We narrowed to 904 results for: CAN

...essential to sharing plasmids around the globe, it can be a bit of a mystery for those who aren’t familiar...like MTAs.  Addgene does a lot to ensure that we can facilitate a fast and agile MTA process. This is ... the depositing organization signs one form that can be used for every MTA request going forward.  After...Once they've signed and returned the MTA, Addgene can move forward with shipping the material to the requestor...requestor.  Why the MTA?  The MTA process can be a bit confusing, and it’s certainly frustrating to find...Golden Stopwatch award and seek feedback as to how we can make our process easier for others.  If you’ve still...still got questions about our MTA process, you can check out our Tech Transfer Webpage, help center articles...

...strict database design (schema).  Registered users can submit new fluorescent proteins, add data to existing..., or edit inaccuracies as they find them (anyone can register with a valid email address).  One may also... is finding information on a particular FP; this can be done directly using the search bar on the homepage...addition, the FPbase Fluorescence Spectra Viewer can be used to compare excitation and emission spectra...that specific protein (for instance: EGFP).  Users can “favorite” certain proteins, or create custom protein...comparison or ease of retrieval.  These collection pages can be kept private, or shared publicly (so feel free...fpvis.org with the proteins of your choosing!), and they can be downloaded in JSON or CSV format to quickly retrieve...

... library containing lentiviral vectors the iBARs can be detected by next generation sequencing.  In order...different lengths, the density of cells that are labeled can be altered to dense (45% of cells labeled), intermediate...sparse (4%) levels. SPARC is a versatile system that can be used in many cell types and organisms including...allow scientists to measure calcineurin dynamics and can serve as a drug-screening platform to identify new...fusions with tTALE to “stain” DNA. TALE proteins can bind specific DNA sequences which brings the attached...comparison to intercalating fluorescent dyes which can cause photo-induced DNA breakage and structural deformation...deformation or photo-induced damage. These fusions can also function in high salt conditions - up to 100...

...replicate.   How can I prevent recombination in my samples? Plasmid recombination can affect viral vectors...support team at Addgene, I get to see everything that can go wrong with a plasmid. One of the most frequent... sequences. Repeating sequences within a plasmid can undergo intramolecular DNA recombination. This naturally...recombination-prone plasmids and testing your prep can save you the headache of troubleshooting failed experiments...100 μg/mL Amp), or using more nutrient rich media can improve growth and DNA yield. Test multiple, small... plasmid and the recombined vector backbone, you can try re-streaking for single colonies and testing ...MK, Rudolph C (2019) Segmented poly(A) tails significantly reduce recombination of plasmid DNA without ...

...hybridoma cell lines can produce more than one mAb (Bradbury et al., 2018), and hybridomas can lose gene expression... be neuroscience reagents and data, the platform can be expanded for any recombinant antibody tool and...compared to polyclonal or monoclonal antibodies. You can learn more about recombinant antibodies in this blog...to create such a resource, we need your help. You can participate by depositing your plasmids encoding ...sufficient number of distributions to cover costs, which can limit expansion into new services. “We appreciate...create a fully transparent resource where researchers can easily share, edit, and access the affinity reagents...

...and medium can all affect copy number. Some ori's are temperature sensitive; others ori's can be "tricked...result, stretches of DNA that are rich in A-T pairs can be separated more readily at lower temperatures and...balance between positive and negative regulation and can be manipulated with mutations in the replicon. For... Senior Scientist Marcy Patrick says researchers can ask themselves a few questions to get started: Will...mind that plasmids with low to medium copy numbers can still express massive amounts of protein given the...product. The E. coli strain Most E. coli strains can be used to propagate plasmids, but endA- E. coli ...

...to ensure high-quality library distribution. You can also use these tips as a starting point when you ...composition of the final recovered DNA. This bias can be counteracted by growing the cells on solid plates...for the Brunello library is shown in Figure 2. You can use a small PCR product because the plasmids in the...the same output: a spreadsheet of read counts. You can use these counts to confirm the representation of...plasmids that were amplified preferentially). You can also rank the plasmids by abundance, scaled to 1,... calculating the area under the curve (AUC), one can gain a sense of the uniformity of the library distribution...verifying pooled plasmid libraries. Pooled libraries can be a powerful research tool, but their proper use...

...position that requires travel can show that you are self organised, reliable, and can work independently. Practical...might not lead to a career you actually want, you can feel lost. Use this post to explore the wide range... employers. These skills show employers that you can fully investigate and solve a problem or complete...valuable skills acquired from a science degree that can be applied to many roles. These types of skills show...what is portrayed in the media and in films, which can often be misleading. Key aspects to determine are...and volunteer experiences, as well as work history can really show that you have some of the abilities listed...sectors such as forensics currently have too many applicants compared to the number of available roles). Make...

...fluorescent proteins that can be distinguished from one another and that can be imaged with the optics...there are many fluorescent protein combinations that can be imaged together, but this also means that the ...determination of whether two fluorescent proteins can be separated from each other requires knowledge of... nm / em 474 nm) and YFP (ex 514 nm / em 527 nm) can be imaged together but CFP and GFP (ex 488 nm / em...there are techniques, like spectral unmixing, which can be used to separate the fluorescent proteins, but...particularly not the most recently published ones), they can be a good starting point. In many cases it is sufficient...

...detection can be done by the naked eye. This makes cloning quite a bit easier since you can see if your...non-specific binding to proteins can inhibit their activity. ShadowR can be used as a FRET acceptor in combination... 1). Figure 1: The sea anemone's red color can be used for mimicry. Image: Bernard Spragg. Advantages...chromoprotein, after transforming them into your cells, you can simply look for blue colonies to determine which ...other substrates for screening. Thus chromoproteins can be used as markers in cases where cost and resources...chromoproteins used in the lab come from eukaryotes, they can be difficult to directly use in bacteria, where most...’ve learned what chromoproteins are and how they can be used, why not add some color into your research...