We narrowed to 798 results for: KIN

...promote tumor durability instead of repressing them, making it even harder to cure cancer. To understand how...factor, vascular endothelial growth factor, and interleukin-6 (Witkiewicz et al., 2009) which contribute ...metastasis of tumors through inciting inflammation. Speaking of inflammation, a noteworthy cell type in the...Witz, Isaac P. "The tumor microenvironment: the making of a paradigm." Cancer Microenvironment 2.1 (2009...

...the design of flanking overhangs. Although there are theoretically 256 distinct flanking sequences, sequences...their recognition sequence, creating four base flanking overhangs. Since these overhangs are not part ...clone up to 7 gRNAs into one destination vector, making multiplexing easy.  Disadvantages of Golden Gate...

...by starting with the least-abundant protein and working toward the most abundant. If the abundance of your...precipitates that permanently stain the membrane, making that section of the blot unusable for reprobing...ll want to check for the primary antibody by re-blocking your membrane and re-incubating with your secondary...is the one time in western blotting that you’re looking for a “blank” result! If some signal remains, you...

...tweet in order to easily access it later. Pro tip Checking the number of retweets, likes, and comments on...recent papers that the scientific community is talking about and jumping into the discussion! Semantic...altmetric score, is also displayed in your feed. By clicking on a paper in your feed you can see suggested ...Career Research Toolbox blog posts on free tools for making scientific graphics post and social media for scientists...

...localization in cells, or even single-particle tracking of the bound complex. There are so many FPs available...TagBiFC technique allows long-term single-molecule tracking of protein-protein interactions in living cells... can even be bright enough for single-molecule tracking in live cells.  Controls  When designing experiments...Proteins Plasmids and Resources Reference Feng, S., Sekine, S., Pessino, V. et al. (2017) Improved split fluorescent...

...  Second, plasmids are easy to store and share, making recombinant antibodies the most practical choice... and producing antibodies from plasmids is much kinder to our animal friends! Finally, if we want better...improve genetically-encoded research tools, it is shocking that the plasmids and sequences for antibodies...10.1038/520295b Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B,...

...Epigenetic engineering with single-pot assembly Tracking cell-cell interactions New auxin inducible degron...Epigenetic engineering with single-pot assembly By Eric Perkins Though it’s easy enough to contemplate DNA in terms... own engineering plasmid tools in about a week. Taking advantage of Golden Gate cloning, the Haynes lab...human body and G-baToN provides versibility in tracking and modulating the interactions between cells....

... one place... well, lucky for you one is in the making by Dr. Zhuhao Wu and his colleagues! Dr. Wu and...for low-cost, high-quality antibodies for IHC. Working with collaborators and support from the Brain Research...enable scientists to have an intuitive guide for checking their staining and neuroanatomy without the fear...by submitting data, sending antibodies, and/or working with Dr. Wu please contact us here! Ava Shipman...

...another optimized platform. Gene Stacking As the name implies, gene stacking is the use of a combination of...engineering complex cellular behaviors requires making complex manipulations to cells. For example: in...reagents and strategies are not available to those working with yeast or plants. Using MoClo, or better yet...

...This disease is caused by mutations in the common cytokine receptor gamma chain (c) and is characterized ...deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable landmark study showed...AAV-based gene therapies are promising, scientists are working to overcome key challenges to their efficacy:  ...AAV vectors used to treat them. Scientists are working to overcome this issue by developing new capsids...