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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 803 results for: cha;

Showing: 621 - 640 of 803 results

  1. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    Published
    Sept. 3, 2015, 2:30 p.m.
    ... included a simple corn hole set and a cooler. Chances are one of your colleagues has a frisbee at least...where to be and when. This pre-planning will avoid chaos and delays in getting the fun started. I’m aware...

  2. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...lentiviral and retroviral packaging vectors are not interchangeable. While these differences may seem subtle they...the generation used to package the virus does not change the generation of the transfer vector. Q5: What...

  3. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type
    Blog Post
    Published
    July 8, 2025, 1:15 p.m.
    ...type of parts to ensure that these would be interchangeable between different laboratories. Therefore, ... a restriction enzyme recognition site without changing the function of the sequence, normally through...

  4. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...My Bio 101 professor displayed a codon table — a chart every molecular biologist has seen before, showing...bacteria and eukaryotes. But be careful — antibiotic mechanisms vary! Aminoglycoside antibiotics also block protein...

  5. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...during cell division. They don’t simply rely on chance (or random diffusion) to make this happen.  There...centromere-based incompatibilities: Insights into the mechanism of incompatibility mediated by low-copy number...

  6. Plasmids 101: Gateway Cloning

    Type
    Blog Post
    Published
    Jan. 12, 2017, 3:30 p.m.
    ...destination vectors prior to recombination, and it is exchanged with the gene of interest during the BP or LR ...Multiple Hosts. Curr Protoc Protein Sci. 2003 Feb;Chapter 5:Unit 5.17. PubMed PMID:18429245. 3. Ptashne, ...

  7. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...Although different types of cancer have different challenges and obstacles to overcome, mesothelioma sufferers...of immunotherapy is the use of drugs (or other mechanisms) that work through body’s immune system to fight...

  8. Viral Vectors 101: Pseudotyping

    Type
    Blog Post
    Published
    April 20, 2021, 2:30 p.m.
    ...have different levels of cytotoxicity and the mechanisms behind the cytotoxicity can vary. For example...differentially inactivated by nonspecific complement mechanisms in serum. Sometimes this is due to the cells ...

  9. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ... been converted to an alanine (D10A or H840A), changing its double-strand cut to a single-stranded nick...Walton et al., 2020). Further, this group also characterized a second Cas9 variant, SpRY, which cuts at NRN...

  10. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    Published
    Nov. 19, 2020, 2:15 p.m.
    ... Rett Syndrome, respectively. One of the main challenges facing Rett researchers is how to deliver the... by Gail Mandel. There are numerous technical challenges to overcome for both of these approaches, such...

  11. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    Published
    Feb. 10, 2015, 3:06 p.m.
    ...suited for recombinant protein expression. Many challenges can arise when over-expressing a foreign protein...minutes before adding the inducer. Growth Media: Changing media is tricky, because there can be a trade-...

  12. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...might suggest, an RNA-mediated gene silencing mechanism. RNAi was a landmark discovery in the 1990s and..., C. (2018). Overview of MicroRNA biogenesis, mechanisms of actions, and circulation. Frontiers in Endocrinology...

  13. Antibodies 101: Introduction to Gating in Flow Cytometry

    Type
    Blog Post
    Published
    April 4, 2024, 1:15 p.m.
    ...ll notice that while the height of your events changes, the separation pattern of positive vs. negative...live population from my live-dead staining. (B) Changing the y-axis from SSC to FSC can alter the positioning...

  14. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...enzyme screening is sensitive to single base pair changes, but the edit must occur at an existing restriction...simultaneously validate edits and look for off-target changes. This method requires a set of control cells to...

  15. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ... This test can be performed in two steps for challenging samples as in Two-Step-SHERLOCK or in a fast,...the immobilised target sequences. Upon binding, changes in fluorescence can be visualised. Another approach...

  17. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...role of renaturation temperature in the polymerase chain reaction." Analytical Biochemistry 209.2 (1993):...Xia, Yongzhen, et al. "New insights into the QuikChangeTM process guide the use of Phusion DNA polymerase...

  18. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

    Type
    Blog Post
    Published
    May 10, 2016, 2:30 p.m.
    ... a well-established viral reservoir. A second challenge for CRISPR HIV-1 therapies is sequence specificity.... Ramanan V, Shlomai A, Cox DB, Schwartz RE, Michailidis E, Bhatta A, Scott DA, Zhang F, Rice CM, Bhatia...

  20. Plasmids 101: Common Lab E. coli Strains

    Type
    Blog Post
    Published
    Nov. 7, 2014, 2:56 p.m.
    ...below outlines a few of the more common genetic changes found in E. coli strains. Table 1: Common gene ...are methylation sensitive. dnaJ Mutation in a chaperonin gene Increases the stability of certain expressed...
Showing: 621 - 640 of 803 results