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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 813 results for: tes

Showing: 621 - 640 of 813 results

  1. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...oxygenase for chromophore formation (abundant in eukaryotes) A Near-Infrared BiFC Reporter for In Vivo Imaging...oxygenase for chromophore formation (abundant in eukaryotes) Bright monomeric near-infrared fluorescent proteins...

  2. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...prohibitively expensive for many labs. We’ve created and tested plasmids that use CRISPR/Cas9 to endogenously tag... few single base pair mutations in crRNA target sites found within the homology areas of the plasmid to...

  3. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...number of messages in our thread or remove a good 5 minutes from our phone call. If your concern is based...get. There are questions that are answerable in minutes and questions that could take weeks to resolve ...

  4. Adapting Toehold Switches to Detect Zika Virus

    Type
    Blog Post
    Published
    Aug. 30, 2016, 2:30 p.m.
    ...virus in Brazil that was correlated with greater rates of infection and rare, but severe, symptoms, including...unlike most research, but the urgent need for the test did catalyze our resolve to get the work done as...

  5. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...0.22 µm filter) or by a centrifugation step (5 minutes at 300-500 g). After removing packaging cell debris...fibronectin-enhanced retroviral transduction of primary human T lymphocytes for adoptive immunotherapy." Acute Leukemias ...

  6. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ...biology of the worm into a computer model that recapitulates how molecular biology impacts worm locomotion...generally funded on a membership model - everyone contributes each month - with only occasional supplementation...

  7. 7 Simple Strategies to Resolve Conflicts with Difficult Supervisors

    Type
    Blog Post
    Published
    Jan. 21, 2015, 1:30 p.m.
    ...might get annoyed if you schedule meetings or send updates frequently. The easiest way to meet your supervisor...Out of Supervisor Meetings by Joanne Kamens on  Bitesize Bio Good communication with your PhD supervisor...

  8. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...color change until a few months later. RevBio accelerates with help from SynBio Axlr8r We limped along ...in 2004, she accepted a commission in the United States Air Force. She left active duty to pursue a degree...

  9. Hot Plasmids: Winter 2025

    Type
    Blog Post
    Published
    March 11, 2025, 1:15 p.m.
    ...also enabled imaging of their propagation along dendrites for the first time (Figure 3). The team also used...a patient with non-neurodegenerative diseases (Diabetes & cancer). Image adapted from Wu and Wang 2024...

  10. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ... compared to other systems because the system modulates protein post-translationally, unlike many other...through interacting ligands. After an E3 ligase facilitates ubiquitination of the Halo-tagged protein in ...

  11. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...applied to PhD programs in Biology around the United States in 2021, I know that this process only became even...program you’ll be using for the interview. Having notes close to your computer with things you want to highlight...

  12. Transferable Skills: Negotiation

    Type
    Blog Post
    Published
    March 3, 2022, 2 p.m.
    ...(Can I attend this conference?) and with my lab mates on which lab jobs needed to be done (Who is responsible...your needs actually are: Maybe you only need 15 minutes of the two hour time slot, so she can take the ...

  13. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...inside and outside your field. Your colleagues, lab mates, and PI can provide feedback on your technical presentation...pressed for time, limit yourself to five to ten minutes per poster. Ask the presenter to give the “elevator...

  15. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...eliminate every cancerous cell. Even so, to pose the greatest threat, cancerous cells still need to successfully...the research community has access to all of the latest resources? In response to this challenge, Addgene...

  17. Genome-wide Screening Using CRISPR

    Type
    Blog Post
    Published
    Aug. 18, 2015, 2:30 p.m.
    ...sequence. Co-delivery of wild-type Cas9 and a gRNA generates a double-strand break in the target DNA, which...experiment, the use of appropriate controls, multiple replicates and several cell types can strengthen your results...

  18. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...using a pinhole.  However, the excitation light excites all of the fluorophores as it passes through the...the laser light sheet, typically 2-6  microns, illuminates only one thin plane of the sample surrounding...

  19. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ...pathogens to their hosts. I spoke with her about what motivates her research, the MoClo Kit she and Sylvestre ...of terminators than are commonly used as we have tested them to show that they work well in plants and ...

  20. Tips for Using BLAST to Verify Plasmids

    Type
    Blog Post
    Published
    May 29, 2014, 1:29 p.m.
    ...Aligning two nucleotide sequences is probably the fastest BLAST search to perform and will save you time ...comparing different isoforms. In addition to our favorites, there are a number of sequence alignment tools...
Showing: 621 - 640 of 813 results