We narrowed to 614 results for: CaS;

...Alongside there is a second-line team that takes on cases requiring in-depth analysis, data review, in-house...knowledge and judgement. For particularly tricky cases, team discussions can often lead to otherwise unobtainable...patient, attentive, empathetic, and tactful. Occasionally it can also be necessary to take the lead and...

...worry about vector replication at all. In these cases, transient transfection may be sufficient for your...transfection experiments. Reporters Finally, in some cases a researcher may choose not impose selection on ...organism your experiment takes place in. In many cases, you won’t need to worry about the expression of...

...applications, such as protein isolation from serum. In this case, Protein A and Protein G will bind both the primary...indiscriminately, causing competition for binding sites. In cases like these, antibodies can be directly conjugated...simply isn’t available for a specific target. In cases such as this, an epitope such as c-Myc, GFP or V5...

...zinc-finger nucleases, TALENs, and more recently CRISPR/Cas9. Another popular targeted gene deactivation method..., relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality sequenced zebrafish ..., and their resistance (so far) to facile CRISPR/Cas9-mediated insertion of foreign DNA. Despite these...

...their data months or years before others. In some cases, typically consortia involving genomic screens, ...the originating researchers, even if they are occasionally beaten to press, as there is so much analysis...track the motion of multiple animals on different occasions via video, generating huge amounts of data. When...

...complement each other to restore the full-expression cassette. AAV’s natural ability to reassemble fragmented...sequential homology directed repairs (HDR) with CRISPR/Cas9. Donor A contains: 400bp homology arms to the site...2017) CRISPR-Mediated Integration of Large Gene Cassettes Using AAV Donor Vectors. Cell Reports 20:750–756...

... an intact sample, a cell in the case of ICC or a tissue in the case of IHC. IF is used specifically when...interest within an intact cell, tissue, or, in some cases, a whole organism. But, as with any antibody-based...

...in lentiviral or retroviral backbones. On some occasions, shRNAs are transiently expressed using either...promoters with no tissue specificity, so in some cases they can lead to toxicity and off target effects... an Old Favorite CRISPR 101: Targeting RNA with Cas13a (C2c2) Plasmids 101: The Promoter Region – Let'...

...(Mouse IgG2a) Goat anti-mouse IgG2a CASPR anti-CASPR mAb K65/35 (Mouse IgG1) Goat anti-mouse...Neocortex labeled with anti-pan-Nav (magenta), anti-CASPR (green), and anti-Kv2.1 (cyan). (B) Cerebellum labeled...

...plasmid with repeating sequences. In some specific cases, multimerization has been observed to occur more...reduce the frequency of multimerization, and in some cases completely prevent the formation of plasmid multimers...Sharratt, 1984; Summers et al., 1993). In these cases, screening multiple bacterial colonies using one...