We narrowed to 904 results for: CAN

... the complex enters the nucleus where dCas9-Mxi1 can repress gene expression. In the dark, the NLS is ...expression of dCas9). The lab demonstrated the system can also be used to downregulate multiple genes at once...modifiers such as TET1 with inactive Cas enzymes can alter gene expression via acetylation or demethylation...

... particular disease, cell type or drug treatment can browse through screens performed in relevant cell...otherwise be hard to identify. Figure 2: Users can cross-interrogate screens from the literature for...original publication via its PubMed identifier, which can also be used to search the entire CRISPR database... the term "breast cancer" returns 40 screens performed in 26 different breast cancer cell lines. Screenshot...The analytical method for score assignment and significance thresholds used by the authors to determine ...

...deposited in Addgene!” As you can imagine, answering plasmid requests can be a time-consuming chore for...benefits of sharing plasmids with us. One way we can gauge if people are happy depositors is if they deposit...

...diverse and exciting field and to find out how we can serve them better. Here’s a little bit of what we...into science education and outreach ourselves, we can a learn a lot from these ASM pros and hope to leverage...materials! Resources on the Addgene Blog Learn How You Can Use Addgene's Microbiology Pages Read Our Post on...page and, just a few weeks ago, we attended the American Society for Microbiology's annual meeting (ASM...

...made the accidental discovery that laser treatment can photoconvert GFP from green to RED! This simple technique...photoconvertible, new studies of spatiotemporal dynamics can be undertaken using available GFP lines. Like many...compartments. Sattarzadeh et al. have shown that GFP can be rapidly and efficiently photoconverted in plant...

...Cre-estrogen receptor ligand binding domain-fusion can prevent this toxicity, but with the caveat of partial...levels. These data show that multiple recombinases can be used together to designate specific patterns of...shows that the yeast recombinases KD, B2, B3 and FLP can be used together in Drosophila as a toolkit for specific...

... demonstrated in their PNAS paper that SpyLigase can be used to link affibodies or antibodies against ...13 amino acids, but SpyCatcher is a protein. You can fuse SpyCatcher onto things, but even better if one...larger protein assemblies? Howarth: Peptide tags can be very generic; you should be able to use the same...system.) Cancer and other applications Addgene: What does this technology mean in the context of cancer? Howarth...ultimate goal to improve the diagnosis of disease, and cancer in particular. They recently introduced the SpyTag... common tumor markers to subsequently capture cancerous cells expressing low levels of tumor antigen. ...big impact on our understanding and treatment of cancer. It is clear that one needs to capture a diverse...

... hopefully make your life a little easier. These can range from technical tips as in our episode on Barcoding...machine. Lab tip #1: Glove trick Putting on lab gloves can sometimes be annoying. To make it easier, try this...Lab tip #3: Barcode everything Use barcodes and scanners to keep track of reagents in your lab. With barcode...

...understand the power of a physical model. Until then, you can only wonder what I am talking about. How do researchers...   Seeing is believing. And in that sense, models can be both useful, and dangerous. Subscribe to CRISPR...we have when we jump into a new modeling project can be a dangerous thing. Fortunately, Jacob was able...physical model is an effective teaching tool. You can read about Jacob’s initial reaction to this model...flexibility that the Cas9 protein must have in order to scan along the double-stranded DNA, recognize a PAM sequence...

...public. You can find out more about Clare at the Biology Dept. site.  References 1. American Association... mutations accumulated over time? Undergraduates can help to answer this question! The Pathways Over ...deletion). If the phenotype is corrected, students can be relatively confident that the gene from the test...epitope tags to the C-termini of Met proteins, which can be used to confirm protein expression by western ...undergraduate students (1, 2), but educators face significant challenges in designing suitable projects. In...cerevisiae deletion strains are ∆ura3 mutants and cannot grow without uracil supplementation (6). Expression... Students who participate in the course show significant gains in both content knowledge and research ...

