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We narrowed to 793 results for: KIN

Showing: 321 - 330 of 793 results

  1. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...by starting with the least-abundant protein and working toward the most abundant. If the abundance of your...precipitates that permanently stain the membrane, making that section of the blot unusable for reprobing...ll want to check for the primary antibody by re-blocking your membrane and re-incubating with your secondary...is the one time in western blotting that you’re looking for a “blank” result! If some signal remains, you...

  2. Plasmids 101: Modular Cloning

    Type
    Blog Post
    Published
    March 12, 2024, 1:15 p.m.
    ...another optimized platform. Gene Stacking As the name implies, gene stacking is the use of a combination of...engineering complex cellular behaviors requires making complex manipulations to cells. For example: in...reagents and strategies are not available to those working with yeast or plants. Using MoClo, or better yet...

  3. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ...tweet in order to easily access it later. Pro tip Checking the number of retweets, likes, and comments on...recent papers that the scientific community is talking about and jumping into the discussion! Semantic...altmetric score, is also displayed in your feed. By clicking on a paper in your feed you can see suggested ...Career Research Toolbox blog posts on free tools for making scientific graphics post and social media for scientists...

  4. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...This disease is caused by mutations in the common cytokine receptor gamma chain (c) and is characterized ...deficiency, Cystic fibrosis, Muscular Dystrophy, Parkinson’s disease, and HIV. A notable landmark study showed...AAV-based gene therapies are promising, scientists are working to overcome key challenges to their efficacy:  ...AAV vectors used to treat them. Scientists are working to overcome this issue by developing new capsids...

  5. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ... one place... well, lucky for you one is in the making by Dr. Zhuhao Wu and his colleagues! Dr. Wu and...for low-cost, high-quality antibodies for IHC. Working with collaborators and support from the Brain Research...enable scientists to have an intuitive guide for checking their staining and neuroanatomy without the fear...by submitting data, sending antibodies, and/or working with Dr. Wu please contact us here! Ava Shipman...

  6. Split Fluorescent Proteins for Studying Protein-Protein Interactions

    Type
    Blog Post
    Published
    May 3, 2022, 1:15 p.m.
    ...localization in cells, or even single-particle tracking of the bound complex. There are so many FPs available...TagBiFC technique allows long-term single-molecule tracking of protein-protein interactions in living cells... can even be bright enough for single-molecule tracking in live cells.  Controls  When designing experiments...editing (Feng Zhang Lab 2015)  Reference Feng, S., Sekine, S., Pessino, V. et al. Improved split fluorescent...

  7. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type
    Blog Post
    Published
    May 4, 2021, 1:15 p.m.
    ...  Second, plasmids are easy to store and share, making recombinant antibodies the most practical choice... and producing antibodies from plasmids is much kinder to our animal friends! Finally, if we want better...improve genetically-encoded research tools, it is shocking that the plasmids and sequences for antibodies...10.1038/520295b Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B,...

  8. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...Epigenetic engineering with single-pot assembly Tracking cell-cell interactions New auxin inducible degron...Epigenetic engineering with single-pot assembly By Eric Perkins Though it’s easy enough to contemplate DNA in terms... own engineering plasmid tools in about a week. Taking advantage of Golden Gate cloning, the Haynes lab...human body and G-baToN provides versibility in tracking and modulating the interactions between cells....

  9. Hot Plasmids: Summer 2025

    Type
    Blog Post
    Published
    Aug. 28, 2025, 1:15 p.m.
    ...approach called Simultaneous Tracking of Recombinase Activation and Clonal Kinetics (STRACK) to study stem ...While many barcoding libraries are available for tracking cell lineage, different applications require careful... can transduce neurons for retrograde labeling, making it a powerful tool for circuit tracing and other...

  10. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...Whether you’re new to in-house virus production or looking to improve your current methods, understanding ... add an additional viral passage. This involves taking your first round of virus, called P1 (or sometimes... and may need to be optimized for your purpose. Making your decision The final decision of which system...10430340260355347. Vaughn, J. L., Goodwin, R. H., Tompkins, G. J., & McCawley, P. (1977). The establishment...
Showing: 321 - 330 of 793 results