...successfully reprogram. Unfortunately scRNA-seq only captures the gene expression of cells at a single time ...analysis and the remaining cells were maintained in culture. iEP cell physiology and reprogramming efficiency... lineage and identity in direct reprogramming." Nature 564.7735 (2018): 219. PubMed PMID: 30518857. Guo...

...the public in a meaningful way, and not solely to ensure a profit. Often, these companies believe they possess...chance to carry out a comprehensive review of the literature informing the notion of “heading” in soccer as... of my thesis: a survey instrument designed to measure how individuals respond to issues of unsettled ...

...the foundation of all good communication is making sure everyone’s on the same page. So for our first post...education is education that is provided through a structured environment such as the classroom or a defined...specific audience - giving consistency, purpose, structure, and intent to the messaging - while also creating...

...colleagues and is invested in improving research infrastructure, from our educational materials to our repository...and ScFvs, based on community demand. Figure 1: Antibody against PSD-95 from the Trimmer Lab...Reproducibility: Standardize antibodies used in research. Nature 518:27–29. https://doi.org/10.1038/518027a  Resources...

...seedlings or callus (undifferentiated plant cell culture), which is great for a wide variety of applications...found in all other plants, anthocyanins.   Figure 1: An adult plant expressing RUBY. Photos by Andrew...can be selected without antibiotics. (Top left) Mature silique (Arabidopsis fruit) with fertilized developing...

...stressors, including emotional stress or changes in temperature.  “The disease was first described in 1971,” ..., it is still sometimes wrongly diagnosed as a seizure disorder.” In 2012, researchers identified the ...Thailand will receive much better health care in the future based on our studies.” Thank you to all the BeHeard...

...that we aren’t still pitching for feedback.  Your venture should continuously evolve and respond to feedback...number of experts, including Harvard/MIT faculty, venture capital and angel investors, and business development...idea has potential to make a difference, then make sure you’re able to answer every possible question related...

...that are then barcoded for identification. This mixture of barcoded fragments is known as a *library*. ...additional benefit of plexWell™’s sequential tagging procedure, the quantities of library molecules for each ...multiplexed pool. The cost of these normalization steps, measured in time and consumables, can be daunting for large...

... the library, use next-generation sequencing to ensure that the library has retained gRNA representation...produce high titer lentivirus from the library, ensure gRNA representation in the viral prep, infect target... you're interested in studying. You need to make sure your assay is scalable and robust. What you don'...

...addition to thorough ethical considerations, in making sure that the research has been adequately proven to ...unethical behavior (it’s hard to argue otherwise). To be sure, throughout the history of medical research, there...on necessary actions to review and decide on the future of genome editing technology. We must stop punting...

...formats. For example, several journals like eLife, Nature, and Cell use  Research Resource identifiers (RRIDs...resolvable compact identifier. Addgene works hard to ensure that materials available through our repository...Addgene Blog Read a blog post on Our Citations Feature Check out this post on Scientific Reproducibility...

...model organism for a few reasons: Affordable to culture and easy to work with Grows extremely fast (doubling...induced by removing methanol from the media. This feature makes the strain tractable for peroxisomal studies... for genome engineering. A unique evolutionary feature of K. phaffii, relative to S. cerevisiae, is that...

...receiving only 1 TF expression construct. Cells were cultured following a differentiation protocol before performing...so that a single TF was delivered to each cell, future applications could deliver multiple TFs to each...on the Addgene Blog Learn More about Lentivirus Figure out How to Get the Most from Your Lentiviral Transduction...

...discuss those ideas and build from those.   Figure 1: Virtual lab meeting? No problem! You can still...final screen can also be saved and exported as a picture. I believe that showing something different from...document can be shared and serve as a springboard for future discussion. Collaboration and creativity are pivotal...

...got along well, suggest a coffee chat in the near future. Even just one more meeting (in person or virtually... but I’ll never forget the strawberries. Grand gestures are memorable! Attend events for one organization...defunct (but fun) plug-in called Maps.  Figure 1: See a starfish? This is actually a map of Joanne...

...they also uniformly stepped back to give a big picture perspective: Dr. Yamanaka talked about his clinical...trial patients with advanced macular degeneration; Laurenz Studer discussed his work with Parkinson's Disease...presenters, it was gratifying to see that Addgene has ensured that plasmid acquisition is not a rate-limiting...

...predict protein structures, then you might know a thing or two about computer modeling. Make sure to focus on...? Or perhaps you have presented in some public lectures? These transferable skills could be meaningful...

...see the Introduction to Fluorescence Microscopy lecture at iBiology. Commonly used filter sets & relevant...this filter set and have reported brightness measurements. Here, EGFP outperforms the improved folding...variants, presumably due to the lower growth temperature. This also suggests, however, that there is no...

...processes directly affected by light. Long-term light exposure can also lead to cellular phototoxicity, and experimental...substrate like coelenterazine is supplied in the culture medium to allow RLuc8 to produce photons. The original...wavelength to specify which FP emission you’ll capture. Since the Nano-lantern system does not use external...

...results you've measured should change established theory. So, what can you do to ensure that your skills...our website and social channels to apply for our future events. In addition to this annual event, graduate...