We narrowed to 799 results for: mpl

...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade... next to a different constant domain gene. For example if a B cell switches to producing IgE antibodies...

...plasmid can give them different phenotypes. For example, this plasmid encodes a green fluorescent protein...eukaryotic cells via a virus or viral vector. One example are bacteriophages that attach to bacterial membranes...

...conditions in order to maximize the mutation rate. For example the Phage Assisted Continuous Evolution (PACE) ...interest. A gRNA is used to direct the PolI3M-nCas9 complex to a DNA site of interest, which nCas9 nicks and...

...an interview setting, this means persuading an employer that your past work has prepared you to contribute...audience and ask for their feedback before you complete your work. If you’re giving a public talk, ask...

...interact with each other. Chromatin regulation is complex and influences--directly or indirectly--many other...phosphoinositol-dependent binding of the SWI/SNF-like BAF complex to chromatin after T lymphocyte receptor signaling...

... the cis and trans activity of each entry. For example, SpyCas9a (one of the most frequently used Cas ... an advanced search option. For the former, you simply type in identifier keywords (Cas name, RefSeq ID...

...that use reverse transcriptase to synthesize complementary DNA copies of their genome upon infecting a ... These viruses have a cargo capacity of ~ 8 kb, ample room for most experimental needs, and can transduce...

...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction...targeting an exon in your gene of interest, for example. Some customization is required, but many viral...

...jRGECO1a and jRCaMP1a are widely used as well. To complement these tools, we recently developed a new orange...cell types at once using existing indicators. For example, you might want to use OCaMP to monitor Ca2+ signals...

... in March to exhibit projects at any stage of completion, from pitch to prototype. Last year’s BioBuilderClub... for a local school’s Science Department, or to simply tour the lab as a starting point into further STEM...

...system work? Mendenhall: The system is really quite simple but it offers improvements in efficiency and throughput...right thing to do to put these out there. It’s a simple solution. It would be silly not to share it as ...

...that share a similar direction or problem. For example, a group of people who are all interested in making... such as pre-reads, videos or exercises to be completed in preparation for the meeting or to be read or...

...you work with Give more informed feedback (for example, they may be able to hear you present or see your...If they say no, don’t worry about it. It is a compliment to ask someone to share their wisdom and they...

...modified GFP-dCas9 and the gRNA scaffold to enhance complex assembly, thereby decreasing the amount of background...additional hairpin/fluorescent protein combination, for example, with a far-red FP, would increase the available...

...reluctance to share their work too openly too soon or simply a lack of awareness of the benefits of preprints...can! At the time of writing, Mike Lacy was also employed by the American Society for Cell Biology in a ...

...Xue’s lab also uses paired pegRNAs targeting complementary DNA strands to program large genomic deletions...other prime editing tools, the desired edits are templated by the 3′ extension of the pegRNAs. PEDAR is capable...

...accessible, anyone could present the material. For example, Addgene’s contributions by Tyler Ford included... and matched to serve a specific audience. For example, I joined this effort later in the year and in ...

...greatly impacting the level of gene expression. For example, the synthesis of deoxychromoviridans requires ...products is needed, such as within a multi-protein complex, stabilized promoters can eliminate variability...

...250 nm), and studying endocytosis is further complicated by the reliance on pH-based biosensors used to...rapid and reversible recruitment of chromatin complexes to any genomic locus in any cell type. By varying...

...below) Because off-target sequences have less complementarity to the gRNA, they have a higher propensity ... Hiroshi, et al. "Crystal structure of Cas9 in complex with guide RNA and target DNA." Cell 156.5 (2014...