene blog Antibodies 101: Introduction to Gating in ...

  11. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    Published
    July 1, 2022, 1:15 p.m.
    ...                Two of the platforms we use at Addgene: YouTube, for longer educational videos, and Twitter...Internet "trolls"   More resources on the Addgene blog:  Summer SciComm Series: Cognitive LoadIntro...

  12. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ...concentration of the original template DNA in the sample.  Addgene currently uses ddPCR for AAV titrating. You can...Elsevier, pp 215–247 Additional resources on the Addgene blog Droplet Digital PCR for AAV Quantification...Site Directed Mutagenesis by PCR Resources on Addgene.org Protocol: Polymerase Chain Reaction (PCR) Protocol...

  13. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    Published
    April 4, 2019, 12:53 p.m.
    ...bacteria of interest? Find Mobile-CRISPRi plasmids at Addgene! References Auchtung, Jennifer M., et al. "Biology...PubMed PMID: 26104363. Additional resources on the Addgene blog Learn about other CRISPR methods for bacterial...libraries Read all CRISPR blog posts Resources on Addgene.org Browse our CRISPR interference plasmids Find...

  14. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

    Type
    Blog Post
    Published
    Feb. 7, 2019, 2:16 p.m.
    ...at existing tools. As has been written here on Addgene, there has been a Cambrian-like explosion of software... Clement Andy May Additional resources on the Addgene blog Read our CRISPR 101 blog posts Learn about...gRNAs Find other CRISPR software Resources on Addgene.org Find CRISPR plasmids and resources Download ...

  16. CRISPR 101: Ribonucleoprotein (RNP) Delivery

    Type
    Blog Post
    Published
    Sept. 6, 2018, 12:02 p.m.
    ...advancement. Andrew Hempstead is a Senior Scientist at Addgene.  Andrew's interests include genome engineering...PMCID: PMC5681539. Additional resources on the Addgene Blog Subscribe to CRISPR blog posts Learn about...techniques Find tips on gRNA design Resources on Addgene.org Browse all CRISPR plasmids Find validated gRNAs...

  17. 10 Basic tips for mammalian cell culture

    Type
    Blog Post
    Published
    July 12, 2018, 1:09 p.m.
    ...many times and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage...Second  Edition), 2012. Additional Resources on the Addgene Blog The Challenges of Cell Culture Cloning Mammalian...Plasmids 101: Mammalian Vectors Resources on Addgene.org Find mammalian plasmids for your research Find...

  18. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ...recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide webpage, and references 1, 2....PMCID: PMC5609467. Additional Resources on the Addgene Blog Using virus in your research - a primer for...troubleshooting viral transductions Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page...

  19. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...this paper are available at Addgene.  Additional Resources on the Addgene Blog Learn about Engineered...about Homology Directed Repair Resources on Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids...

  20. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type
    Blog Post
    Published
    June 22, 2017, 2:30 p.m.
    ...constructs you create in your own work available to the Addgene community. Download our Markers of Subcellular ... FRET Microscopy. Additional Resources on the Addgene Blog Learn More about Plasmids for Endogenous ...Collection Get Tips for Using FRET Resources on Addgene.org Browse the Allen Institute for Cell Science ...
Showing: 621 - 640 of 911 results