We narrowed to 846 results for: ins

...AAV does not integrate into the host genome. AAV instead relies mainly on episomal expression. Episomes ... Even more creative options involve generating strains of mice that stably express Cas effectors in specific...doi.org/10.1038/s41596-022-00700-y Hanlon, K. S., Kleinstiver, B. P., Garcia, S. P., Zaborowski, M. P., Volak...

...precise modifications of DNA (replacements, small insertions, and deletions) without introducing DNA double-strand...screens such as PRIDICT/PRIDICT2.0 provide valuable insights into pegRNA editing efficiencies for specific ...Parts, L. (2023). Prediction of prime editing insertion efficiencies using sequence features and DNA repair...

...price point, which should be carefully balanced against the limitations of the assay.      Fig....immersion technique, courtesy of CAR15.) Each droplet contains a primer and probe set for both the gene of interest...only ddPCR provides absolute quantitative data, instead of relative quantification.  Even though no one...

...cochlea in 3D. The basic configuration of the instrument used in this reconstruction lay the foundation...cleared whole-mount specimens, such as whole mouse brains expressing green fluorescent protein (GFP) (6)....solvent cleared organs) method of imaging rodent brains and spinal cords by combining Ultramicroscopy with...

...concentration of a serially diluted standard is plotted against its OD560 measurements and the trendline y = 0.6561x...the minimum and maximum detection limits of the instrument being used to measure the response. The dynamic... to set yourself up for success!  Study the instruments and specific assays you will be using ahead of...

... Cas13 enzymes do not contain the RuvC and HNH domains responsible for DNA cleavage, so they cannot directly...functional A->G change. They fused ADAR deaminase domains (ADARDD) to dPspCas13b, but observed low RNA editing...perform C-to-U edits, the authors note that it retains its adenine deaminase ability. The authors then...

...using water-oil emulsion technology. Each droplet contains the ingredients for amplification of the target...experiment. The process to titrate AAV by ddPCR begins with diluting the virus. It is important to note...containing approximately 20,000 droplets. Each droplet contains the material required for a mini amplification...

...be better suited for the job. As single domain proteins, they can be expressed in bacteria, making nanobodies...primary antibodies from different IgG subclasses. Instead, you can pre-incubate individual primary antibodies...Learn more about protein tags Read about E. coli strains for protein expression Additional Resources on...

...Our Parent Teacher Association (PTA) has been instrumental in supplementing that curriculum. It’s through...little intimidating. Too simplistic and I would insult the students. Too complicated and I would quickly... Eric J. Perkins...

...is introduced into the cap plasmid library by inserting short DNA sequences that contain all possible ...to 2-fold higher GFP intensity compared to the strains they were derived from. They also transduced a ...A cocktail of three AAV vectors expressing RGB proteins under the control of an inducer, i.e. tet-off ...

...that couldn’t package itself due to a few missing proteins. The envelope plasmid contained a viral envelope...lentiviral vector containing an antisense sequence against the HIV-1 envelope. As of 2013, 65 patients had...Lentiviral vectors in cancer immunotherapy. Oldham RA, Berinstein EM, Medin JA. Immunotherapy. 2015;7(3):271-84...

...credential from the Grant Professional Certification Institute (GPCI). GPCI evaluates a candidate's expertise...through the Grant Professionals Certification Institute, an Approved Trainer by the Grant Professionals... Social Media Strategist through the National Institute of Social Media. Follow Jo on Twitter @JM_Grants...

... have since adapted it for a host of different proteins and small molecules from a variety of sample types...antibodies and thus are not suitable for low abundance proteins. Because the method uses a single antibody, it... and a series of wash steps removes non-target proteins and molecules from the assay. The user then adds...

...well-supported, so we could focus on our development instead of building the entire ecosystem Proven performance...throughput necessary to keep up with our sequencing instrument (hundreds of plasmids each week!), we already...process. Similar to our first attempt, we manually inspected the output with a print statement at the end of...

...Members of the bacterial world produce an assortment toxins to claim territory or kill competing microorganisms...an efficient cloning tool. The plasmid pKSCTMF contains the mazF gene and a gene for Zeocin resistance... James Kaper used the hok/sok system to create strains that can stably express antigens in sufficient ...

...love you back A successful lentiviral infection begins with your cells, both your packaging cells and ...However, viruses pseudotyped with other envelope proteins may have a limited number of targets and therefore...Finkelshtein, D., Werman, A., Novick, D., Barak, S., Rubinstein, M. “LDL receptor and its family members serve...

...for many labs. However, RNA interference (RNAi) remains steadfast in the background, a handy “oldie” as...cleaves the target mRNA or recruits other silencing proteins. Figure 1: Comparison of shRNA and miRNA...RNAi has retreated a bit to the background, it remains a solid option for altering the expression of specific...

... each project and how useful each item is. For instance, if I don’t have draft blog post ready for publication...prevent you from making progress on a task. For instance, even if I had planned my day extensively, my ...that highlights the things you like about it. For instance, while I may have found splitting cells boring...

...his or her chosen career path,” but individual institutions can have many different names for the positions...From Panel Session 2: Defining the Postdoc: An Institutional Perspective. This lack of complete information...Require that postdoc salaries be paid on fellowships instead of grants such that PIs no longer save grant money...

... sometimes vary between target species or even strains. Pro tip! Getting a vector into a particular cell...growth factor receptor 1 (FGFR1)3 αVβ5 and α5β1 integrins3 Hepatocyte growth factor receptor (HGFR)3 Laminin...tropism varies both between species and even between strains of one species, so be sure to check the literature...