We narrowed to 694 results for: ust
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TypeBlog PostPublishedApril 6, 2023, 3 p.m....targets When staining multiple targets indirectly, you must strategically plan primary/secondary antibody pairing...
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Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....and/or stopping the HRP-TMB reaction more quickly. Just make sure to incorporate the dilution factor in ... -
Hot Plasmids and Viral Preps - March 2021
TypeBlog PostPublishedMarch 23, 2021, 1:15 p.m....repository that will help epigenetic researchers custom design their own engineering plasmid tools in about... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....photostable yellow fluorescent protein to date By Justin Ng High-throughput screening methods, such as fluorescence-activated... -
CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems
TypeBlog PostPublishedJuly 23, 2020, 1:20 p.m.... is a great way to make those genome edits. But, just as important is a way to flip the switch and turn... -
A New Generation of Adenine Base Editors Improves Editing in Primary Human Cells
TypeBlog PostPublishedMay 7, 2020, 1:15 p.m....to target. In particular, their ability for more robust editing in human cell lines and primary cells makes... -
Early Career Researcher Toolbox: Finding Relevant Papers
TypeBlog PostPublishedJan. 9, 2020, 1:45 p.m....journals, and/or papers of interest. You can also just follow feeds curated by Meta. Feeds automatically... -
Split-BioID: An Improved Method for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 1, 2018, 1:57 p.m....that indirectly interact or from proteins that are just in the proximity of the bait proteins. Instead of... -
Adeno Associated Virus (AAV) for Cell and Gene Therapy
TypeBlog PostPublishedNov. 7, 2017, 1:59 p.m....understanding of disease pathogenesis will enable robust therapeutic outcomes. In contrast to other gene... -
Quickest Way to Deposit Plasmids: The Deposit Spreadsheet
TypeBlog PostPublishedMarch 8, 2017, 3:15 p.m....yeast), S. pombe (fission yeast), A. thaliana (mustard weed), O. cuniculus (rabbit), synthetic, or other... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m.... The concentration of infectious viral particles must be high enough to maximize possible outcomes. Typically... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m....Kabadi AM, Polstein LR, Thakore PI, Glass KA, Ousterout DG, Leong KW, Guilak F, Crawford GE, Reddy TE,... -
Corralling and Cornering the Goldilocks Antibody
TypeBlog PostPublishedAug. 9, 2022, 1:15 p.m....To avoid being caught in the antibody crisis, one must corral and corner the goldilocks antibodies. A goldilocks... -
Split Fluorescent Proteins for Studying Protein-Protein Interactions
TypeBlog PostPublishedMay 3, 2022, 1:15 p.m....Split-superpositive GFP reassembly is a fast, efficient, and robust method for detecting protein-protein interactions... -
Illuminating Epigenetics with A FRET Based Biosensor
TypeBlog PostPublishedNov. 19, 2015, 3:30 p.m....like the potential devastation of the palm oil industry through epigenetic effects on the Cover of Nature... -
Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m....become increasingly restricted to academic and industrial labs, but recently there has been a counter movement... -
Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)
TypeBlog PostPublishedJune 5, 2014, 3:32 p.m....challenging to compete in today’s tough academic or industry job market for biology PhD’s. I was facing the... -
Viral Vectors 101: Producing Your rAAV
TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m....by Didem Goz Ayturk and updated by Ina Ersing in August 2023 References and resources References Aurnhammer... -
PhD Applications After COVID
TypeBlog PostPublishedJan. 5, 2023, 2:15 p.m....see if the institution is right for you outside of just the program requirements: by talking to current ... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....PCR method Uses of PCR method End point PCR or just PCR Amplify a region of interest Molecular...