We narrowed to 803 results for: Cre

...CRISPR, and the challenges of science becoming increasingly politicized. It would be nearly impossible for...all of these things contributed to the steadily increasing distrust the general public has in science and...self-promotion, and personality is playing an increasingly important role in science. The science produced...reserved Franklins and Prashers will find it increasingly difficult to compete (for funding, etc.) with...chapter on deep learning addresses the dilemma of creating a technology that could eventually replace scientists...

...newest line of defense against bacteria. Created with BioRender.com.   The problem of antibiotic...may also induce cell death. CRISPR also enables increased specificity - rather than targeting a process ...mouse skin colonization model, phagemid treatment decreased the proportion of virulent S. aureus from 50% ...death. Image from Beisel et al., licensed under a Creative Commons Attribution 4.0 International License....rendering the population sensitive to antibiotics. Created with BioRender.com. Phages could be used to...

... shalt create community to help Involve family. If you don’t have local family to help, create “fake” ... on the Society for Laboratory Automation and Screening (SLAS) Electronic Laboratory Neighborhood.   Work... to church, join a club, meet the neighbors and create a network of support to help with elder care, carpooling...partner – no kids. Get a massage – no guilt. Have recreational time with your friends – no spouses or kids....

...is possible with efficiency >70%. CRMAGE is an incredibly fast protocol, with only 5 hours incubation time...system that combines recombineering with CRISPR to create a system for scarless, iterative genome engineering... pathways within this genus, Huimin Zhao’s lab created two “pCRISPomyces” systems for use in Streptomyces...CRISPR in bacteria, they also led early efforts creating tools for CRISPRi and CRISPRa (Bikard et al., ... in Mycobacterial species, Sarah Fortune’s lab screened 11 other Cas9 orthologs to identify a more efficient... used to engineer the bee gut microbiome. They created a modular system using a broad host range plasmid...

...to solve this problem. This tool was initially created to support the CRISPR analysis needs of Synthego... sgRNAs. Since multiplexing is commonly used to create functional knockouts or large deletions, the ability...optimizing and no complicated steps to learn. For increased flexibility and scalability, the ICE software ...editing analysis Once the analysis is complete, a new screen appears with a graphical representation of the ...Data button on the bottom right of the analysis screen. ICE analysis details Contributions   The Contributions...

...careers. External collaborations are becoming increasingly important as more complex approaches to scientific...seminar to connect the imaging community and to create a platform for discussions and potential collaborations...postdoctoral work there are loads of opportunities to create communities, platforms, and to be a part of projects...your ideas and don’t be afraid to make something concrete happen. You may have more willing collaborators...make sure to share them with a short note - this creates a thoughtful way of interacting and is much more...

... ligase The Roux Lab in 2016 optimized BioID to create BioID2, a smaller and improved promiscuous biotin...was its low sensitivity. Sensitivity could be increased with higher expression levels but for many fusion...until they identified a mutant with significantly increased sensitivity that did not affect the function or...miniTurbo were developed in the Ting Lab in 2018 to increase the speed of proximity labeling by promiscuous... BASU exhibits 1,000 fold faster kinetics and increases the signal to noise ratio 30-fold compared to ...

...Dicer chops off the loop of hairpin structures to create a mature siRNA duplex. Fun fact! Endogenous miRNA...production starts with transcription of the miRNA to create pri-miRNA, which is then processed by a complex...shRNA and miRNA processing and silencing pathways. Created on BioRender.com.   Expressing shRNAs using...Highlighted blue regions show flanking genomic sequence. Created on BioRender.com. When it comes to the backbone...expression of specific mRNAs. shRNAs are a quick way to create stable knockdown cells lines, and you can usually...

... used for a variety of research purposes, like creating stable cell lines, delivering CRISPR/Cas9 to cells...vector with an integrase-deficient viral vector. Created with BioRender.com. What are integrase-deficient... episomal forms of non-integrating transgenes. Created with BioRender.com; adapted from Banasik and McCray...regions of the genome (Schröder et al., 2002), thus increasing the risk for insertional mutagenesis. This integration...remember: You can never trust a lentivirus with secrets... Because they tend to insert themselves into ...

...fluorescent protein can be truncated to increase the FRET efficiency. Creating fusions at the N- and C- termini...double the size of the protein. In addition, it increases avidity because it doubles the number of binding...non-aliphatic amino acids. However, long linkers increase the chance that a fusion protein is cleaved by...Sheridan, Douglas L., et al. "A new way to rapidly create functional, fluorescent fusion proteins: random...