...healthy donor cells can be delivered immediately, saving precious time. CRISPR can aid in editing these...There can be no doubt that CRISPR/Cas9 technology has been a breakthrough for the genome-editing field...delivery directly to the patient. Patient-derived cells can alternatively be harvested, edited ex vivo, and then...Therapies Cancer and CAR-T therapy The FDA has already approved CAR-T immunotherapy for cancer treatment...therapies that are currently approved or have made significant progress in clinical trials.     Figure...graft vs. host) as well as the introduction of the cancer-fighting component, CAR. Clinical trials are underway... CRISPR’s capacity for both classes of edits in cancer therapy. Blood disorders Two blood disorders are...

...into a single FASTA sequence that our QC scientists can easily analyze (see below for what we look out for...The full details of that workflow and its analysis can be found here: A Novel Next-Generation Sequencing... library in question. We will order primers that can amplify the insert of the library. This PCR step ...sequencing as well as unique barcodes per sample so we can assign sequencing reads to the correct sample. The...Addgene, each part of this new process involves a significant amount of QC. First, we perform Picogreen quantification...

...Pulse Stimulator, which can control output of several lasers simultaneously, can be used to set the laser...to use to determine the ideal excitation). Lasers can be purchased through Laserglow, which caters to optogenetics.... However, an important caveat is that one laser can stimulate only a single animal during the course ...cement (a similar procedure as implanting a guide cannula). A fiber optic patch cable is then connected from...portion of optogenetics (though it does require a significant amount of equipment, most of which is relatively...point; the fiber optics need to be polished to significant smoothness to prevent drastic loss of efficiency...stimulation, the rate of movement of the animal significantly increases. Before you get your experiment started...

...is, we’re not only sharing them with you, but you can share them with your coworkers, labmates, and friends...more than plasmids these days. via GIPHY   You can see for yourself at giphy.com/addgene. But that’s... interest).   Blugene is so excited they can’t stop dancing! via GIPHY   Don’t forget to check...

... for these methods can be found on Addgene’s stem cell page. On this page, you can also find a table with...vitro is known as reprogramming [1]. The process can be divided into two stages: Dedifferentiation - ...cell type of choice. The four reprogramming factors can be delivered and expressed in multiple somatic cells... of somatic cells. The goal is to use cells that can be easily isolated from donors. Apart from fibroblasts...Otic hair cells [31] Neurons [32, 33, 34] You can find a variety of plasmids for differentiation here...the Stem Cell Field Learn how lentiviral vectors can be used for gene delivery Resources on Addgene.org...pluripotent stem cells via temporal modulation of canonical Wnt signaling. Proc Natl Acad Sci U S A, 2012....

...last year less than 30% of applicants received sponsorship) then he or she can start working in October ...this case.  You can still assist your PI in the peer review process, but remember you cannot use it as documentary...responsibilities during their careers - being aware of this can help take the pressure off having to find the perfect...process or quota like industry, these organizations can be a good choice for individuals needing sponsorship...organization – this is not referring to one that you can pay for, but one that you are invited to join (selected...attractive option.    Your Expert Letters of support can mean that your case gets approved, denied or a Request...who has cited your work. Immediate steps that you can take to prepare for the green card process Update...

...interesting question: why engineer a new Cas9 when you can just find one that naturally occurs? This is different...functions at temperatures much warmer than SpCas9 can tolerate. Harrington et al tested GeoCas9’s love ...cells Besides being able to take some heat, GeoCas9 can introduce genomic edits in mammalian cells. Harrington...

...research to explore a variety of phenomena, and so too can it be used to teach a variety of science concepts...deeper understanding of scientific concepts than can be gained from lectures alone. Using plants as an...Resources on the Addgene Blog Learn how BioBuilder can bring Synthetic Biology into the classroom Engineering...Arabidopsis in the classroom With funding from the American Society of Plant Biologists and support from the...

...IgnaviCas9 here! What can you do with IgnaviCas9? Why would a thermostable Cas9 be useful? It can enable applications...nuclease activity is desired and time or protocol steps can be saved by flexibly deploying IgnaviCas9 without...

... fire at a certain frequency, and this frequency can indicate behavioral state, for example, if someone...quantitation. The advantage of this method is that it can be performed in a live awake animal by surgical implantation...offers immediate measurements in a live animal that can be correlated to behavior, the technique is relatively...