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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

We narrowed to 789 results for: mpl

Showing: 621 - 640 of 789 results

  1. Antibodies 101: Isotypes

    Type
    Blog Post
    Published
    Oct. 19, 2021, 1:15 p.m.
    ...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade... next to a different constant domain gene. For example if a B cell switches to producing IgE antibodies...

  2. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    Published
    Jan. 25, 2024, 2:15 p.m.
    ... the cis and trans activity of each entry. For example, SpyCas9a (one of the most frequently used Cas ... an advanced search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID...

  4. Form Your Own Peer Mentoring Group: A How-To Guide for Scientists

    Type
    Blog Post
    Published
    Feb. 18, 2014, 4:22 p.m.
    ...that share a similar direction or problem. For example, a group of people who are all interested in making... such as pre-reads, videos or exercises to be completed in preparation for the meeting or to be read or...

  5. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    Published
    Feb. 4, 2014, 3:50 p.m.
    ...you work with Give more informed feedback (for example, they may be able to hear you present or see your...If they say no, don’t worry about it. It is a compliment to ask someone to share their wisdom and they...

  9. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...reluctance to share their work too openly too soon or simply a lack of awareness of the benefits of preprints...can! At the time of writing, Mike Lacy was also employed by the American Society for Cell Biology in a ...

  10. What's New in CRISPR - November 2021

    Type
    Blog Post
    Published
    Nov. 18, 2021, 2:15 p.m.
    ...Xue’s lab also uses paired pegRNAs targeting complementary DNA strands to program large genomic deletions...other prime editing tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable...

  11. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...modified GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background...additional hairpin/fluorescent protein combination, for example, with a far-red FP, would increase the available...

  13. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ... not all grant duties to trusted students. For example, if the proposal call aligns with your work, your...Below are the typical sections in a NIH RFA, for example, and tips to consider while writing each section...

  14. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...several different viral vectors to choose from, for example gamma-retrovirus, lentivirus, Adenovirus, and Adeno-associated...modular and flexible system can be used to construct complex lentiviruses for modification of mammalian cells...

  16. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

    Type
    Blog Post
    Published
    July 12, 2021, 1:15 p.m.
    ...a method that uses loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a ...a colorimetric readout that can be completed in just 45 minutes. The team deposited the reverse transcriptase...

  18. Progress Towards a PAM-Free CRISPR

    Type
    Blog Post
    Published
    March 7, 2024, 2:39 p.m.
    ...enzymes   The solution to the PAM problem seems simple, in theory: find a Cas enzyme or evolve one that...engineered Cas9 with NG PAM compatibility, as the name implies (Nishimasu et. al, 2018). xCas9 was generated by...

  19. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...more about authors by exploring author pages (for example, Melina Fan’s author page). These pages show all... Key features Setting up a personalized feed is simple. You can enter as many search terms as you like...

  20. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...CRISPR systems based on Cas9 were thought to be the simplest CRISPR systems and the easiest to adapt to genome...mechanism. To prevent new editing post-HDR, repair templates should remove the PAM sequence. Many research ...
Showing: 621 - 640 of 789 results