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We narrowed to 816 results for: lars;

Showing: 621 - 640 of 816 results

  1. Some Like it Hot: Thermostable GeoCas9

    Type
    Blog Post
    Published
    Sept. 14, 2017, 12:40 p.m.
    ...overlapping PAM. In both cases, GeoCas9 and SpyCas had similar levels of editing. See figure 4 in Harrington et...thermostability could also be used with in vitro molecular bio applications that require high temperature...

  2. CRISPR 101: Cas9 Nickase Design and Homology Directed Repair

    Type
    Blog Post
    Published
    March 15, 2018, 12:59 p.m.
    ... small deletions, while H840A is biased towards large insertions. These profiles are likely due to the... Oligonucleotides) Use 100 nt homology arms for large insertions (long ssDNA; IDT Megamer® ssDNA Fragments...

  3. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    Published
    July 27, 2021, 1:15 p.m.
    ...internal control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...

  4. Viral Vectors 101: Parts of the AAV Packaging Plasmid

    Type
    Blog Post
    Published
    Dec. 3, 2020, 2:15 p.m.
    ... or p19 promoters to, respectively, produce the large (Rep78 and Rep68) and small (Rep52 and Rep40) Rep...Targeting and Improving the Efficacy of AAV Vectors. Molecular Therapy - Methods & Clinical Development 12:248...

  5. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    Published
    June 4, 2020, 1:22 p.m.
    ...essential cofactors for many tools used in modern molecular biology like restriction enzymes and polymerases...posts Resources on Addgene.org Check out the Molecular Biology Reference Find protocols on plasmid cloning...

  7. FastCloning: A Newer, Simpler Cloning Technique

    Type
    Blog Post
    Published
    Nov. 1, 2016, 2:30 p.m.
    ...Protein Science at the University of Connecticut. Molecular cloning is the isolation, insertion and amplification... recombinant DNA without sequence alteration. Molecular cloning techniques are some of the most widely...

  8. Running for Rare Disease, Running for FOP, Running for AJ

    Type
    Blog Post
    Published
    Sept. 15, 2016, 2:30 p.m.
    ... not seem much different than my second son of similar age. When he came to visit us and watch fellow ...jarring of his body could initiate very painful FOP flare ups. Eventually, without a cure, AJ will begin losing...

  9. Management for Scientists: Giving Feedback

    Type
    Blog Post
    Published
    Feb. 6, 2015, 2:46 p.m.
    ...style. Formalizing the feedback session Giving regular, informal feedback is a very effective way to bring...employee. This is especially true if the problems are large enough that the employee should be aware that they...

  11. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...Resistance to antibiotics is a widely used tool in molecular biology, yet scientists rarely stop to think about...Plasmid Pages Resources on Addgene.org Browse Our Molecular Biology Reference Pages View our Plasmid Protocols...

  12. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

    Type
    Blog Post
    Published
    Sept. 21, 2023, 1:15 p.m.
    ...challenge in the best of cases. But it can be particularly challenging when your only antibody option is...al., 2013). One issue with this strategy is that large complexes may not permeate through your tissue samples...

  13. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type
    Blog Post
    Published
    Aug. 18, 2023, 4:15 p.m.
    ...This is, for the intrepid researcher looking for molecular biology tools, a very useful trait. Virus structure... Viral vectors Viruses are quite appealing as molecular biology tools… if one isn’t too bothered by the...

  15. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...referred to as TX-TL systems) have been gaining popularity as a synthetic biology tool. These systems allow... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...

  18. Plasmids 101: Origin of Replication

    Type
    Blog Post
    Published
    Nov. 11, 2020, 2:23 p.m.
    ...maintain fewer copies of plasmids if they contain large inserts or genes that create a toxic product. ...die off. Have any questions? Need something clarified? If so please, post in the comments.  References...

  20. Intro to Our Summer SciComm Series!

    Type
    Blog Post
    Published
    June 9, 2022, 1:15 p.m.
    ... all be defined as laypeople when it comes to molecular biology.  Some lay people have a high amount of...more likely to closely read an email sent out to a large listserv or one sent to you and a few other people...
Showing: 621 - 640 of 816 results